In vitro investigation of the mechanics of fixed red blood cells based on optical trap micromanipulation and image analysis
Hongtao Rao,
Meng Wang,
Yinglian Wu
et al.
Abstract:Erythrocyte deformability correlates with various diseases. Single-cell measurements via optical tweezers (OTs) enable quantitative exploration but may encounter inaccuracies due to erythrocyte life cycle mixing. We present a three-step methodology to address these challenges. Firstly, density gradient centrifugation minimizes erythrocyte variations. Secondly, OTs measure membrane shear force across layers. Thirdly, MATLAB analyzes dynamic cell areas. Results combined with membrane shear force data reveal eryt… Show more
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