In vitro interactions of Acanthamoeba castellanii Neff and Vibrio harveyi

Reyes-Batllé, María; Martín‐Rodríguez, Alberto J.; López-Arencibia, Atteneri; Sifaoui, Inés; Rizo-Liendo, Aitor; Bethencourt-Estrella, Carlos J.; Mendez, A.; Chiboub, Olfa; Hajaji, Soumaya; Valladares, Basilio; Martínez-Carretero, Enrique; Piñero, José E.; Lorenzo‐Morales, Jacob

doi:10.1016/j.exppara.2017.09.003

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…According to several recent studies, the survival of C. jejuni can be enhanced in the presence of Acanthamoeba spp., which is an amoeba frequently found in water and soil ( Siddiqui and Khan, 2012 ). It was previously shown that bacterial interactions with Acanthamoeba are a defense mechanism of various pathogens, e.g., Legionella pneumophila , Vibrio spp., Pseudomonas aeruginosa , Listeria monocytogenes , Helicobacter pylori , Escherichia coli and Mycobacterium avium , and may even act as a vector for transmission of these pathogens to hosts ( Rowbotham, 1980 ; Cirillo et al, 1999 ; Winiecka-Krusnell et al, 2002 ; Reyes-Batlle et al, 2017 ). As for C. jejuni , cocultivation with the amoeba resulted in a delayed decline of bacterial numbers and a longer survival period at both sub-optimal temperatures and under AC ( Axelsson-Olsson et al, 2005 , 2010a , b ; Baré et al, 2010 ; Bui et al, 2012a ; Griekspoor et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

“…It was previously reported that co-incubation with Acanthamoeba resuscitates C. jejuni cultures ( Axelsson-Olsson et al, 2005 , 2007 ; Reyes-Batlle et al, 2017 ), provides C. jejuni with increased acid resistance ( Axelsson-Olsson et al, 2010a , b ), protects it from chlorine and other chemical disinfectants ( King et al, 1988 ; Snelling et al, 2005 ), and improves the survival of C. jejuni in milk and orange juice ( Olofsson et al, 2015 ). However, the molecular mechanisms of interactions between C. jejuni and amoebas are not well understood, and the results of related publications are often conflicting.…”

Section: Introductionmentioning

confidence: 99%

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Waterborne Isolates of Campylobacter jejuni Are Able to Develop Aerotolerance, Survive Exposure to Low Temperature, and Interact With Acanthamoeba polyphaga

2021

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Campylobacter jejuni is regarded as the leading cause of bacterial gastroenteritis around the world. Even though it is generally considered to be a sensitive microaerobic pathogen, it is able to survive in the environment outside of the intestinal tract of the host. This study aimed to assess the impact of selected environmental parameters on the survival of 14 C. jejuni isolates of different origins, including 12 water isolates. The isolates were tested for their antibiotic resistance, their ability to survive at low temperature (7°C), develop aerotolerance, and to interact with the potential protozoan host Acanthamoeba polyphaga. The antibiotic susceptibility was determined by standard disk diffusion according to EUCAST. Out of the 14 isolates, 8 were resistant to ciprofloxacin (CIP) and 5 to tetracycline (TET), while only one isolate was resistant to erythromycin (ERY). Five isolates were resistant to two different antibiotic classes. Tetracycline resistance was only observed in isolates isolated from wastewater and a clinical sample. Further, the isolates were tested for their survival at 7°C under both aerobic and microaerobic conditions using standard culture methods. The results showed that under microaerobic conditions, all isolates maintained their cultivability for 4 weeks without a significant decrease in the numbers of bacteria and variation between the isolates. However, significant differences were observed under aerobic conditions (AC). The incubation led to a decrease in the number of cultivable cells, with complete loss of cultivability after 2 weeks (one water isolate), 3 weeks (7 isolates), or 4 weeks of incubation (6 isolates). Further, all isolates were studied for their ability to develop aerotolerance by repetitive subcultivation under microaerobic and subsequently AC. Surprisingly, all isolates were able to adapt and grow under AC. As the last step, 5 isolates were selected to evaluate a potential protective effect provided by A. polyphaga. The cocultivation of isolates with the amoeba resulted in the survival of about 40% of cells treated with an otherwise lethal dose of gentamicin. In summary, C. jejuni is able to adapt and survive in a potentially detrimental environment for a prolonged period of time, which emphasizes the role of the environmental transmission route in the spread of campylobacteriosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Waterborne Isolates of Campylobacter jejuni Are Able to Develop Aerotolerance, Survive Exposure to Low Temperature, and Interact With Acanthamoeba polyphaga

2021

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“…Other studies have also demonstrated that the culture of Campylobacter jejuni , Vibrio harveyi , or Salmonella typhi in the presence of Acanthamoeba spp. increases bacterial survival (Douesnard‐Malo & Daigle, 2011; Reyes‐Batlle et al, 2017; Reyes‐Batlle et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the occurrence of pathogenic free‐living amoeba and bacteria in 20 public indoor swimming pool facilities

et al. 2021

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Recently, indoor swimming pool activities have increased to promote health‐enhancing physical activities, which require establishing suitable protocols for disinfection and water quality control. Normally, the assessment of the microbial quality of the water in the pools only considers the presence of different bacteria. However, other less frequent but more resistant pathogens, such as free‐living amoebas (FLA), are not contemplated in both existing recommendation and research activities. FLA represent a relevant human health risk, not only due to their pathogenicity but also due to the ability to act as vehicles of other pathogens, such as bacteria. Therefore, this work aimed to study the physicochemical characteristics and the occurrence of potentially pathogenic FLA and bacteria in water samples from 20 public indoor swimming facilities in Northern Portugal. Our results showed that some swimming pools presented levels of pH, free chlorine, and conductivity out of the recommended limits. Pathogenic FLA species were detected in two of the facilities under study, where we also report the presence of both, FLA and pathogenic bacteria. Our findings evidence the need to assess the occurrence of FLA and their existence in the same environmental niche as pathogenic bacteria in swimming pool facilities worldwide and to establish recommendations to safeguard the health of the users.

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Isolation and molecular identification of free-living amoebae from dishcloths in Tenerife, Canary Islands, Spain

et al. 2019

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In vitro interactions of Acanthamoeba castellanii Neff and Vibrio harveyi

Cited by 6 publications

References 34 publications

Waterborne Isolates of Campylobacter jejuni Are Able to Develop Aerotolerance, Survive Exposure to Low Temperature, and Interact With Acanthamoeba polyphaga

Waterborne Isolates of Campylobacter jejuni Are Able to Develop Aerotolerance, Survive Exposure to Low Temperature, and Interact With Acanthamoeba polyphaga

Evaluation of the occurrence of pathogenic free‐living amoeba and bacteria in 20 public indoor swimming pool facilities

Isolation and molecular identification of free-living amoebae from dishcloths in Tenerife, Canary Islands, Spain

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