In vitro inhibition of adhesion of enterotoxigenic Escherichia coli K88 to piglet intestinal mucus by egg-yolk antibodies

Jin, L

doi:10.1016/s0928-8244(98)00086-8

Cited by 37 publications

(66 citation statements)

References 23 publications

(44 reference statements)

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“…The isolate, which was obtained from the feces of a piglet suffering from diarrhea, was shown to give a positive agglutination test with anti-K88 ETEC antibody but not with anti-K99 ETEC antibody. E. coli K88ϩMB was tested as the K88ac phenotype (16). Primary cultures of ETEC strains were grown overnight in tryptic soy broth (Difco) at 37°C using a 1% innoculum from stocks stored at Ϫ20°C.…”

Section: Methodsmentioning

confidence: 99%

“…The piglets used in this study were of the K88-susceptible phenotype, since K88-bearing E. coli MB was able to bind to the intestinal epithelial cells when tested microscopically using the procedure described by Wilson and Hohmann (27). The small intestine mucus used in the present study was isolated from piglets by the procedure of Jin et al (16). Briefly, a microscope slide was used to gently scrape the mucosal surface into HEPESHanks' buffer (HH buffer; pH 7.4).…”

mentioning

confidence: 99%

“…The assay of inhibition of adhesion was similar to that carried out previously (16). The E. faecium culture was prepared by incubation in KF Streptococcus broth for 25 h at 37°C.…”

mentioning

confidence: 99%

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A Strain of Enterococcus faecium (18C23) Inhibits Adhesion of Enterotoxigenic Escherichia coli K88 to Porcine Small Intestine Mucus

Jin

Marquardt

Zhao

2000

Appl Environ Microbiol

105

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Few studies, if any, have addressed the adhesion of enterococci to the intestinal mucosa and their interference with the adhesion of pathogens, although more than 60% of probiotic preparations in the market contain strains of enterococci. The objective of this study was to investigate if Enterococcus faecium 18C23 has the ability to inhibit the adhesion of Escherichia coli K88ac and K88MB to the small intestine mucus of piglets. Approximately 9% of E. faecium 18C23 organisms adhered to the small intestine mucus, and the adhesion was found to be specific. Living E. faecium 18C23 culture efficiently inhibited the adhesion of E. coli K88ac and K88MB to the piglet intestine mucus. Inhibition of the adhesion of E. coli K88ac to the small intestine mucus was found to be dose dependent. Inhibition of >90% was observed when 10 9 CFU or more of living E. faecium 18C23 culture per ml was added simultaneously with E. coli to immobilized mucus. The substances from both the 18C23 cells and the spent culture supernatant contributed to the inhibition of adhesion of E. coli K88 to the small intestine mucus receptors. The inhibiting effect was not solely a pH effect since considerable inhibitory action was demonstrated after neutralizing the mixture or spent culture supernatant to pH 7.0. Part of the inhibition of adhesion of E. coli K88ac by E. faecium 18C23 or its supernatant might occur through steric hindrance.Enterotoxigenic Escherichia coli (ETEC) strains are frequent causes of piglet diarrhea during the preweaning and immediate postweaning periods. Among the different ETEC strains (K88-, K99-, or 987P-expressing strains), those expressing K88 fimbrial antigen are the most prevalent (1, 13). These fimbriae mediate the adhesion of E. coli K88-expressing strains to the intestinal epithelial mucosa and also to the mucus layer lining the small intestine (7), and thereafter the organism elaborates one or two enterotoxins, heat-stable toxin and heatlabile toxin, which induce massive fluid and electrolyte secretion into the gut lumen (1, 13). Among ETEC variants expressing the ab, ac, or ad K88 fimbriae, those possessing the K88ac fimbrial antigen are the most common variant found in pathogenic E. coli isolates in the United States (26). Antibiotics are routinely used in an attempt to control pathogens, but the organisms are becoming resistant to the more commonly used treatments, making antibiotic therapy unreliable (10). Furthermore, the use of antimicrobial growth promoters may cause the development of resistance in a number of important pathogenic bacterial species (28). Recently, the European Union decided to ban the use of four widely used antibiotics, i.e., tylosin, virginiamycin, spiramycin, and zinc bacitracin, as growth promoters from July 1999. As a consequence, there is an urgent need to seek an alternative to antibiotics for the purpose of enhancing the health status and production performance of domestic animals.Probiotics have been used to reduce the colonization of the intestines of animals by pathogens. This, in ...

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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A Strain of Enterococcus faecium (18C23) Inhibits Adhesion of Enterotoxigenic Escherichia coli K88 to Porcine Small Intestine Mucus

Jin

Marquardt

Zhao

2000

Appl Environ Microbiol

105

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“…IgYs can be generated against colonization factors (outer membrane proteins, fimbriae/pili and lipopolysaccharides), flagella, mucosal receptors, enzymes and toxins important for bacterial survival [40,41].…”

Section: Specific Igy Antibodies Against Pathogens-structure and Funcmentioning

confidence: 99%

IgYs as an Alternative Approach to Antibiotics

Zajac¹,

Schubert²,

Oelkrug³

2017

J Antimicrob Agents

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Misuse and overdose of antibiotics in agriculture and human disease lead to a worldwide problem of resistant bacteria. In 2012, 10,000 metric tons of antibiotics in the US and 8,000 in Europe were used in animals for the food industry. Resistance can be transferred within and between bacteria species, but also spread easily over to humans by contact with contaminated food, soil or infected animals and patients. To find a successful therapy against resistant microbes is extremely difficult due to the high cost of developing next generation antibiotics and its production, but also because of bacteria ability of developing new mechanisms against these drugs. Therefore, technology based on avian antibodies IgYs can be a strong alternative for antibiotic therapy. Advantages of IgYs include: low costs, effective and non-harmful method of production. But the strongest argument is that they cannot be affected by bacterial resistance. IgYs can be used not only as a preventative or therapeutic but also as growth promoters in food production. In this review we present the reasons for antibiotic resistance in bacteria and its consequences in the field of farming and human medicine, including molecular biology mechanisms and statistics. Furthermore we introduce IgYs as a promising and potential candidate for the replacement of antibiotics in the near future.

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“…Feeding the yolk to pigs provides passive immunity to the target disease. Based on this concept, many researches were focused on IgY to prevent E. coli pathogens because diarrhea in neonatal and postweaning pigs is a significant and persistent problem in pig production and among the major causes of diarrhea in piglets are enterotoxigenic E. coli (ETEC) strains expressing K88, K99 and 987P fimbrial antigens (Jin et al, 1998). Several studies showed immuned egg yolk reduces adherence and colonization of ETEC strains, mortality, clinical signs, or diarrhea score in neonatal pigs (Imberechts et al, 1997;Marquardt et al, 1999;Owusu-Asiedu et al, 2002;Zuniga et al, 1997).…”

Section: Dried Eggmentioning

confidence: 99%

Value of spray-dried egg in pig nursery diets

Song¹,

Kim²,

Kim³

et al. 2015

Korean Journal of Agricultural Science

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: High-quality protein ingredients have been used in nursery diets, in spite of expensive ingredients, to minimize nutritional deficiency and disease problems. Recent dramatic increases in prices of protein products for nursery diets have exacerbated the challenge. Spray-dried egg may be a part of the solutions. Therefore, this review describes the value of spray-dried egg in nursery diets as a high-quality protein source. Spray-dried egg is egg by-product and is produced by only eggs without shell that are below the USDA Grade B standards. Spray-dried egg is an excellent nutrient source: 1) highly digestible, 2) excellent balance of amino acids, 3) rich content of fat, and 4) high metabolizable energy. These can be attributed to growth of nursery pigs. Beyond the provision of bioavailable nutrients, spray-dried egg also may provide specific physiological benefits. Spray-dried egg contains 1) immunoglobulin antibodies (IgY: IgG in egg yolk) that may attach to intestinal pathogens and excrete them and 2) lysozymes antimicrobial protein that can damage bacteria cell wall. Thereby feeding spray-dried egg may reduce concentration of intestinal pathogen and thus improve potential gut health or enteric disease resistance in nursery pigs. This is important for physiologically immature weaned pigs. Based on these benefits, spray-dried egg is believed to have the same benefits as spray-dried plasma protein and milk products in diets for nursery pigs. Therefore, it is suggested that spray-dried egg has a great potential as a valuable protein source in nursery diets.

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In vitro inhibition of adhesion of enterotoxigenic Escherichia coli K88 to piglet intestinal mucus by egg-yolk antibodies

Cited by 37 publications

References 23 publications

A Strain of Enterococcus faecium (18C23) Inhibits Adhesion of Enterotoxigenic Escherichia coli K88 to Porcine Small Intestine Mucus

A Strain of Enterococcus faecium (18C23) Inhibits Adhesion of Enterotoxigenic Escherichia coli K88 to Porcine Small Intestine Mucus

IgYs as an Alternative Approach to Antibiotics

Value of spray-dried egg in pig nursery diets

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