In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins

Sampson, Julia; Raman, Amala; Karlsen, G.; Navsaria, Harshad; Leigh, I. M.

doi:10.1078/0944-7113-00032

Cited by 104 publications

(60 citation statements)

References 23 publications

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“…I. R) Council of Scientific and Industrial Research., 1950. The extract [particularly, titrated extract of C. asiatica (TECA)] has been proven as a promising candidate for improving memory, and used for cognitive disorders and for various skin ailments (Dash et al, 1996;Sampson et al, 2001;Satyavati et al, 1976;Sharma, 1992;Sivarajan, 1994). C. asiatica is used in other folklore medicines for the treatment of leprosy, ulcer, extensive wounds, eczema etc (Duke and Ayensu, 1985;Satyavati et al, 1976;Sharma, 1992).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induction of <i>Centella asiatica</i> on human breast cancer cells

Babykutty¹,

Padikkala²,

Sathiadevan³

et al. 2010

Afr. J. Trad. Compl. Alt. Med.

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The present study evaluated the ability of methanolic extract of Centella asiatica (Linn) Urban (Umbelliferae) to induce apoptosis in different cancer cell lines. MCF-7 cells emerged as the most sensitive cell line for in vitro growth inhibitory activity. C. asiatica extract induced apoptosis in MCF-7 cells as indicated by nuclear condensation, increased annexin staining, loss of mitochondrial membrane potential and induction of DNA breaks identified by TUNEL reactivity. It is possible that the use of C. asiatica extract as a component in herbal medicines could be justifiable.

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Section: Introductionmentioning

confidence: 99%

Apoptosis induction of <i>Centella asiatica</i> on human breast cancer cells

Babykutty¹,

Padikkala²,

Sathiadevan³

et al. 2010

Afr. J. Trad. Compl. Alt. Med.

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“…The cultivation medium contained [g/L]: KCl, 0.5; KH 2 PO 4 , 15; NH 4 Cl, 4; yeast extract, 5; casamino acids, 1. Before sterilization, the medium was supplemented by the specific inducer (lrhamnose, rutin, hesperidin and naringin, 5 g/L) and 1 mL/L of trace element Vishniac solution, [20] the pH was adjusted to 6.0 and after sterilization MgSO 4 · 7 H 2 O sterile solution (10% w/v, 5 mL/L) was added.…”

Section: Methodsmentioning

confidence: 99%

Biocatalyzed Generation of Molecular Diversity: Selective Modification of the Saponin Asiaticoside

et al. 2005

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An array of different derivatives of the complex ursane-type triterpene glycoside asiaticoside (1), a saponin component isolated from the perennial herb Centella asiatica, was generated by exploiting the stereo-, regio-and site-selectivity of four groups of enzymes (glycosidases, glycosyltransferases, lipases and laccases). A library of extracellular a-l-rhamnosidases (31 different items) was obtained by screening 16 different fungal strains under different cultivation conditions, and several of these preparations catalyzed the derhamnosylation of asiaticoside. Specifically, the enzymes produced by Fusarium oxysporum were selected for the synthesis of derhamno-degluco-asiaticoside (2) and also of derhamno-asiaticoside (3), by in situ glucose-inhibition of contaminating bd-glucosidases. b-1,4-Galactosyltransferase from bovine milk was subsequently used for the galactosylation of the two asiaticoside derivatives 2 and 3, using 20% v/v DMSO as a cosolvent in order to increase substrate solubility. Finally, new acylated and oxidized derivatives of asiaticoside were also prepared by exploiting the lipase from Candida antarctica (Novozym 435) and the laccase from Trametes pubescens, respectively.

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“…Asiatic acid (AA), which is one of the major components of C.asiatica has been shown to possess antioxidant and antiinflammatory activities which prevents the infiltration of inflammatory cells in bronchoalveolar lavage fluid (BALF) of cigarette smoke exposure mice (Lee et al 2016). However, the extract of C.asiatica has shown significant cytotoxicity on various human cells including airway epithelial cells above certain concentration (Mohd Heikal et al 2014;Sampson et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Effects of Secretome from Dynamic 3D Cell Culture System onto Growth and Cytoprotection of Nasal Fibroblast

Lokanathan¹

2017

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ABSTRAK Model tiga dimensi (3D) menyerupai ciri-ciri persekitaran tisu asli, justeru morfologi dan isyarat-isyarat sel daripada kultur 3D selalunya lebih menyerupai fisiologi asal berbanding sel kultur dua dimensi (2D ABSTRACTThree dimensional (3D) models mimic the features of native tissue environment. Thus, morphology and signalling of cells from 3D culture are often more physiological than routine two dimensional (2D) cell culture. It is also known that the cell-secreted products have paracrine effect on other cells growth. In this experimental study, we optimised the nasal fibroblast culture on a 3D cell culture system and studied the effects of secretome from the 3D culture (3DCM) onto fibroblast growth and cytoprotection. Nasal fibroblast was isolated from human nasal turbinates. The suitable microcarrier was selected by culturing the fibroblasts in passage 3 on various types PolyGEM™ polystyrene microcarriers. Then, the cells were cultured on selected microcarrier using a 3D culture system and the conditioned medium (CM) was collected. 3DCM were supplemented to fibroblasts to study for attachment, proliferation, and cytoprotective effects against cytotoxicity of Centella asiatica. Bicinchonic Acid Assay (BCA) was performed to quantify protein amount in CMs. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed for preliminary profiling and comparison of 2DCM and 3DCM protein profile. Our study showed that the 3DCM did not significantly enhance cell attachment and proliferation. The secretome of both 2DCM and 3DCM found to have significant cytoprotective effect onto nasal fibroblast against cytotoxicity of C.asiatica extract. 3DCM had higher protein concentration than 2DCM. SDS-PAGE showed three exclusive proteins in 3DCM and four exclusive proteins in 2DCM. Future study should be conducted on utility of nasal fibroblast secretome on cytoprotection against harmful agents in environment and cytotoxicity of natural products.

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In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins

Cited by 104 publications

References 23 publications

Apoptosis induction of <i>Centella asiatica</i> on human breast cancer cells

Apoptosis induction of <i>Centella asiatica</i> on human breast cancer cells

Biocatalyzed Generation of Molecular Diversity: Selective Modification of the Saponin Asiaticoside

Effects of Secretome from Dynamic 3D Cell Culture System onto Growth and Cytoprotection of Nasal Fibroblast

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