In vitro inhibition of the replication of haemorrhagic septicaemia virus (VHSV) and African swine fever virus (ASFV) by extracts from marine microalgae

Fábregas, Jaime; García, Digna; Fernandez‐Alonso, M.; Rocha, A.; Gómez‐Puertas, Paulino; Escribano, José M.; Otero, Ana; Coll, Julio

doi:10.1016/s0166-3542(99)00049-2

Cited by 119 publications

(51 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that C-phycocyanin from blue-green algae possesses antioxidant and anti-inflammatory properties Romay et al, 1999]. Extracts from some algae also have been demonstrated to have the antiviral activity [Carlucci et al, 1997;Ayehunie et al, 1998;Hoshino et al, 1998;Fabregas et al, 1999;Serkedjieva et al, 2000]. In this study, we examined the in vitro antienteroviral mechanism of allophycocyanin.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of enterovirus 71‐induced apoptosis by allophycocyanin isolated from a blue‐green alga spirulina platensis

Shih

Tsai

et al. 2003

Journal of Medical Virology

127

View full text Add to dashboard Cite

Enterovirus 71 infection causes significant morbidity and mortality in children, yet there is no effective treatment. In this study, a protein-bound pigment, allophycocyanin purified from blue-green algae is first reported to exhibit anti-enterovirus 71 activity. Allophycocyanin neutralized the enterovirus 71-induced cytopathic effect in both human rhabdomyosarcoma cells and African green monkey kidney cells. The 50% inhibitory concentration of allophycocyanin for neutralizing the enterovirus 71-induced cytopathic effect was approximately 0.045 +/- 0.012 microM in green monkey kidney cells. The cytotoxic concentrations of allophycocyanin for rhabdomyosarcoma cells and African green monkey kidney cells were 1.653 +/- 0.003 microM and 1.521 +/- 0.012 microM, respectively. A plaque reduction assay showed that the concentrations of allophycocyanin for reducing plaque formation by 50% were approximately 0.056 +/- 0.007 microM and 0.101 +/- 0.032 microM, when allophycocyanin were added at the state of viral adsorption and post-adsorption, respectively. Antiviral activity was more efficient in cultures treated with allophycocyanin before viral infection compared with that in the cultures treated after infection. Allophycocyanin was also able to delay viral RNA synthesis in the infected cells and to abate the apoptotic process in enterovirus 71-infected rhabdomyosarcoma cells with evidence of characteristic DNA fragmentation, decreasing membrane damage and declining cell sub-G1 phase. It is concluded that allophycocyanin possesses antiviral activity and has a potential for development as an anti-enterovirus 71 agent.

show abstract

Section: Introductionmentioning

confidence: 99%

Inhibition of enterovirus 71‐induced apoptosis by allophycocyanin isolated from a blue‐green alga spirulina platensis

Shih

Tsai

et al. 2003

Journal of Medical Virology

127

View full text Add to dashboard Cite

show abstract

“…Rechter et al (2006) also described spirulan-like molecules with pronounced antiviral activity in the absence of cytotoxic effects. Moreover, Fabregas et al (1999) screened the in vitro inhibition of viral replication of extracts obtained from several marine microalgae, indicating that the aqueous extracts from Porphyridium cruentum, Chorella autotrophica and Ellipsoidon sp. produced a significant inhibition of the in vitro replication of haemorrhagic septicemia virus and African swine fever virus.…”

Section: Introductionmentioning

confidence: 99%

Antiviral compounds obtained from microalgae commonly used as carotenoid sources

et al. 2011

View full text Add to dashboard Cite

Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoids sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pre-treatment of Vero cells with 75 g mL -1 of H. pluvialis ethanol extract inhibited virus infection by approx. 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50% respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour, since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication.The antiviral activity of water PLE extracts was found to correlate with polysaccharides, since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A GC-MS characterization of the H.pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid.

show abstract

“…Microalgal antiviral compounds might be taken as a food supplement, as some plant-based foods are good source of dietary antiviral molecules. Santoyo et al, 2012Ohta et al, 1998Santoyo et al, 2012Fábregas et al, 1999Lee et al, 2006Gustafson et al, 1989Gustafson et al, 1989 Anti-fungal Any biomolecule or chemical agent that selectively eliminates pathogenic fungal growth from the host. Microalgae with antifungal properties might be consumed as a food source, as that of several herbs consumed for keeping fungal diseases away.…”

Section: Referencesmentioning

confidence: 99%

Microalgae as a source of nutraceuticals

et al. 2015

View full text Add to dashboard Cite

In vitro inhibition of the replication of haemorrhagic septicaemia virus (VHSV) and African swine fever virus (ASFV) by extracts from marine microalgae

Cited by 119 publications

References 19 publications

Inhibition of enterovirus 71‐induced apoptosis by allophycocyanin isolated from a blue‐green alga spirulina platensis

Inhibition of enterovirus 71‐induced apoptosis by allophycocyanin isolated from a blue‐green alga spirulina platensis

Antiviral compounds obtained from microalgae commonly used as carotenoid sources

Microalgae as a source of nutraceuticals

Contact Info

Product

Resources

About