In vitro investigations with selective adsorbents for IgE and IgM

Behm, E.; Kuroda, T.; Yamawaki, Naokuni; Tsuda, Nobuaki; Loth, F.; Schwachula, G.; Sabrowski, E; Falkenhagen, D; Klinkmann, H

doi:10.3109/10731198709118511

Cited by 15 publications

(6 citation statements)

References 2 publications

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“…Columns based on modified PVA beads are reported to be good adsorbents for IgG, IgM and IgE (6). It is also evident from Therefore, chitosan derived from prawn and crab shell Chitin, which is cheap and abundantly available in nature, can be considered as an indegeneous substitute for the manufacture of low cost LDL-sorption systems.…”

Section: Thementioning

confidence: 99%

“…C h i t o s a n C 3 ( 1 + 4 ) 2 -a m i n o -2 -d e o x y -~ -D-Glucan, derived from prawn and crab shell chitin by greater than 85% doacetylation, inherent viocority of 5.13 for 0.5% solution in 2% acetic acid at 30°C3 was a g i f t from Central Institute of fisheries technology (Cochin INDIA). NaOH solution at6 O o C . 125000, 88% mole hydrolysed, Polysciences Inc. ) , Heparin (5000 IU/ml, Biological E. Ltd, India),Chondroitin sulphate (from leeches, Sigma USA) and all chemicals are of analytical grade.Preparation of beadsChitosan beads ( C B ) are prepared as per the procedure described elsewhere(5).…”

mentioning

confidence: 99%

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Lipoprotein Adsorption Onto Modified Chitosan Beads: Preliminary Study

Sharma

Chandy

Kumari

et al. 1993

Biomaterials, Artificial Cells and Immobilization Biotechnology

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Section: Thementioning

confidence: 99%

mentioning

confidence: 99%

Lipoprotein Adsorption Onto Modified Chitosan Beads: Preliminary Study

Sharma

Chandy

Kumari

et al. 1993

Biomaterials, Artificial Cells and Immobilization Biotechnology

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“…The tripartigen plates are suitable for quantitative immuno globulin determination [3] using the reference value supplied with each plate. Tripartigen immunodiffusion plates contain a prepared agar gel in which H-chain specific antiserum to the respective immunoglobulin is incorporated.…”

Section: Immunoglobulins Igg and Iglb1mentioning

confidence: 99%

“…A number of adsorbents use the principle of interaction between antigen and antibodies for the binding of substances to remove them from human plasma [2,3]. Most of the antibodies belong to the IgG class-others are IgM proteins.…”

mentioning

confidence: 99%

Phenyl Alanine, Tryptophan Immobilized Chitosan Beads as Adsorbents for Selective Removal of Immunoproteins

1994

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The use of adsorbents for the treatment of patients suffering from various immune diseases is still in its infancy. Therefore, the development of selective absorbents for the removal or decrease of immunoproteins from plasma is of great importance. In this study, chitosan, a natural polysaccharide having structural characteristics similar to glycosamino glycans, which is non-toxic and biocompatible, has been used for protein adsorption studies. Amino acids like phenyl alanine and tryptophan in different ratios are bonded to these polymers to observe immunoadsorption. Several layers of phenyl alanine or tryptophan have been coated covalently on chitosan beads using N2-plasma, carbodiimide or glutaraldehyde treatments. Scanning electron micrographs have revealed the surface morphological changes after such modifications. The surface modified chitosan beads have exhibited high binding affinity for gamma-globulin compared to bare beads. It is also observed that the amount of fibrinogen adsorption is reduced on modified substrate. A selective removal of IgG and IgM has also been observed with these modified matrix when tested with human plasma, using immuno diffusion methods. The modified chitosan membranes have demonstrated a reduction in platelet attachment, showing that these substrates have become more blood compatible. Hence, it appears that modified chitosan surfaces may be an excellent sorbent system for haemoperfusion due to their high binding affinity for immunoproteins and blood compatibility. Further studies are needed to determine the behaviour under clinical conditions.

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“…Behm [12] synthesized IgE specific PVA beads containing tryptophan ligands, and they were able to remove pathogenic IgE from plasma effectively, but the preparative procedure was difficult, especially on a large scale, and the treatment was expensive.…”

Section: Introductionmentioning

confidence: 99%

Selective Adsorbent for the Removal of Immunoglobulin E in Bronchial Asthma

Zhang

Wang

et al. 2008

Artificial Cells, Blood Substitutes, and Biotechnology

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Agar-gel resin with tryptophan as ligand was synthesized as an adsorbent for the removal of Immunoglobulin E (IgE) in bronchial asthma. Factors affecting the adsorption properties of the adsorbent, such as the different kind of amino acid linked to the carrier, NaOH concentration and amount of epichlorohydrin used in activation, were studied in detail. The effect of spacer on the adsorption capacity was also investigated. Results showed that the agar-tryptophan adsorbent with a spacer could enhance the adsorption capacity to 70.73% (1309.403IU/g agar-gel), which was higher than that without spacers (53%). The selectivity and hemocompatibility of the adsorbent were also examined, which showed good selectivity for IgE and satisfactory blood compatibility.

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In vitro investigations with selective adsorbents for IgE and IgM

Cited by 15 publications

References 2 publications

Lipoprotein Adsorption Onto Modified Chitosan Beads: Preliminary Study

Lipoprotein Adsorption Onto Modified Chitosan Beads: Preliminary Study

Phenyl Alanine, Tryptophan Immobilized Chitosan Beads as Adsorbents for Selective Removal of Immunoproteins

Selective Adsorbent for the Removal of Immunoglobulin E in Bronchial Asthma

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