In vitro leishmanicidal, antibacterial and antitumour potential of anhydrocochlioquinone A obtained from the fungus Cochliobolus sp.

Abstract

The bioassay-guided fractionation of the ethyl acetate extract of the fungus Cochliobolus sp. highlighted leishmanicidal activity and allowed for anhydrocochlioquinone A (ANDC-A) isolation. MS, 1D and 2D NMR spectra of this compound were in agreement with those published in the literature. ANDC-A exhibited leishmanicidal activity with EC value of 22.4 microgram/mL (44 mu M) and, when submitted to the microdilution assay against Gram-ositive and Gram-negative bacteria, showed a minimal inhibitory concentration against ATCC 25295 of 128 microgram/mL (248.7 mu M). It was also active against five human cancer cell lines, showing IC values from 5.4 to 20.3 mu M. ANDC-A demonstrated a differential selectivity for HL-60 (SI 5.5) and THP-1 (SI 4.3) cell lines in comparison with Vero cells and was more selective than cisplatin and doxorubicin against MCF-7 cell line in comparison with human peripheral blood mononuclear cells. ANDC-A was able to eradicate clonogenic tumour cells at concentrations of 20 and 50 mu M and induced apoptosis in all tumour cell lines at 20 mu M. These results suggest that ANDC-A might be used as a biochemical tool in the study of tumour cells biochemistry as well as an anticancer agent with durable effects on tumours.

Cytotoxicity and Nitric Oxide Production Inhibitory Activities of Compounds Isolated from the Plant Pathogenic Fungus Curvularia sp.

“…[ 27 ], B. bicolor [ 35 ], Cochliobolus sp. [ 36 ], and C. miyabeanus [ 37 ]. Stemphone ( 3 ) has been reported from other genera of the Pleosporaceae family, including Drechslera sacchari [ 38 ], B. bicolor [ 35 ], and Stemphylium sarcinaeforme [ 29 ].…”

“…Anhydrocochlioquinone A ( 4 ) has also been isolated from B. oryzae [ 30 ], B. luttrellii [ 39 ], Cochliobolus sp. [ 36 ], and Veronaea sp. (Incertae sedis) [ 40 ].…”

Cochliobolus, Drechslera, Bipolaris, Curvularia different nomenclature for one potent fungus

“…It should be mentioned that over 50% of identified bioactive compounds have been isolated from fungal endophytes [15]. Endophytic fungi are the source of many pharmaceutical compounds including those exerting antibacterial, antifungal, anticancer, antitumor, antiviral, and anti-inflammatory activities [16,17].…”

Phyto-pharmacological investigation of algae Kappaphycus alvarezii (Doty) Doty ex Silva for oral diseases (Rhodophyta)

“…Екстракти K. alvarezii викликали дуже слабке пригнічення росту клітин раку ротової порожнини (клітинні лінії KB-3-1). Про цей низький цитотоксичний ефект свідчать вищі значення IC 50 (KAEA: 106705 ± 0,41 мкг/мл, KAC: 156,074 ± 0,19 мкг/мл і KAE: 209 ± 0,21) мкг/мл) порівняно з дією цисплатину у клітинах KB-3-1 (6,90 мкг/мл) (Campos et al, 2017). Інші автори також відмічали низький цитотоксичний ефект екстрактів K. alvarezii на інші лінії клітин раку, такі як лінія раку шийки матки HeLa (Lau et al, 2014) та лінія клітин гепатоми HepG2 (Ariffin et al, 2014).…”