In Vitro Leishmanicidal Activity of Benzophenanthridine Alkaloids from Bocconia pearcei and Related Compounds

Fuchino, Hiroyuki; Kawano, Marii; Mori‐Yasumoto, Kanami; Sekita, Setsuko; Satake, Motoyoshi; Ishikawa, Tsutomu; Kiuchi, Fumiyuki; Kawahara, Nobuo

doi:10.1248/cpb.58.1047

Cited by 50 publications

(14 citation statements)

References 22 publications

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“…Similar to Macleaya spp., species in Bocconia spp. also contains alkaloids and have been used medicinally to control mange, lice, and intestinal worms, and to treat ulcers, wounds, and even Leishmaniasis [35]. Both of them have been used more and more wildly not only in human medicinal areas, but also in the stockbreeding and agriculture.…”

Section: Discussionmentioning

confidence: 99%

Integration of Transcriptome, Proteome and Metabolism Data Reveals the Alkaloids Biosynthesis in Macleaya cordata and Macleaya microcarpa

Zeng

Liu

et al. 2013

PLoS ONE

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BackgroundThe Macleaya spp., including Macleaya cordata and Macleaya microcarpa, are traditional anti-virus, inflammation eliminating, and insecticide herb medicines for their isoquinoline alkaloids. They are also known as the basis of the popular natural animal food addictive in Europe. However, few studies especially at genomics level were conducted on them. Hence, we performed the Macleaya spp. transcriptome and integrated it with iTRAQ proteome analysis in order to identify potential genes involved in alkaloids biosynthesis.Methodology and Principal FindingsWe elaborately designed the transcriptome, proteome and metabolism profiling for 10 samples of both species to explore their alkaloids biosynthesis. From the transcriptome data, we obtained 69367 and 78255 unigenes for M. cordata and M. microcarpa, in which about two thirds of them were similar to sequences in public databases. By metabolism profiling, reverse patterns for alkaloids sanguinarine, chelerythrine, protopine, and allocryptopine were observed in different organs of two species. We characterized the expressions of enzymes in alkaloid biosynthesis pathways. We also identified more than 1000 proteins from iTRAQ proteome data. Our results strongly suggest that the root maybe the organ for major alkaloids biosynthesis of Macleaya spp. Except for biosynthesis, the alkaloids storage and transport were also important for their accumulation. The ultrastructure of laticifers by SEM helps us to prove the alkaloids maybe accumulated in the mature roots.Conclusions/SignificanceTo our knowledge this is the first study to elucidate the genetic makeup of Macleaya spp. This work provides clues to the identification of the potential modulate genes involved in alkaloids biosynthesis in Macleaya spp., and sheds light on researches for non-model medicinal plants by integrating different high-throughput technologies.

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Section: Discussionmentioning

confidence: 99%

Integration of Transcriptome, Proteome and Metabolism Data Reveals the Alkaloids Biosynthesis in Macleaya cordata and Macleaya microcarpa

Zeng

Liu

et al. 2013

PLoS ONE

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“…This may be due to a difference in metabolism or bioavailability [17]. Fushino et al also showed that chelerythrine (5) was among the most promising compounds tested against L. major promastigotes [15]. Chelerythrine (5) is widely used in pharmacological studies as a PKC inhibitor, and can induce apoptosis [18,19].…”

Section: Resultsmentioning

confidence: 99%

“…One describes the activity of three natural dihydrochelerythrine analogs and one synthetic derivative on promastigotes, axenic amastigotes of Leishmania donovani, and infected macrophages [14]. The second publication reports the activity of 32 benzo[c]phenanthridines, synthetic or from natural origin, on promastigotes of Leishmania major [15]. Both studies evidenced promising activities in vitro, but no in vivo studies were described.…”

Section: Abbreviationsmentioning

confidence: 99%

In Vitro and In Vivo Activity of Benzo[c]phenanthridines against Leishmania amazonensis

Castillo

Sauvain²,

Rivaud

et al. 2014

Planta Med

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Seven benzo[c]phenanthridines, synthetic or isolated from Zanthoxylum rhoifolium root bark, were evaluated against Leishmania amazonensis axenic amastigotes. Five of them were considered leishmanicidal, with IC50 values ranging from 0.03 to 0.54 µM, and were evaluated on intramacrophagic amastigotes of L. amazonensis. Chelerythrine displayed the best activity (IC50=0.5 µM), which was in the same range as the reference compound amphotericin B (IC50=0.4 µM). In vivo studies with chelerythrine, avicine, and fagaridine on a model of mice cutaneous leishmaniasis resulted in the identification of fagaridine as the most active compound. Fagaridine decreased the parasitic burden more than 50% at the 3rd and 6th weeks after the end of treatment.

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“…They are relatively rare alkaloids in the genus Corydalis; however, they exhibit a broad range of biological and pharmacological properties, such as sanguinarine in iminium or alkanolamine form could interact with DNA and showed potential clinical utility in cancer [5]. What's more, they also showed leishmanicidal and trypanocidal [6,7], antimicrobial [8], antimicrotubule [9], anti-HBV [10], analgesic and anti-inflammatory [11,12], cytotoxic [13], mosquito larvicidal [14] and anti-proliferative [15,16] activities, but until now there is no report on the protective effects on myocardial cells.…”

Section: Introductionmentioning

confidence: 99%

Ambidalmines A–E and ambidimerine F: Bioactive dihydrobenzophenanthridine alkaloids from Corydalis ambigua var. amurensis

Yang

Liu

Jiang

et al. 2014

European Journal of Medicinal Chemistry

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In Vitro Leishmanicidal Activity of Benzophenanthridine Alkaloids from Bocconia pearcei and Related Compounds

Cited by 50 publications

References 22 publications

Integration of Transcriptome, Proteome and Metabolism Data Reveals the Alkaloids Biosynthesis in Macleaya cordata and Macleaya microcarpa

Integration of Transcriptome, Proteome and Metabolism Data Reveals the Alkaloids Biosynthesis in Macleaya cordata and Macleaya microcarpa

In Vitro and In Vivo Activity of Benzo[c]phenanthridines against Leishmania amazonensis

Ambidalmines A–E and ambidimerine F: Bioactive dihydrobenzophenanthridine alkaloids from Corydalis ambigua var. amurensis

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