In vitro intestinal permeability studies, pharmacokinetics and tissue distribution of 6-methylcoumarin after oral and intraperitoneal administration in Wistar rats

Cárdenas, Paola Andrea; Kratz, Jadel M.; Hernández, Aura Rocio; Costa, Geison Modesti; Ospina, Luís Fernando; Baena, Yolima; Simões, Cláudia Maria Oliveira; Jiménez-Kairuz, Álvaro F.; Aragón, Marcela

doi:10.1590/s2175-97902017000116081

Cited by 19 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prevalence of these two different kinds of diseases could be because they are common in the study area, or medicinal plants are more useful and have potent activity against digestive problems. We may assert that traditional herbal therapies may play an influential role in treating digestive tract problems, as they are the target of valuable activity [ 30 ]. Some of the most important and commonly used plants included Withania coagulans (33 URs), Piper nigrum (33 URs), and Thymus linearis (32 URs), Olea europaea subsp.…”

Section: Resultsmentioning

confidence: 99%

Keeping their own and integrating the other: medicinal plant use among Ormurs and Pathans in South Waziristan, Pakistan

Aziz,

Hassan,

Ullah

et al. 2023

J Ethnobiology Ethnomedicine

View full text Add to dashboard Cite

Background In multicultural societies, traditional knowledge among minorities faces several challenges. Minority groups often face difficult situations living in specific peripheral geographies and striving to retain their biocultural heritage, including medicinal plant knowledge and practices. Folk medicinal plant knowledge is a dynamic eco-cultural complex influenced by various environmental, socio-cultural, and political factors. Examining medicinal plant knowledge among minorities has been an increasingly popular topic in cross-cultural ethnobiology. It also helps understand the dynamics of local/traditional ecological knowledge (LEK/TEK) change within a given community. The current study was designed to investigate the status of medicinal plant knowledge among two linguistic groups, i.e. Ormurs and Pathans, living in a remote valley of West Pakistan. Methods We recruited 70 male study participants from the studied groups for semi-structured interviews to record the medicinal plant use of their communities. Data were compared among the two studied communities using the stacked charts employing the presence or absence of data with Past 4.03 and Venn diagrams. Use reports (URs) were counted for each recorded taxon. Results and Discussion A total of seventy-four medicinal plants were quoted as used as ethnomedicines by the researched communities. Most of the reported plants were used to treat digestive and liver problems. The cross-cultural comparison revealed a considerable homogeneity of medicinal plant knowledge (the two groups commonly used more than seventy plants); however, comparing uses recorded for the widely utilised medicinal plants showed numerous idiosyncratic uses among Ormurs but very few among Pathans. Ormurs reported a higher number of cultivated, wild, and imported plant uses than did Pathans. These results indicate that, compared to Pathans, the Ormur linguistic minority retain more folk medicinal plant knowledge, which may be explained by the fact that they have incorporated different folk remedies: their “own knowledge” plus that of Pathans, with whom they have lived together for centuries. Moreover, the local plant nomenclature among Ormurs was highly affected by the plant nomenclature of Pathans. Conclusion The current study revealed that living together for a few centuries has not implied sharing plant knowledge (as the Pathans do not seem to have learnt from the Ormurs) or, in other words, that plant knowledge exchanges have been unidirectional. The findings show that the Pashto dominant culture may have possibly put pressure on the minority groups and affected local plant-centred cultural practices, as we see in the case of local plant nomenclature hybridisation among Omuri speakers. Hence, it is imperative to employ diverse educational strategies to revitalise the decline of medicinal plant knowledge in the studied communities, especially among Ormurs, who need more attention as they face more challenges than the other group. Locally based strategies should be devised to restore the fading connection with nature, which will be advantageous for revitalising plant knowledge.

show abstract

Section: Resultsmentioning

confidence: 99%

Keeping their own and integrating the other: medicinal plant use among Ormurs and Pathans in South Waziristan, Pakistan

Aziz,

Hassan,

Ullah

et al. 2023

J Ethnobiology Ethnomedicine

View full text Add to dashboard Cite

show abstract

“…Experiments were held in triplicates. The apparent permeability coefficient (P app ) of PIT solution and the optimized bilosomes was then calculated using equation (4) (Cárdenas et al, 2017 ). where: P app : Apparent permeability coefficient (cm/s) dc/dt: Cumulative concentration of drug (c) transported to the basolateral chamber as a function of time (t) and was estimated from the slope of the linear portion of the concentration vs. time plot ▪ A: Surface area of the monolayer filter C 0 : Initial concentration of drug in the apical chamber (μg/mL) …”

Section: Methodsmentioning

confidence: 99%

Pitavastatin-loaded bilosomes for oral treatment of hepatocellular carcinoma: a repurposing approach

et al. 2022

View full text Add to dashboard Cite

Albeit its established efficacy as an anti-hyperlipidemic agent, pitavastatin (PIT) has been shown to have other various therapeutic effects. One of these effects is the anti-cancer activity against hepatocellular carcinoma (HCC). This effect has been evaluated in this study for the first time via its oral delivery loaded in bilosomes both in vitro in hepatocellular carcinoma (HCC) cell line; HepG2 and in vivo in an Ehrlich ascites carcinoma (EAC) model. Moreover, the impact of surface modification of bilosomes with lactoferrin (LF) as an active targeting ligand for HCC was investigated. Bilosomes were prepared by thin-film hydration and different molar phospholipid to bile salt ratios were used to optimize the bilosomal formulation. The molar phospholipid to bile salt ratio was adjusted to 4:1 at pH 7.4. LF-coated bilosomes possessed a particle size, PDI, entrapment efficiency, and zeta potential of 112.28 nm ± 6.35, 0.229 ± 0.06, 90.56% ± 3.22, and −7.86 mV ± 1.13, respectively. LF-coated bilosomes also increased permeation of PIT when tested on Caco-2 cells by 3.1-folds (compared to uncoated ones or free PIT solution). It also improved the cytotoxicity of HepG2 spheroids 44-folds more than PIT-free solution. RT-PCR analysis showed that LF-coated PIT-loaded bilosomes caused an improvement (2-fold increase) in the apoptotic potential of PIT mediated by caspase-3. In conclusion, the optimized LF-coated PIT-loaded bilosomes were cytotoxic to HCC with improved hepatocytes permeation and cellular uptake. Thus, the proposed formula could be a promising treatment for HCC.

show abstract

“…The average surface area of a rat’s intestine is approximately 1.25 cm 2 per cm [ 19 ], and the estimated average surface area of our everted gut sac model is 3.125 cm 2 . The apparent permeability coefficients (P app ) were calculated from Equation (3) [ 20 ]:

…”

Section: Methodsmentioning

confidence: 99%

Poly (Lactic-Co-Glycolic) Acid–Poly (Vinyl Pyrrolidone) Hybrid Nanoparticles to Improve the Efficiency of Oral Delivery of β-Carotene

Liu

Hsieh

2022

Pharmaceutics

View full text Add to dashboard Cite

The aim of this study was to develop a nanoparticle formulation made of poly (vinyl pyrrolidone) (PVP) and poly (lactic-co-glycolic) acid (PLGA) for the oral delivery of β-carotene (BC). The hybrid nanoparticles were prepared by the interfacial deposition method, and the physicochemical properties of this formulation were characterized in terms of its morphology, particle size, size distribution, encapsulation efficiency, dissolution, intestinal permeability, and in vivo pharmacokinetics. Our results demonstrated that BC-loaded nanoformulation and PLGA nanoparticles (PNP) significantly enhanced a release 6.1 times higher than BC suspension. The fortification of PVP into PLGA nanoparticles, named PLGA–PVP hybrid nanoparticles (PPNP), significantly reduced the particle size, as well as led to an increase 1.9 times higher in the in vitro release of BC, compared with PNP. For the ex vivo intestinal permeability assessment, PNP and PPNP–K15 significantly enhanced the intestinal permeability by 2.7 and 6.5 times at the jejunum, and 2.3 and 4.5 times at the ileum, when compared with unformulated BC. According to the pharmacokinetic study, the optimized hybrid formulation significantly increased the peak plasma concentration (Cmax) and the area under the curve (AUC0-t), and the oral relative bioavailability showed a five-fold enhancement compared with that of the BC suspension. Our results indicate that the hybrid nanoparticulate delivery system is an efficient strategy for the oral delivery of BC.

show abstract

In vitro intestinal permeability studies, pharmacokinetics and tissue distribution of 6-methylcoumarin after oral and intraperitoneal administration in Wistar rats

Cited by 19 publications

References 27 publications

Keeping their own and integrating the other: medicinal plant use among Ormurs and Pathans in South Waziristan, Pakistan

Keeping their own and integrating the other: medicinal plant use among Ormurs and Pathans in South Waziristan, Pakistan

Pitavastatin-loaded bilosomes for oral treatment of hepatocellular carcinoma: a repurposing approach

Poly (Lactic-Co-Glycolic) Acid–Poly (Vinyl Pyrrolidone) Hybrid Nanoparticles to Improve the Efficiency of Oral Delivery of β-Carotene

Contact Info

Product

Resources

About