In-vitro-Inhibition von humanen Linsenepithelzellen (hLEZ) mittels eines pharmakologisch modifizierten Kapselspannringes zur Prävention der Cataracta secundaria

Kwittner, Sabine; Beck, Ruy Carlos Ruver; Nebe, Barbara

doi:10.1055/s-2007-976226

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2008

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“…Mibefradil containing micro particles were prepared by modifying a solvent evaporation procedure using ethyl acetate as dispersing solvent [23]. These micro capsules released the active agent with a daily average of 7.5 mg/l during a period of 50 days [24,25]. The micro particles were coupled on the PMMA (polymethyl methacrylate) surface of the capsular tension ring (Micromod Partikeltechnologie GmbH) [25] which contain 38 mg Mibefradil per gram.…”

Section: Materials and Methodologymentioning

confidence: 99%

Prevention of Lens Epithelial Cell Growth In Vitro Using Mibefradil-Containing PLGA Micro Particles~!2008-04-23~!2008-05-24~!2008-06-12~!

Weidmann¹,

Kwittner²,

Beck³

et al. 2008

TOOPHTJ

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The prevention of the posterior capsule opacification is still unsolved. To interfere with proliferating cells the T-type calcium channel antagonist Mibefradil was immobilized in poly-lactic-co-glycolic-acid micro particles which were fixed at a capsular tension ring and tested in a human organ culture model as well as in human lens cells HLE-B3 in vitro. It is feasible to get a release significantly affecting cell viability and growth evaluated by MTT test and cell cycle analysis. In addition, Bionas® sensor chips were used for time-dependent adhesion experiments in living lens cells. Interestingly, the concentration of Mibefradil which inhibited subconfluent cells is not effective in confluent cells. This is an important feature for the protection of the intact tissue in the eye.

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Section: Materials and Methodologymentioning

confidence: 99%