In vitro interactions between the defense systems of resistant and susceptible Biomphalaria alexandrina and sporocysts of Schistosoma mansoni

Abou-El-Naga, Iman F.; el-Nassery, S M; Allam, Sonia R.; Mady, Rasha Fadly

doi:10.1016/j.vetpar.2014.09.012

Cited by 4 publications

(7 citation statements)

References 26 publications

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“…While snails that remained uninfected after two exposures were isolated and reared singly for self-reproduction. Their progeny was the resistant stock [9] . From the two progenies, 60 susceptible and 60 resistant snails, sized about 8 mm, were selected to comprise the susceptible and the resistant groups, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Hemolymph collection: Snails shells were wiped with 70% alcohol and dried with an absorbent paper. Hemolymph collected by cardiac puncture was placed in a siliconized tube in an ice bath to prevent cellular aggregation and to permit the settling and removal of any debris [9] . Hemolymph collection was performed at 6 h, 1d, 3d, and 7d post individual exposure (PO) to 10 S. mansoni miracidia.…”

Section: Methodsmentioning

confidence: 99%

“…Conversely, granulocytes (population III) were found to be the most common cell type in the hemolymph of the susceptible (58.4±.4.1%) and resistant (61.9±0.75%) control snails. Intragroup comparisons for DHC are shown in figure (8) for populations, and in figure (9) for subpopulations.…”

Section: Differential Hemocyte Counts (Dhc)mentioning

confidence: 99%

“…Activation of the internal defense also occurs during parasite development inside susceptible snails, where, hemocytes temporarily bind to the parasites, allowing the development of sporocysts then cercariae. The latter are shed in freshwater to infect the definitive host [5,8,9] . Despite the considerable efforts spent on characterizing hemocytes in different mollusks, there are many controversies concerning their classification [10] .…”

Section: Introductionmentioning

confidence: 99%

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Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

Khedr

2020

Parasitologists United Journal

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Background: Few studies concerning Biomphalaria alexandrina (B. alexandrina) snail hemocytes' subpopulations, and their relation to the compatibility with Schistosoma mansoni (S. mansoni)' miracidia were performed. Manipulation of parasite development inside these snails could be applied as a control measure against schistosomiasis. Objectives: Knowing that the snail hemocytes temporarily bind to the parasites, allowing the development of cercariae that are infective to the definitive host. This study aimed at studying the morphology and kinetics of diverse hemocytes of susceptible and resistant B. alexandrina and their participation in the snail early immune response after challenge by S. mansoni. Material and Methods: Giemsa stained hemocytes were characterized using light microscopy. Total and differential hemocyte counts (THC and DHC) were calculated in the hemolymph of two groups composed of 60 susceptible and 60 resistant snails. Each group was further subdivided as 12 control pre-exposure snails (PE) and 48 post-exposure snails (PO) to S. mansoni at different time points (6 h, 1, 3 and 7 days). THC and DHC counts were recorded by a snail hemogram. Results: Results revealed that granulocytes constituted the most common population all through the experiment with the large dense-granulated granulocytes subpopulation being the largest-sized cells detected. The highly reactive subpopulations that increased in number upon exposure to S. mansoni were the few-granulated and the large-granulated granulocytes, suggesting their possible participation in early parasite destruction. Conclusion:The resulting hemograms helped determine the participation of hemocyte populations and subpopulations in the defense against S. mansoni, aiding in understanding snail compatibility patterns. Further studies to propagate transgenic B. alexandrina snails abundant in large granular granulocytes utilizing (gene editing) CRISPR-Cas9 technique are recommended. This would be required to spread schistosome resistance traits in snail populations, thus, contributing to reduced schistosomiasis transmission in the long run.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Differential Hemocyte Counts (Dhc)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

Khedr

2020

Parasitologists United Journal

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“…Molluscs have impressive capacities to protect themselves against infections caused by potential pathogens, which makes the resistance to infection the norm and susceptibility the exception (Bayne, 2009). This assumption explains why Biomphalaria species have different degrees of susceptibility with different internal defense system (IDS) and responses against the invasive S. mansoni (Abou-El-Naga, El-Nassery, Allam, & Mady, 2014). The snails are polymorphic regarding their compatibility with S.mansoni, with susceptible and resistant variants (Coelho et al, 2004).…”

mentioning

confidence: 99%

Total protein composition of young and adult Biomphalaria alexandrina snails with different compatibilities to Schistosoma mansoni infection

Sadaka

Abou-El-Naga

Amer

et al. 2016

RBT

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In the current study differentiation of the total proteins among Biomphalaria alexandrina snails at different ages was done to identify the proteins responsible for the snails’ compatibility outcome. The work was conducted on snails that differ in their age and the genetic backgrounds. Four subgroups from the progeny of self reproduced susceptible and resistant snails were studies. Infection rates of these subgroups (young susceptible, adult susceptible, young resistant and adult resistant) were 90%, 75%, 40% and 0% respectively. Using Sodium dodecyl sulphate polyacrylamide gel electrophoresis, differences in protein expression were shown between adult and young snails in different subgroups. Dice similarity coefficient was calculated to determine percentage band sharing among the experimental subgroups. The results revealed that the combination of similarities between both age and compatibility status of the snails has led to the highest similarity coefficient followed by the combination of similarities of both genetic origin and age although they differ in the compatibility status. On the other hand, the differences in the genetic background, age and compatibility status have led to the least similarity index. It was also found that in young snails the genetic background plays the main role in determination of their compatibility, while the internal defense system has the upper hand in determination of adult compatibility. Further characterization of the shared protein bands among the studied subgroups is needed to clarify their role in host-parasite relationship.

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Review: Schistosoma mansoni phosphatidylinositol 3 kinase (PI3K)/Akt/mechanistic target of rapamycin (mTOR) signaling pathway

Abou-El-Naga

2021

Comparative Biochemistry and Physiology Part B: Biochemistry an

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In vitro interactions between the defense systems of resistant and susceptible Biomphalaria alexandrina and sporocysts of Schistosoma mansoni

Cited by 4 publications

References 26 publications

Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

Total protein composition of young and adult Biomphalaria alexandrina snails with different compatibilities to Schistosoma mansoni infection

Review: Schistosoma mansoni phosphatidylinositol 3 kinase (PI3K)/Akt/mechanistic target of rapamycin (mTOR) signaling pathway

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