In vitro inhibition of rat basophilic leukaemia mast cell (RBL-2H3) degranulation by novel indane compounds

Passante, Egle; Ehrhardt, Carsten; Sheridan, Helen; Frankish, Neil

doi:10.1007/s00011-007-0607-1

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…They can also degranulate through a TLR2-dependent pathway [79]. Frankish and co-workers have previously shown [80] that RBL-2H3 are unresponsive to LPS, although TLR4 receptors are expressed on the cell surface [53]. This is inconsistent with previously published data where others have shown RBL-2H3 releasing up to 180 pg/ml of IL-13 and 60 ng/ml of TNF-a [81]; it is not clear why there should be such a conflict of data.…”

Section: Toll-like Receptorsmentioning

confidence: 99%

The RBL-2H3 cell line: its provenance and suitability as a model for the mast cell

2009

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The RBL-2H3 cell line is a commonly used histamine-releasing cell line used in inflammation, allergy and immunological research. Quite commonly, it is referred to in research papers as a mast cell line, despite the fact that it is derived from basophils. There is also a lack of consistency, both between different research groups using the same cell line and with both mast cell and basophil physiology. The review follows the development of the RBL-2H3 cell line from its inception and then goes on to assess the nature of the cell line in terms of its characteristics and its response to various stimuli. The relationship of this cell line to the various mast cell subtypes and basophils is discussed and it is concluded that while the RBL-2H3 cell line shares some characteristics with both mast cells and basophils, it is not fully representative of either.

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