In vitro investigation of methylene blue-bearing, electrostatically assembled aptamer–silica nanocomposites as potential photodynamic therapeutics

Tian, Ding; Luo, Yun; Hsu, Hsin-Yun

doi:10.1016/j.colsurfb.2015.07.064

Cited by 13 publications

(11 citation statements)

References 40 publications

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“…Although some GQDs can enter cells without modification (32), many graphene quantum dots are not able to cross cancer cell membranes (33)(34)(35)(36)(37)(38). Similarly, methylene blue can also exhibit reduced membrane permeability in cancer cell lines (18,(39)(40)(41)(42). Our data (Fig.…”

Section: Cell Uptake Measurementmentioning

confidence: 72%

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Monroe

Belekov

et al. 2019

Photochem & Photobiology

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Photodynamic therapy (PDT) is a field with many applications including chemotherapy. Graphene quantum dots (GQDs) exhibit a variety of unique properties and can be used in PDT to generate singlet oxygen that destroys pathogenic bacteria and cancer cells. The PDT agent, methylene blue (MB), like GQDs, has been successfully exploited to destroy bacteria and cancer cells by increasing reactive oxygen species generation. Recently, combinations of GQDs and MB have been shown to destroy pathogenic bacteria via increased singlet oxygen generation. Here, we performed a spectrophotometric assay to detect and measure the uptake of GQDs, MB and several GQD‐MB combinations in MCF‐7 breast cancer cells. Then, we used a cell counting method to evaluate the cytotoxicity of GQDs, MB and a 1:1 GQD:MB preparation. Singlet oxygen generation in cells was then detected and measured using singlet oxygen sensor green. The dye, H2DCFDA, was used to measure reactive oxygen species production. We found that GQD and MB uptake into MCF‐7 cells occurred, but that MB, followed by 1:1 GQD:MB, caused superior cytotoxicity and singlet oxygen and reactive oxygen species generation. Our results suggest that methylene blue's effect against MCF‐7 cells is not potentiated by GQDs, either in light or dark conditions.

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Section: Cell Uptake Measurementmentioning

confidence: 72%

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Monroe

Belekov

et al. 2019

Photochem & Photobiology

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“…NP properties could be modified by the polyvalent effect of MUC1 aptamers, which may lead to better binding activity. Increasing the number of aptamers on the surface of silica nanocomposite and lipid-polymer hybrid NPs increases multiple binding and synergistic affinity, 47,75 while increased numbers of aptamers on the surface of ELR polyplexes decreases transfection efficiency by the polyplex at an N/P ratio of 50 and has no effect on transfection efficiency at polyplex/aptamer at N/P ratios of 5. 78 These results indicate that the number of aptamers attached to each NP should be accurately determined.…”

Section: Resultsmentioning

confidence: 99%

“…The phototoxicity of APT–MB–SiNPs was calculated using the CellTiter Blue Cell Viability Assay. The APT–MB–SiNPs significantly decreased cell viability in an MUC1+ cell line when compared to noncancerous normal kidney and MUC1‐cells …”

Section: The Muc1 Aptamer In Targeted Drug Deliverymentioning

confidence: 99%

Anti‐MUC1 aptamer: A potential opportunity for cancer treatment

Nabavinia

Gholoobi

Charbgoo

et al. 2017

Medicinal Research Reviews

112

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“…Importantly, the reduced form of MB exhibits negligible photodynamic efficiency [ 14 , 15 ]. It has been earlier demonstrated that the use of carriers for MB molecules, such as silica nanocomposites [ 16 ] or calcium phosphate nanoparticles [ 17 ], may improve its photodynamic efficacy in vitro and protect the PS from the enzymatic reduction.…”

Section: Introductionmentioning

confidence: 99%

Complexing Methylene Blue with Phosphorus Dendrimers to Increase Photodynamic Activity

et al. 2017

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The efficiency of photodynamic therapy is limited mainly due to low selectivity, unfavorable biodistribution of photosensitizers, and long-lasting skin sensitivity to light. However, drug delivery systems based on nanoparticles may overcome the limitations mentioned above. Among others, dendrimers are particularly attractive as carriers, because of their globular architecture and high loading capacity. The goal of the study was to check whether an anionic phosphorus dendrimer is suitable as a carrier of a photosensitizer—methylene blue (MB). As a biological model, basal cell carcinoma cell lines were used. We checked the influence of the MB complexation on its singlet oxygen production ability using a commercial fluorescence probe. Next, cellular uptake, phototoxicity, reactive oxygen species (ROS) generation, and cell death were investigated. The MB-anionic dendrimer complex (MB-1an) was found to generate less singlet oxygen; however, the complex showed higher cellular uptake and phototoxicity against basal cell carcinoma cell lines, which was accompanied with enhanced ROS production. Owing to the obtained results, we conclude that the photodynamic activity of MB complexed with an anionic dendrimer is higher than free MB against basal cell carcinoma cell lines.

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In vitro investigation of methylene blue-bearing, electrostatically assembled aptamer–silica nanocomposites as potential photodynamic therapeutics

Cited by 13 publications

References 40 publications

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Anticancer Photodynamic Therapy Properties of Sulfur‐Doped Graphene Quantum Dot and Methylene Blue Preparations in MCF‐7 Breast Cancer Cell Culture

Anti‐MUC1 aptamer: A potential opportunity for cancer treatment

Complexing Methylene Blue with Phosphorus Dendrimers to Increase Photodynamic Activity

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