In vitro inhibition of bacteria against Fusarium oxysporum f. sp. cubense race 2

Abstract

There are three races of Fusarium oxysporum f. sp. cubense (Foc) that affect bananas and plantains, though the Cavendish group banana cultivars are resistant to Foc race 1 (FocR1) and Foc race 2 (FocR2). However, there are no effective control methods for the Tropical Race 4 (R4T). The foregoing makes it necessary to generate biological control strategies for FocR2 fungal model that is present in Mexico, forecasting the potential introduction of FocR4T into our country. In vitro inhibition was evaluated of bacterial strains Bacillus subtilis (BASU), B. velezensis (BAVE), and B. sonorensis (BASO) against three isolates of FocR2 (MIC17188, MIC17191 and MIC17192), obtained from plant samples collected in three banana production sites in the states of Puebla, Michoacán and Oaxaca. At 14 days, the strain BASU had in average high inhibition (55.1%) in comparison to BAVE and BASO strains (45.8 and 21.7%, respectively) against the three fungal strains mentioned before. In addition, the in vitro inhibition of Paenibacillus sp. (BSP 1.1) and Serratia sp. (AC35) was also evaluated by considering three pre-inoculation timings; the strain BSP1.1 showed high inhibition (80.1%) when pre-inoculated individually seven days before the fungus and at seven days of confrontation.

Application of Biplot Techniques to Evaluate the Potential of Trichoderma spp. as a Biological Control of Moniliasis in Ecuadorian Cacao

“…containing 7 days of mycelial growth and discs of the same length of the pathogenic fungus such as Moniliophthora roreri (MRCP) and Moniliophtora roreri (MMCA) with 4 days of development were placed at the opposite side. The linear growth monitoring of the two opposing colonies was recorded twice daily until the complete colonization of the Petri dish [20].…”