In vitro investigation of the effect of mesalazine on amyloid fibril formation of hen egg-white lysozyme and defibrillation lysozyme fibrils

Faramarzian, Masoumeh; Bahramikia, Seifollah; Shasaltaneh, Marzieh Dehghan

doi:10.1016/j.ejphar.2020.173011

Cited by 16 publications

(11 citation statements)

References 28 publications

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“…Moreover, other studies have shown prolongation of the lag phase of insulin aggregation [33,34]. Our previous study demonstrate that Mesalazine, in all concentrations, especially in 1:1 ratio and higher (drug to protein), had a strong inhibitory effect on lysozyme protein brillation It has been shown a direct role of aromatic ring and OH groups around the ring in the inhibitory activity of Mesalazine [15]. After con rming the inhibitory effect of Mesalazine on insulin bril formation, the effects of the compound on preformed amyloid brils was investigated.…”

Section: Discussionmentioning

confidence: 79%

“…Mesalazine can be very important therapeutically in the development of new molecules that prevent the accumulation of insulin and other localized amyloidosis. In previous study, we investigated the effects of Mesalazine on the formation and elimination of amyloid brils from lysozyme proteins, in vitro [15]. According to the potent antiamyloidogenic properties of this compound on lysozyme protein, we decided to investigated the in vitro inhibitory and destabilizing effects of Mesalazine on human insulin protein brillation.…”

Section: Introductionmentioning

confidence: 99%

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Mesalazine Inhibits Amyloid Formation and Destabilizes Pre-formed Amyloid Fibrils in the Human Insulin

et al. 2023

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Amyloid formation due to protein aggregation is associated with several amyloid diseases (amyloidosis).The use of small organic ligands as inhibitors of protein aggregation is an attractive strategy to treatments for these diseases. In the present study, we evaluated the in vitro inhibitory and destabilizing effects of Mesalazine on human insulin protein brillation. To induce brillation, human insulin was incubated in 50 mM glycine buffer (pH 2.0) at 50°C. The effect of Mesalazine on insulin amyloid aggregation was studied using spectroscopic, imaging, and computational approaches. Based on the results, the Mesalazine in a concentration dependent manner (different ratios (1:0.1, 1:0.5, 1:1 and 1:5) of the insulin to Mesalazine) prevented the formation of amyloid brils and destroyed pre-formed brils. In addition, our molecular docking study con rmed the binding of Mesalazine to insulin through hydrogen bonds and hydrophobic interactions. Our ndings suggest that Mesalazine may have therapeutic potential in the prevention of insulin amyloidosis and localized amyloidosis. Highlights• Mesalazine stabilize the native state of human insulin prevents insulin brillization.• Mesalazine destabilize the pre-formed amyloid brils in a concentration-dependent manner.• Mesalazine can be proposed as a candidate for the treatment of amyloid diseases

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Mesalazine Inhibits Amyloid Formation and Destabilizes Pre-formed Amyloid Fibrils in the Human Insulin

et al. 2023

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“…The mutations caused in HEWL protein are the main reason for genetic amyloidosis syndromes. 72 The amyloid fibrils that mount up inside due to the protein folding create natural soluble forms and misfold to generate insoluble β-sheet-rich structures. 73 We have investigated the formation of HEWL aggregates and the inhibition of aggregation in the presence of Q conjugates.…”

Section: Resultsmentioning

confidence: 99%

Anti-amyloidogenic property of gold nanoparticle decorated quercetin polymer nanorods in pH and temperature induced aggregation of lysozyme

et al. 2022

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“…5‐aminosalicylic acid (5‐ASA) is an isomer of (4‐ASA) with a similar molecular structure, also known as mesalazine, which is a synthetic drug of non‐steroidal anti‐inflammatory drugs, and it can be used for the treatment of gastrointestinal inflammatory diseases [8]. According to research, the pharmacological and biological effects of 5‐ASA include the treatment of inflammatory bowel disease [9], as well as antioxidant [10], anti‐inflammatory [11], antibacterial [12], anticancer [13], anti‐amyloid [14], gastroprotective and anti‐fibrotic properties [15]. It can be seen that although 4‐ASA and 5‐ASA are isomers of each other, due to the different substitution positions of the amino groups, the structural differences lead to differences in the functions of the two.…”

Section: Introductionmentioning

confidence: 99%

“…T A B L E 1 The electron energies of 4-ASA and 5-ASA under the gas phase and solvent models B: COH (À15); B: OHO(14) Note: The values in parentheses represent the contribution of vibration modes, and a negative sign represents the opposite vibration phase. Abbreviations: B, bond angle bending; S, bond length stretching; T, dihedral angle torsion.…”

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confidence: 99%

Qualitative and quantitative study of intermolecular weak interactions for aminosalicylic acid isomers by terahertz spectroscopy

Tang

et al. 2022

Int J of Quantum Chemistry

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In the field of chemical science, the development of new drugs, the formation of crystals and molecular clusters, and the optimization of drug safety and efficacy are all closely related to weak molecular interactions. In this paper, the salicylic drugs 4-aminosalicylic acid (4-ASA) and 5-aminosalicylic acid (5-ASA) are used as the research objects. First, the terahertz (THz) absorption spectra of the two isomers were measured in the range of .4-2.0 THz by terahertz time-domain spectroscopy (THz-TDS). Then the unit cells were calculated by using density functional theory (DFT). We found that the characteristic absorption peaks of the experimental spectra were basically consistent with the theoretical calculation. In addition, under the solvent model, the free energy of dissolution and the free energy of the systems were considered and calculated, and the results showed that solvation had a certain influence on the position and intensity of spectral absorption peaks. In order to further explore the origin of the characteristic absorption peaks, the vibrational properties of the unit cell model were analyzed using the potential energy distribution (PED) method. At the same time, the weak interactions of the system were qualitatively and quantitatively analyzed by interaction region indicator (IRI) and energy decomposition analysis based on forcefield (EDA-FF) methods, respectively. The results show that the absorption peaks of 4-ASA and 5-ASA are mainly derived from the vibration mode of dihedral angle torsion. The weak interactions in the 4-ASA are dominated by dispersion, while they are dominated by electrostatic and dispersion in the 5-ASA. Therefore, it is an effective way to identify isomers and study intermolecular weak interactions by using THz-TDS combined with PED, IRI, and EDA-FF.

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In vitro investigation of the effect of mesalazine on amyloid fibril formation of hen egg-white lysozyme and defibrillation lysozyme fibrils

Cited by 16 publications

References 28 publications

Mesalazine Inhibits Amyloid Formation and Destabilizes Pre-formed Amyloid Fibrils in the Human Insulin

Mesalazine Inhibits Amyloid Formation and Destabilizes Pre-formed Amyloid Fibrils in the Human Insulin

Anti-amyloidogenic property of gold nanoparticle decorated quercetin polymer nanorods in pH and temperature induced aggregation of lysozyme

Qualitative and quantitative study of intermolecular weak interactions for aminosalicylic acid isomers by terahertz spectroscopy

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