In vitro interactions between murine neutrophils and Eimeria falciformis sporozoites

Bekhti, Khadija; Kazanji, Mirdad; Pery, P.

doi:10.1016/0923-2494(92)80114-z

Cited by 13 publications

(2 citation statements)

References 26 publications

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“…In vivo, clearance of Brugia malayi microfilaria is very likely mediated through ADCC (203). ADCC activity against trypanosomes and other parasites has also been documented (204,205,206,207,208,209,210).…”

Section: Antibody-dependent Cellular Cytotoxicitymentioning

confidence: 99%

Functions of Antibodies

Forthal

2014

Microbiol Spectr

173

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Antibodies can impact pathogens in the presence or in the absence of effector cells or effector molecules such as complement, and experiments can often sort out with precision the mechanisms by which an antibody inhibits a pathogen in vitro. In addition, in vivo models, particularly those engineered to knock in or knock out effector cells or effector molecules, are excellent tools for understanding antibody functions. However, it is highly likely that multiple antibody functions occur simultaneously or sequentially in the presence of an infecting organism in vivo. The most critical incentive for measuring antibody functions is to provide a basis for vaccine development and for the development of therapeutic antibodies. In this respect, some functions, such as virus neutralization, serve to inhibit the acquisition of a pathogen or limit its pathogenesis. However, antibodies can also enhance replication or contribute to pathogenesis. This review emphasizes those antibody functions that are potentially beneficial to the host. In addition, this review will focus on the effects of antibodies on organisms themselves, rather than on the toxins the organisms may produce.

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Section: Antibody-dependent Cellular Cytotoxicitymentioning

confidence: 99%

Functions of Antibodies

Forthal

2014

Microbiol Spectr

173

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“…Neutrophils have been shown to play an important role in host immunity to a variety of pathogens including Mycobacterium avium (2), Chlamydia trachomatis (3), Candida albicans (16), Listeria monocytogenes (9,28), and Francisella tularensis (38) in vivo and E. falciformis (5) in vitro. Treating SCID mice with the antigranulocyte antibody significantly reduced the number of circulating neutrophils without significantly affecting the number of lymphocytes or monocytes.…”

Section: Discussionmentioning

confidence: 99%

Nonspecific immune responses and mechanisms of resistance to Eimeria papillata infections in mice

Schito

Barta

1997

Infect Immun

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Severe combined immunodeficient (SCID)-beige mice inoculated with the intracellular parasite Eimeria papillata produced significantly more oocysts during primary infections than inoculated immunodeficient SCID mice. Therefore, the addition of the beige mutation, which detrimentally affects neutrophil and natural killer (NK) cell functions, enhanced the parasites' ability to reproduce within the small intestine. To identify which of these two cell types is responsible for a protective immune response during primary infection, the following groups of mice were inoculated: (i) SCID mice depleted of neutrophils with antigranulocyte monoclonal antibody (RB6-8C5), (ii) C57BL/6 mice depleted of NK cells with the anti-NK-1.1 monoclonal antibody (PK136), and (iii) transgenic Tg26 ؉؉ mice (T and NK cell deficient). To identify the mechanisms of immunity during primary and secondary infections, gamma interferon (IFN-␥) knockout and perforin knockout mice were inoculated. Oocyst output was found to be significantly higher during primary infection for mice depleted of NK cells by administration of anti-NK-1.1 antibodies, for Tg26 ؉؉ mice, and for IFN-␥ knockout mice. During secondary infections, only perforin knockout mice produced significantly more oocysts compared to control mice. Our observations suggest that NK cells inhibit E. papillata oocyst output during primary infection by the production of IFN-␥ and that this inhibition is independent of perforin. Immunity to reinfection does not require IFN-␥ but appears to be mediated, at least in part, by a perforin-dependent mechanism.

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Production and characterization of monoclonal antibodies directed againstEimeria falciformis and cross-reactive with sporozoites from two species of avian coccidia

1994

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Monoclonal antibodies (mAbs) were obtained against the surface antigens of the Eimeria falciformis sporozoite by immunizing mice with whole homogenized sporozoite. The hybridomas were selected by their reactivities against oocyst extracts, then against glutaraldehyde-treated sporozoites. Three mAbs recognized both the surface of E. falciformis, E. tenella, and E. acervulina and their refractile bodies, whereas a fourth mAb recognized only one epitope on the refractile bodies. All mAbs bound to the same immunoaffinity-purified antigens in Western-blot analysis (P27 for E. falciformis and P25 for E. tenella and E. acervulina). Thus, the mAbs define at least two shared epitopes between sporozoite antigens from different eimerian species. Two of these mAbs are involved in the in vitro phagocytosis of E. falciformis sporozoites by macrophages and also in their lysis by neutrophils. Altogether, these properties showed that the four mAbs came from different activated B-cells. The P27 antigen recognized by our mAbs represents a major target of the in vitro destructive immune response.

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In vitro interactions between murine neutrophils and Eimeria falciformis sporozoites

Cited by 13 publications

References 26 publications

Functions of Antibodies

Functions of Antibodies

Nonspecific immune responses and mechanisms of resistance to Eimeria papillata infections in mice

Production and characterization of monoclonal antibodies directed againstEimeria falciformis and cross-reactive with sporozoites from two species of avian coccidia

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