In Vitro Investigations of Tissue-Engineered Multilayered Urothelium Established from Bladder Washings

Nagele, Udo; Maurer, Sabine; Feil, G.; Bock, Conny; Krug, Jutta; Sievert, Karl‐Dietrich; Stenzl, Arnulf

doi:10.1016/j.eururo.2008.01.072

Cited by 72 publications

(48 citation statements)

References 23 publications

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“…To accomplish such stratification, the use of a specific induction medium containing high calcium concentrations [13,14] or keratinocyte growth factor (also known as fibroblast growth factor 7) [15] is needed. However, these media are not dedicated for smooth muscle cell expansion.…”

Section: Discussionmentioning

confidence: 99%

Microstructure and cytocompatibility of collagen matrices for urological tissue engineering

et al. 2010

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RESULTS• The best cytocompatibility for both urinary tract-specific cell types was obtained with OptiMaix® (Matricel GmbH, Herzogenrath, Germany) materials.• Cell-specific phenotypes were maintained during culture as shown by immunohistochemical staining.• Furthermore, simultaneous cultivation of both cell types for 7 and 14 days significantly reduced urea permeability. CONCLUSION• These results show the potential of OptiMaix materials in tissue engineering approaches of urinary tract tissues.

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Section: Discussionmentioning

confidence: 99%

Microstructure and cytocompatibility of collagen matrices for urological tissue engineering

et al. 2010

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“…These techniques are prone to complications; therefore, the development of novel reconstruction techniques by means of tissue engineering is essential. In future clinical treatments, autologous human urothelial cells (hUCs) obtained, for instance, from bladder washing [1,2] could be used.…”

Section: Introductionmentioning

confidence: 99%

Characterizing and optimizing poly- l -lactide-co-ε-caprolactone membranes for urothelial tissue engineering

Sartoneva

Haaparanta

Lahdes-Vasama

et al. 2012

J. R. Soc. Interface.

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Different synthetic biomaterials such as polylactide (PLA), polycaprolactone and poly-Llactide-co-1-caprolactone (PLCL) have been studied for urothelial tissue engineering, with favourable results. The aim of this research was to further optimize the growth surface for human urothelial cells (hUCs) by comparing different PLCL-based membranes: smooth (s) and textured (t) PLCL and knitted PLA mesh with compression-moulded PLCL (cPLCL). The effects of topographical texturing on urothelial cell response and mechanical properties under hydrolysis were studied. The main finding was that both sPLCL and tPLCL supported hUC growth significantly better than cPLCL. Interestingly, tPLCL gave no significant advantage to hUC attachment or proliferation compared with sPLCL. However, during the 14 day assessment period, the majority of cells were viable and maintained phenotype on all the membranes studied. The material characterization exhibited potential mechanical characteristics of sPLCL and tPLCL for urothelial applications. Furthermore, the highest elongation of tPLCL supports the use of this kind of texturing. In conclusion, in light of our cell culture results and mechanical characterization, both sPLCL and tPLCL should be further studied for urothelial tissue engineering.

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“…Using UCs from bladder washings is an easy and robust method that is minimally invasive besides transurethral catheterization [1,40]. Importantly, in long-term quality controls after extensive in vitro culture up to 12 passages, we have not found any structural alterations in the chromosomes, and therefore the method is considered to be safe and reliable [41].…”

Section: Discussionmentioning

confidence: 82%

“…MSCs were isolated from bone marrow aspirates from the iliac crest of healthy volunteer adult donors (age [37][38][39][40]. The Hospital Ethics Committee approved cell harvesting of UCs and informed consent was given by the parents.…”

Section: Ethicsmentioning

confidence: 99%

Transdifferentiation of autologous bone marrow cells on a collagen-poly(ε-caprolactone) scaffold for tissue engineering in complete lack of native urothelium

Zhao

Zeiai

Ekblad

et al. 2014

J. R. Soc. Interface.

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Urological reconstructive surgery is sometimes hampered by a lack of tissue. In some cases, autologous urothelial cells (UCs) are not available for cell expansion and ordinary tissue engineering. In these cases, we wanted to explore whether autologous mesenchymal stem cells (MSCs) from bone marrow could be used to create urological transplants. MSCs from human bone marrow were cultured in vitro with medium conditioned by normal human UCs or by indirect coculturing in culture well inserts. Changes in gene expression, protein expression and cell morphology were studied after two weeks using western blot, RT-PCR and immune staining. Cells cultured in standard epithelial growth medium served as controls. Bone marrow MSCs changed their phenotype with respect to growth characteristics and cell morphology, as well as gene and protein expression, to a UC lineage in both culture methods, but not in controls. Urothelial differentiation was also accomplished in human bone marrow MSCs seeded on a three-dimensional poly(1-caprolactone) (PCL)-collagen construct. Human MSCs could easily be harvested by bone marrow aspiration and expanded and differentiated into urothelium. Differentiation could take place on a threedimensional hybrid PCL-reinforced collagen-based scaffold for creation of a tissue-engineered autologous transplant for urological reconstructive surgery.

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In Vitro Investigations of Tissue-Engineered Multilayered Urothelium Established from Bladder Washings

Cited by 72 publications

References 23 publications

Microstructure and cytocompatibility of collagen matrices for urological tissue engineering

Microstructure and cytocompatibility of collagen matrices for urological tissue engineering

Characterizing and optimizing poly- l -lactide-co-ε-caprolactone membranes for urothelial tissue engineering

Transdifferentiation of autologous bone marrow cells on a collagen-poly(ε-caprolactone) scaffold for tissue engineering in complete lack of native urothelium

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