In vitro inhibition of human cytomegalovirus replication in human foreskin fibroblasts and endothelial cells by ascorbic acid 2-phosphate

Činátl, Jaroslav; Weber, Bernard; Rabenau, Holger F.; Gümbel, H.; CHENOT, J; Scholz, Martin; Encke, A.

doi:10.1016/0166-3542(95)00024-g

Cited by 51 publications

(40 citation statements)

References 46 publications

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“…Virus stocks were prepared in HFF grown in MEM with 4% FBS. The respective titers were determined by plaque titration in HFF cells as described previously (21). Mock-infected inocula were prepared in an identical fashion, except that cell monolayers were not infected with HCMV.…”

Section: Virus Preparationmentioning

confidence: 99%

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Decreased Neutrophil Adhesion to Human Cytomegalovirus-Infected Retinal Pigment Epithelial Cells Is Mediated by Virus-Induced Up-Regulation of Fas Ligand Independent of Neutrophil Apoptosis

Činátl¹,

Blaheta

Bittoova³

et al. 2000

The Journal of Immunology

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Human CMV (HCMV) retinitis frequently leads to blindness in iatrogenically immunosuppressed patients and in the end stage of AIDS. Despite the general proinflammatory potential of HCMV, virus infection is associated with a rather mild cellular inflammatory response in the retina. To investigate this phenomenon, the influence of HCMV (strains AD169 or Hi91) infection on C-X-C chemokine secretion, ICAM-1 expression, and neutrophil recruitment in cultured human retinal pigment epithelial (RPE) cells was studied. Supernatants from infected cultures contained enhanced levels of IL-8 and melanoma growth-stimulating activity/Gro α and induced neutrophil chemotaxis compared with supernatants from uninfected RPE cells. Despite HCMV-induced ICAM-1 expression on RPE cells, binding of activated neutrophils to HCMV-infected RPE cells and subsequent transepithelial penetration were significantly reduced. Reduced neutrophil adhesion to infected RPE cells correlated with HCMV-induced up-regulation of constitutive Fas ligand (FasL) expression. Functional blocking of FasL on RPE cells with the neutralizing mAbs NOK-1 and NOK-2 or of the Fas receptor on neutrophils with mAbB-D29 prevented the HCMV-induced impairment of neutrophil/RPE interactions. Fas-FasL-dependent impairment of neutrophil binding had occurred by 10 min after neutrophil/RPE coculture without apoptotic signs. Neutrophil apoptosis was first detected after 4 h. Treatment of neutrophils with a specific inhibitor of caspase-8 suppressed apoptosis, whereas it did not prevent impaired neutrophil binding to infected RPE. The current results suggest a novel role for FasL in the RPE regulation of neutrophil binding. This may be an important feature of virus escape mechanisms and for sustaining the immune-privileged character of the retina during HCMV ocular infection.

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Section: Virus Preparationmentioning

confidence: 99%

“…HUVECs and human foreskin fibroblasts (HFF) were cultured as described previously (21). RPE cells were isolated from freshly enucleated bulbi for corneal transplantation of three donor eyes; the tenets of the Declaration of Helsinki were followed.…”

Section: Cell Culturementioning

confidence: 99%

Decreased Neutrophil Adhesion to Human Cytomegalovirus-Infected Retinal Pigment Epithelial Cells Is Mediated by Virus-Induced Up-Regulation of Fas Ligand Independent of Neutrophil Apoptosis

Činátl¹,

Blaheta

Bittoova³

et al. 2000

The Journal of Immunology

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“…All the ascorbic acid derivatives were characterized by 1 C NMR spectra were recorded on a Varian Gemini 300 spectrometer, operating at 75.46 MHz for the 13 C resonance. The samples were dissolved in DMSO-d 6 or CDCl 3 chemical shift values are in ppm, referred to TMS.…”

Section: B Methodsmentioning

confidence: 99%

“…Thus it protects cellular compounds against oxidative damage by free radicals and oxidants. [1] It is a vital nutrient for humans and has many important functions in the body. It plays a central role in the protection of cellular components against oxidative damage by free radicals and oxidants that are involved in the development and exacerbation of a multitude of chronic diseases such as cancer, heart disease, brain dysfunction, aging, rheumatism, inflammation, stroke, emphysema, and AIDS.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and In-Vitro Antioxidant Study Of 5, 6-O, O-Diacetyl-2, 3-O, O-Dibenzyl-L-Ascorbic Acid

Swain¹

2017

IJRASET

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“…It allows to collagen formation and stability, it also has other various physiological and therapeutic effects [80,[82][83][84][85][86][87][88] including the stimulation of human fibroblasts [82]. Interestingly, systemic application of high dose of L-AA inhibited tumor growth via reduction of angiogenesis [87,88].…”

Section: Ascorbic Acid Based Polyurethane Systems For Tementioning

confidence: 99%

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

Kucińska-Lipka¹,

St.²,

Janik³

et al. 2016

ChChT

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Abstract. The introduction of the paper was devoted to the main items of Tissue Engineering (TE) and the way of porous structure obtaining as scaffolds. Furthermore, the significant role of the scaffold design in TE was described. It was shown, that properly designed polyurethanes (PURs) find application in TE due to the proper physicochemical, mechanical and biological properties. Then the use of L-ascorbic acid (L-AA) in PUR systems for TE was described. L-AA has been applied in this area due to its suitable biological characteristics and antioxidative properties. Moreover, L-AA influences tissue regeneration due to improving collagen synthesis, which is a primary component of the extracellular matrix (ECM). Modification of PUR with L-AA leads to the materials with higher biocompatibility and such system is promising for TE applications.

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In vitro inhibition of human cytomegalovirus replication in human foreskin fibroblasts and endothelial cells by ascorbic acid 2-phosphate

Cited by 51 publications

References 46 publications

Decreased Neutrophil Adhesion to Human Cytomegalovirus-Infected Retinal Pigment Epithelial Cells Is Mediated by Virus-Induced Up-Regulation of Fas Ligand Independent of Neutrophil Apoptosis

Decreased Neutrophil Adhesion to Human Cytomegalovirus-Infected Retinal Pigment Epithelial Cells Is Mediated by Virus-Induced Up-Regulation of Fas Ligand Independent of Neutrophil Apoptosis

Synthesis and In-Vitro Antioxidant Study Of 5, 6-O, O-Diacetyl-2, 3-O, O-Dibenzyl-L-Ascorbic Acid

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

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