In Vitro Interleukin-1 Production by Different Dialysis Membranes

Amato, Maria Pia; Cozzolino, Federico; Bergesio, Franco; Salvadori, Maurizio; Torcia, M; Carossino, Anna Maria; Sodi, Andrea

doi:10.1093/oxfordjournals.ndt.a091693

Cited by 17 publications

(8 citation statements)

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“…Factors other than the membrane such as the device and the mode of therapy may account for the differences in predialytic levels of cytokines. The possible mechanism underlying the increased pro duction of IL-lp and TNF-a during the interdialytic period may be dependent on an endotoxin stimulation of monocytes/macrophages when endotoxin-containing dialysate diffuses back into the blood compartment, rather than on the interaction between dialy sis membranes and monocytes [19], Using an ELISA specific for antibodies to Escheri chia coli endotoxin and E. coli J-5 antigen, Yamagami et al [20] showed that hemodial ysis with high-flux, high-permeable mem branes is associated with the highest percent age of patients positive for endotoxin anti body as compared to that observed with lowflux membranes. Laude et al [21] have shown that lipopolysaccharide (LPS) may in deed cross polyacrylonitrile membranes at least in vitro.…”

Section: Discussionmentioning

confidence: 99%

Production of Cytokines in Hemodialysis

et al. 1990

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Tumor necrosis factor (TNF-α) and interleukin-1 (IL-1 β) are cytokines primarily produced by monocytes/macrophages when stimulated by endotoxin, complement-derived anaphylatoxins and the specific antigen. In the present study, the plasma levels of TNF-α and IL-1β were evaluated before and after hemodialysis with cuprophane membrane (in 9 patients) and hemodiafiltration (in 9 patients) using three high-permeability membranes such as polymethylmethacrylate, polyacrylonitrile (AN-69) and polysulfone. In vitro spontaneous production of TNF-α and IL-1β was evaluated in the supernatants from short-term cultured monocytes obtained before and after treatment. The predialytic levels of TNF-α and IL-1β were significantly higher (p < 0.05) in the uremic population than in 21 healthy subjects taken as controls. The analysis of the uremic population regarding the mode of therapy indicated that in hemodialysis the predialytic plasma levels of TNF-α and IL-lβ did not significantly differ from those of healthy subjects. In contrast, in hemodiafiltration with polymethylmethacrylate and AN-69, but not with polysulfone, the predialytic plasma levels of both cytokines were significantly (p < 0.05) increased. No significant variation in plasma levels of both cytokines was observed after hemodialysis with cuprophane membranes. Hemodiafiltration with polymethylmethacrylate and AN-69, but not with polysulfone, brought about a consistent reduction in plasma levels of both cytokines. Detectable amounts of TNF-α and IL-1β were spontaneously produced by peripheral-blood monocytes 6 h after the end of hemodialysis but not of hemodiafiltration. These studies suggest a possible role of TNF-α and IL-1 β in the biocompatibility of different extracorporeal treatments.

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Section: Discussionmentioning

confidence: 99%

Production of Cytokines in Hemodialysis

et al. 1990

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“…This would be of particular importance when consid ering high-flux hemodialysis where endotoxin contami nation of dialysate is a significant clinical problem. Acti vation of lymphocytes and macrophages by dialysis membranes may further increase the production of cyto kines and their release into bone matrix and cartilage [44, [46][47][48]. The cytokines, TNFa and IL-1, exert a more powerful effect on bone resorption than parathormone, both in vitro and in vivo [44], In an exciting and unex pected development, Canalis et al [49] recently isolated a transforming growth factor from both bone matrix and calvarial cultures which they named bone-derived growth factor (BDGF).…”

Section: Cytokinesmentioning

confidence: 99%

Beta-2-Microglobulin Amyloidosis in Long-Term Dialysis Patients

Stone

Hakim²

1989

Am J Nephrol

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“…Conflicting data exist about the specific effects of different membrane materials on cytokine release, especially with regard to so-called biocompatible and nonbiocompatible membrane types. While some authors found that complement-activating (nonbiocompatible) membranes led to a higher production of cytokines and their receptors than biocompatible membranes [13, 14, 15, 16, 17, 18], others found no such differences [19]or even opposite results [20, 21]. In one study, during short-term and long-term use, polyamide dialyzers were shown not to induce changes in plasma cytokine levels at all [22].…”

Section: Introductionmentioning

confidence: 99%

Induction of Cytokines and Adhesion Molecules in Stable Hemodialysis Patients: Is There an Effect of Membrane Material?

Hoffmann¹,

Fischereder²,

Marx

et al. 2003

Am J Nephrol

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Background/Aims: The aim of this study was to examine the effects of a biocompatible and a nonbiocompatible hemodialysis membrane on the secretion of cytokines and their specific antagonists as well as on the expression of adhesion molecules. Methods: A crossover study using cuprophane (CU) and polysulfone (PS) dialyzers and measurements of serum tumor necrosis factor (TNF)-alpha, soluble TNF receptors, interleukin (IL)-6, IL-6 receptor, IL-2 receptor, IL-1 receptor antagonist and the adhesion molecules intercellular adhesion molecule 1, endothelial leukocyte adhesion molecule and vascular cell adhesion molecule was performed in 19 hemodialysis patients. 44 healthy volunteers served as controls. Results: All of the measured cytokines and cytokine antagonists as well as all adhesion molecules were significantly elevated in hemodialysis patients compared to controls. There was a significant increase in TNF-alpha during a dialysis session with a CU dialyzer, but only a moderate increase using a PS dialyzer. None of the other cytokines and adhesion molecules were changed during a dialysis session. We were also able to show an upregulation of adhesion molecules in dialysis patients. Conclusion: Our study clearly demonstrates that levels of inflammatory cytokines as well as their antagonists and adhesion molecules are elevated in patients on hemodialysis therapy. Baseline values before the start of a dialysis session did not show any differences with regard to the usage of CU or PS dialysis membranes. However, CU dialyzers led to a significantly greater stimulation of TNF-alpha during the dialysis session in comparison to PS membranes, suggesting a higher degree of bioincompatibility.

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In Vitro Interleukin-1 Production by Different Dialysis Membranes

Cited by 17 publications

References 0 publications

Production of Cytokines in Hemodialysis

Production of Cytokines in Hemodialysis

Beta-2-Microglobulin Amyloidosis in Long-Term Dialysis Patients

Induction of Cytokines and Adhesion Molecules in Stable Hemodialysis Patients: Is There an Effect of Membrane Material?

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