In vitro-Kultur und entwicklungsphysiologische Ergebnisse an Embryonen der StabheuschreckeCarausius morosus BR

CULTURE OF INSECT EMBRYOS IN VITRO*

“…The basic components of the media vary widely. Some media (Shaw, 1956;Koch, 1964) have been formulated on the basis of chemical analysis of the egg of the particular species. Other media which have given excellent results appear to differ considerably from conditions in vivo (cf.…”

“…Takami, 1963, and general comments on culture media in Begg & Cruickshank, 1963). In those instances where pertinent physiological and biochemical data are available for the species to be cultured, it would obviously be wise to take them into consideration in development of the culture medium (see Koch, 1964). But because of the paucity of information regarding the composition of the eggs of most species, a certain amount of trial and error in the development of satisfactory media seems unavoidable.…”

“…Embryonic tissues were maintained in an active state for more than 150 days, and considerable differentiation occurred in an embryo placed in culture at an intermediate stage of embryogenesis. Late embryos (FIGURE 1 ) of the stick insect Carausius rnorosus (appendages well formed and segmented, eye pigmentation begun, closure of dorsal body wall almost completed) can complete their differentiation in a medium composed of a balanced salt solution based on chemical analysis of eggs and fortified with hemolymph and egg extracts (Koch, 1961(Koch, , 1964Seidel& Koch, 1964). Attempts to culture younger stages in the same medium met with failure (Koch, 1964).…”

“…Late embryos (FIGURE 1 ) of the stick insect Carausius rnorosus (appendages well formed and segmented, eye pigmentation begun, closure of dorsal body wall almost completed) can complete their differentiation in a medium composed of a balanced salt solution based on chemical analysis of eggs and fortified with hemolymph and egg extracts (Koch, 1961(Koch, , 1964Seidel& Koch, 1964). Attempts to culture younger stages in the same medium met with failure (Koch, 1964). Efforts to culture embryos of the cricket Gryllus (Seidel, 1938, as quoted by Koch, 1964) and the stone cricket Tachycines (Krause & Krause, 1964) also have not been successful.…”

“…Attempts to culture younger stages in the same medium met with failure (Koch, 1964). Efforts to culture embryos of the cricket Gryllus (Seidel, 1938, as quoted by Koch, 1964) and the stone cricket Tachycines (Krause & Krause, 1964) also have not been successful.…”