In vitro influence of Phaseolus vulgaris, Griffonia simplicifolia, concanavalin A, wheat germ, and peanut agglutinins on HCT-15, LoVo, and SW837 human colorectal cancer cell growth.

Kiss, Róbert; Camby, Isabelle; Duckworth, Carrie A.; Decker, Robert De; Salmon, Isabelle; Pasteels, Jean-Lambert; Danguy, André; Yeaton, Paul

doi:10.1136/gut.40.2.253

Cited by 51 publications

(41 citation statements)

References 21 publications

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“…This effect was also found by Kiss et al 1997 with 3 different human colorectal cancer cell lines and by Lorea et al 1997 for PNA with melanoma cells. Camby et al 1997 found a weak stimulatory effect of PHA-L with astrocytic tumor cell lines during 48-96 hour incubations.…”

Section: Discussionsupporting

confidence: 52%

“…Some of these lectins have demonstrated toxicity, to varying degrees, against some cancer cell lines in vitro, including: colon Kiss et al, 1997), breast (Valentiner et al, 2003), melanoma (Lorea et al, 1997;Litynska et al, 2001) and pancreatic cancers (Schwarz et al, 1999). These lectins have the potential to be used to target cancer cells for death.…”

Section: Discussionmentioning

confidence: 99%

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Direct targeting of cancer cells: A multiparameter approach

et al. 2005

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SUMMARYLectins have been widely used in cell surface studies and in the development of potential anti-cancer drugs. Many past studies that have examined lectin toxicity have only evaluated the effects on cancer cells, not their non-cancer counterparts. In addition, few past studies have evaluated the relationship between lectin-cell binding and lectin toxicity on both cell types. Here we examine these parameters in one study: lectin-cell binding and lectin toxicity with both cancer cells and their normal counterparts. We found that the human colon cancer cell line CCL-220/Colo320DM bound to agarose beads derivatized with Phaseolus vulgaris agglutinin (PHA-L) and wheat germ agglutinin (WGA), while the non-cancer human colon cell line CRL-1459/CCD-18Co did not. When these lectins were tested for their effects on cell viability in culture, both cell lines were affected by the lectins but at 6, 48 and 72 hours incubation times, PHA-L was most toxic to the cancer cell line in a concentration dependent manner. At 48 hours incubation, WGA was more toxic to the cancer cell line. The results suggest that it may be possible to develop lectin protocols that selectively target cancer cells for death. In any case, examination of both malignant cells and their non-malignant counterparts, analysis of their binding characteristics to immobilized lectins, and examination of the toxicity of free lectins in culture, provides a multiparameter model for obtaining more comprehensive information than from more limited approaches. KeywordsImmobilized lectin binding; lectin toxicity in culture; human colon cancer and non-cancer colon cells; PHA-L; WGA

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Direct targeting of cancer cells: A multiparameter approach

et al. 2005

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“…Other studies also suggest that a wide range of concentrations of agents should be initially tested for their effects on cells. Sano et al (2000) found that migration of monocytes was best stimulated at 3μM galectin-3 while less stimulation occurred at 10μM or 0.01μM, while Kiss et al (1997) found lectin induced growth stimulation and lectin induced growth inhibition at different concentrations. They also found greater effects of a lectin on growth stimulation at low concentrations with no effect at high concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…A large number of studies with PNA indicate that this lectin acts in a cell type specific manner, often promoting proliferation and sometimes producing a toxic effect (Lee 1988;Caldero et al, 1989;McGarrity et al, 1991;Boland et al, 1992;Ryder et al, 1992;Chen et al, 1994;Kiss et al, 1997;Lorea et al, 1997;Jordinson et al, 1998;Yu et al, 2001;Singh et al, 2001;2006;Ohba et al, 2003;Cai et al, 2005). Valentiner et al (2003) in a model study showed that different lectins exerted different effects at specific concentrations on different breast cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…In the study reported here, we examine two lectins that are reported to be largely non-toxic to cells -Dolichos biflorus agglutinin (DBA) and Arachis hypogaea /peanut agglutinin (PNA) (Ryder et al, 1992;Chen et al, 1994;Camby et al, 1997;Kiss et al, 1997;Jordinson et al, 1998;Ohba et al, 2003;Valentiner et al, 2003;Singh et al, 2006) -using a human colon cancer (CCL-220) and a human non-cancer colon CRL-1459) cell line, as in our previous studies (Khurrum et al, 2002;Heinrich et al, 2005;Welty et al, 2006). We did not want to use toxic lectins that kill cells quickly.…”

Section: Introductionmentioning

confidence: 99%

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Lectin binding and effects in culture on human cancer and non-cancer cell lines: Examination of issues of interest in drug design strategies

2007

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SummaryBy using a non-cancer and a cancer cell line originally from the same tissue (colon), coupled with testing lectins for cell binding and for their effects on these cell lines in culture, this study decribes a simple multiparameter approach that has revealed some interesting results that could be useful in drug development strategies.Two human cell lines, CCL-220/Colo320DM (human colon cancer cells, tumorigenic in nude mice) and CRL-1459/CCD-18Co (non-malignant human colon cells) were tested for their ability to bind to agarose microbeads derivatized with two lectins, peanut agglutinin (Arachis hypogaea agglutinin, PNA) and Dolichos biflorus agglutinin (DBA), and the effects of these lectins were assessed in culture using the MTT assay.Both cell lines bound to DBA-derivatized microbeads, and binding was inhibited by N-acetyl-Dgalactosamine, but not by L-fucose. Neither cell line bound to PNA-derivatized microbeads. Despite the lack of lectin binding using the rapid microbead method, PNA was mitogenic in culture at some time points and its mitogenic effect displayed a reverse-dose response. This was also seen with effects of DBA on cells in culture.

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Antitumor effect of plant lectins

Abdullaev

Mejı́a

1997

Natural Toxins

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This review examines the literature data concerning the biological activity of plant lectins. Numerous studies have reported that these substances possess toxic, cytotoxic, antitumor, and anticarcinogenic properties. A brief description of the biological properties of plant lectins, as well as the effect of plant lectins on normal and malignant cells and the antitumor properties of these lectins in vivo and in vitro, are included. These findings are interpreted and possible mechanisms of the antitumor effect of plant lectins are discussed. Nat.

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In vitro influence of Phaseolus vulgaris, Griffonia simplicifolia, concanavalin A, wheat germ, and peanut agglutinins on HCT-15, LoVo, and SW837 human colorectal cancer cell growth.

Abstract: Background/Aims-Compared with nor-

Cited by 51 publications

References 21 publications

Direct targeting of cancer cells: A multiparameter approach

Direct targeting of cancer cells: A multiparameter approach

Lectin binding and effects in culture on human cancer and non-cancer cell lines: Examination of issues of interest in drug design strategies

Antitumor effect of plant lectins

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