In vitro Interaction of Metformin with Diclofenac in Aqueous Medium

Abstract

Combination therapy may be unavoidable and common way for the treatment of disease where two or more drugs are given concurrently. The drugs may exhibit effects independently or may interfere with each other. Metformin is an anti-diabetic drug and diclofenac is a NSAID. The in vitro interaction of metformin with diclofenac was studied at room temperature and at different pH conditions. The studies were performed by various UV-Visible spectrophotometric, conductometric and HPLC methods. It was found that metformin formed stable 1:1 complex with diclofenac. The interaction may greatly influence the activity of these molecules.

Does Rabeprazole Sodium Alleviate the Anti-diabetic Activity of Linagliptin? Drug-Drug Interaction Analysis by In vitro and In vivo Methods

“…The preliminary indication of the drug-drug interaction found from the obtained curves that were supported by Saha et al [13].…”

“…The technique is prevalently used in purification, identification, and quantification of individual components in the mix of compounds. The characteristic peak of the chromatogram and the R t value might be changed owing to interaction or molecular modification [13]. The R t value of the formed complex (5.303 min) was different than the two pure compounds that confirmed the formation of a new complex [29].…”

“…Preparation of 100 ml of 0.1 mM stock solutions [13] 47.25 mg of linagliptin or 38.24 mg of rabeprazole sodium was dissolved in DM water to prepare 0.1 mM stock solution of linagliptin and rabeprazole sodium, respectively. The volume was balanced up to 100 mL with the same solvent and finally held in the sonicator at room temperature for 10 min for proper dissolution.…”

“…Conductance is known as an inherent characteristic of an ion in the solution. The conductance property may be alternated if the substance interacts with other species in the solution [13]. The conductometric titration was used to get a primary indication of drugdrug interaction.…”

DSC and HPLC Studies of Some Common Antidiabetic and Antihypertensive Drugs

“…Drug-drug interactions also result an increase or a decrease in the action of either drugs, or it may produce an adverse effect that is not normally associated with the drugs (Hansten and Horn, 1989;Kristensen, 1976;Sangita et al, 2012;Saha et al, 2012). This particular interaction may be the result of a physicochemical incompatibility of the two drugs, or a change in the rate or quantity of absorption in the body, the binding ability of either drug, or an alteration in the ability of receptor sites and cell membranes to bind with either drug.…”