In vitro-Ishikawa cell test for assessing tissue-specific chemical effects on human endometrium

Schaefer, W.R.; Fischer, Lara; Deppert, Wolfgang R.; Hanjalic-Beck, Aida; Seebacher, Laura; Weimer, Marc; Zahradnik, H. P.

doi:10.1016/j.reprotox.2010.02.002

Cited by 26 publications

(26 citation statements)

References 32 publications

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“…Because none of the concentrations tested diminished cell viability by 50%, it was not possible to detect the LC 50 , which represents the concentration that causes a half-maximal decrease in cell viability. Similarly, findings reported by Schaefer et al (2010) did not show any significant decrease in cell viability in an endometrial adenocarcinoma cell line, Ishikawa cells, after exposure to BPA up to 1 mM. Concerning the effect of BPA on the proliferative activity of endometrial cells, none of the concentrations used (from 1 pM to 1 mM) decreased the de novo synthesis of DNA, whereas a significant proliferative effect could be noticed in cells treated with 10 nM BPA after 48 h of exposure.…”

Section: Discussionsupporting

confidence: 77%

“…Altogether, these data show that BPA is able to modulate steroid receptors, and this could easily lead to altered proliferation and transformation of stromal cells in the endometrium (Schaefer et al 2010, Singh et al 2011. During the window of receptivity, steroid hormones facilitate the interaction between the blastocyst and the uterine epithelium via the endometrial stroma (Ghosh & Sengupta 1998, Caballero-Campo et al 2002, Bourdiec et al 2013.…”

Section: Effect Of Bpa On Secretion Of Biomarkers Of Endometrial Matumentioning

confidence: 84%

“…The primers for SYBR Green were designed and validated from the literature using gene features of the National Center for Biotechnology (NCBI) website, Primer3 and BEACON (Premier Biosoft, Palo Alto, CA, USA; Table 2). The PRB isoform primer was designed from a fragment in the coding region unique to PRB (Gargiulo-Monachelli et al 2014), whereas, because the PRA region is included in the PRB sequence, the primer for PRA-PRB was selected from a common fragment, described in Schaefer et al (2010). The primer for GADPH was chosen from the report by Cao et al (2005).…”

Section: Quantitative Rt-pcrmentioning

confidence: 99%

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Bisphenol A modulates receptivity and secretory function of human decidual cells: an in vitro study

Mannelli¹,

Szóstek²,

Łukasik³

et al. 2015

Reproduction

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The human endometrium is a fertility-determining tissue and a target of steroid hormones' action. Endocrine disruptors (EDs) can exert adverse effects on the physiological function of the decidua at the maternal-fetal interface. We examined the potential effects of an ED, bisphenol A (BPA), on endometrial maturation/decidualization, receptivity, and secretion of decidual factors (biomarkers). In vitro decidualized, endometrial stromal cells from six hysterectomy specimens were treated with 1 pM-1 mM of BPA, for 24 h and assessed for cell viability and proliferation. Three non-toxic concentrations of BPA (1 mM, 1 nM, and 1 pM) were selected to study its influence on secretion of cell decidualization biomarkers (IGF-binding protein and decidual prolactin (dPRL)), macrophage migration inhibitory factor (MIF) secretion, and hormone receptors' expression (estrogen receptors (ERa and ERb); progesterone receptors (PRA and PRB); and human chorionic gonadotropin (hCG)/LH receptor (LH-R)). The results showed a decrease in cell viability (P!0.001) in response to BPA at the level of 1 mM. At the non-toxic concentrations used, BPA perturbed the expression of ERa, ERb, PRA, PRB, and hCG/LH-R (P!0.05). Furthermore, 1 mM of BPA reduced the mRNA transcription of dPRL (P!0.05). Secretion of MIF was stimulated by all BPA treatments, the lowest concentration (1 pM) being the most effective (P!0.001). The multi-targeted disruption of BPA on decidual cells, at concentrations commonly detected in the human population, raises great concern about the possible consequences of exposure to BPA on the function of decidua and thus its potential deleterious effect on pregnancy.Reproduction (2015) 150 115-125

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Section: Discussionsupporting

confidence: 77%

Section: Effect Of Bpa On Secretion Of Biomarkers Of Endometrial Matumentioning

confidence: 84%

Section: Quantitative Rt-pcrmentioning

confidence: 99%

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Bisphenol A modulates receptivity and secretory function of human decidual cells: an in vitro study

Mannelli¹,

Szóstek²,

Łukasik³

et al. 2015

Reproduction

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“…A correct and timely signalling between the mother and the fetus/placenta is of paramount importance for embryo implantation and further development [7]. Hormones, such as estradiol-17 β (E2) and progesterone (P4), play key roles in the preparation of the maternal uterine decidua for the implanting blastocyst [8, 9]. Similarly, these hormones regulate the development of the embryo/fetus and the correct process of placentation [10–12].…”

Section: Introductionmentioning

confidence: 99%

Bisphenol A Altersβ-hCG and MIF Release by Human Placenta: AnIn VitroStudy to Understand the Role of Endometrial Cells

Mannelli

Ietta

Carotenuto

et al. 2014

Mediators of Inflammation

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A proper fetomaternal immune-endocrine cross-talk in pregnancy is fundamental for reproductive success. This might be unbalanced by exposure to environmental chemicals, such as bisphenol A (BPA). As fetoplacental contamination with BPA originates from the maternal compartment, this study investigated the role of the endometrium in BPA effects on the placenta. To this end, in vitro decidualized stromal cells were exposed to BPA 1 nM, and their conditioned medium (diluted 1 : 2) was used on chorionic villous explants from human placenta. Parallel cultures of placental explants were directly exposed to 0.5 nM BPA while, control cultures were exposed to the vehicle (EtOH 0.1%). After 24–48 h, culture medium from BPA-treated and control cultures was assayed for concentration of hormone human Chorionic Gonadotropin (β-hCG) and cytokine Macrophage Migration Inhibitory Factor (MIF). The results showed that direct exposure to BPA stimulated the release of both MIF and β-hCG. These effects were abolished/diminished in placental cultures exposed to endometrial cell-conditioned medium. GM-MS analysis revealed that endometrial cells retain BPA, thus reducing the availability of this chemical for the placenta. The data obtained highlight the importance of in vitro models including the maternal component in reproducing the effects of environmental chemicals on human fetus/placenta.

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“…However, it is unclear whether the downregulation of AQP5 is directly correlated with endometrial disease. In this study, we examined the role of AQP5 in cell migration in human Ishikawa cells, an endometrial adenocarcinoma cell line (12).…”

Section: Introductionmentioning

confidence: 99%

Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5

Jiang

Yan

et al. 2012

Oncology Letters

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Abstract. Aquaporin (AQP)-dependent cell migration has broad implications in angiogenesis, tumor metastasis, wound healing, glial scarring and other events requiring cell movement. There are 13 isoforms of AQP (0-12) that have been identified in mammals. It is unclear whether AQP5 plays a role in the development of endometrial cancer. We recently demonstrated that ovarian steroids may affect the expression of AQP5 in the female genital tract. In this study, we considered whether AQP5 may affect cell migration in Ishikawa cells, an adenocarcinoma cell line derived from the endometrium. The results showed that the downregulation of AQP5 results in reduced Ishikawa cell migration. The estrogen (E2) receptor in the promoter of AQP5 mediated the regulation of AQP5 expression in the normal endometrium and endometrial cancer. By contrast, the upregulation of AQP5 by E2 increased cell migration, invasion and adhesion through increased annexin-2, which is responsible for F-actin remodeling and rearrangement. E2 regulates Ishikawa cell migration by regulating the AQP5 expression.

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In vitro-Ishikawa cell test for assessing tissue-specific chemical effects on human endometrium

Cited by 26 publications

References 32 publications

Bisphenol A modulates receptivity and secretory function of human decidual cells: an in vitro study

Bisphenol A modulates receptivity and secretory function of human decidual cells: an in vitro study

Bisphenol A Altersβ-hCG and MIF Release by Human Placenta: AnIn VitroStudy to Understand the Role of Endometrial Cells

Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5

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