In vitro influence of gastrin, oestradiol and gonadotropin-releasing hormone on HCT-15 and LoVo human colorectal neoplastic cell proliferation

Kiss, Róbert; Salmon, Isabelle; Pauwels, Olivier S. G.; Gras, Stephan; Danguy, André; Etiévant, Chantal; Pasteels, Jean-Lambert; Martinez, Jean

doi:10.1016/0277-5379(91)90095-u

Cited by 12 publications

(5 citation statements)

References 18 publications

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“…While they are original in the case of thyroid tissues, the LHRH-mediated eects on thyroid cell proliferation are not surprising since it has been known for many years that LHRH is able to modify cell proliferation rates directly in various tumor tissue types, including those of the colon (Kiss et al 1991;Darro et al 1995), the prostate (Dondi et al 1996;Qayum et al 1990), the breast (Segal-Abramson et al 1992;Miller et al 1985) and the ovary (Yano et al 1994).…”

Section: Discussionmentioning

confidence: 95%

“…In addition, the human thyroid gland appears to have the potential for both estrogen synthesis and intracrine or paracrine estrogen responsiveness, all of which seem to be greater in women than men and may become enhanced with the process of tumorigenesis (Dalla Valle et al 1998). As elsewhere Rehfeld and van Solinge 1994), in our laboratory (Camby et al 1996;Darro et al 1995;Kiss et al 1991) we are interested in the role that the luteinizing-hormone-releasing hormone (LHRH) and gastrin could exert on cell proliferation activity in various tumor types. These LHRH-and gastrin-mediated roles in thyroid tissues have not been investigated so far.…”

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confidence: 99%

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The influence of l -triiodothyronine, l -thyroxine, estradiol-17β, the luteinizing-hormone-releasing hormone, the epidermal growth factor and gastrin on cell proliferation in organ cultures of 35 benign and 13 malignant human thyroid tumors

Nagy

Camby

Decaestecker

et al. 1999

Journal of Cancer Research and Clinical Oncology

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Triiodothyronine, thyroxine, LHRH and gastrin may increase or decrease cell proliferation in human thyroid tissues, whether benign or malignant, to the same extent as other hormones and/or growth factors such as thyrotropin, EGF, insulin-like growth factor 1, transforming growth factor beta1 and estradiol the effects of which on thyroid cell proliferation are already well documented in the literature.

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Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

The influence of l -triiodothyronine, l -thyroxine, estradiol-17β, the luteinizing-hormone-releasing hormone, the epidermal growth factor and gastrin on cell proliferation in organ cultures of 35 benign and 13 malignant human thyroid tumors

Nagy

Camby

Decaestecker

et al. 1999

Journal of Cancer Research and Clinical Oncology

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“…Growth Assessments Cell growth was assessed by means of the MTT assay as reported previously (12,19). Briefly, after the incubation of the cells for 96hrs i n the 11 different media (passage P91), the culture medium was removed and replaced with 100 pl MTT (Sigma) a t 1 mg/ml RPMI medium (Seromed, Germany).…”

Section: Experimental Schedule For Cellmentioning

confidence: 99%

“…The medium was replaced with 100 p1 dimethylsulphoxide (DMSO). The multiwells were shaken on a plate-shaker for 10 min; they were then read on a BioTek Instrument Microplate Reader (EL 308) using a test wavelength of 570 nm and a reference one of 630 nm (12,19). The 11 variant cells from the parent HCT-15 and LOVO cell lines were incubated in a hormone-free medium for 24 h to ensure good plating conditions.…”

Section: Experimental Schedule For Cellmentioning

confidence: 99%

“…In describing the concepts of in vitro "pseudo-tissular" differentiation, our present study came up against two tests that enabled cell proliferation to be assessed. These were the MTT (3-[4,5-dimethylthiazol-2yL] -2,5 -diphenytetrazolium bromide) test, which is a simple colorimetric assay enabling the growth level of cell lines to be determined (12,19), and a test based on the digital cell image analysis of Feulgen-stained nuclei (10,16) which allowed the assessment of the proliferation index, i.e., the percentage of cells in the S phase of the cell cycle.…”

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Characterization of the differentiation of human colorectal cancer cell lines by means of Voronoi diagrams

et al. 1993

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This paper describes differentiation in terms of population dynamics through the medium of Voronoi paving which enables (via digital cell image analysis) the structure of human LOVO and HCT-15 colorectal neoplastic cell colonies growing on histological slides to be characterized. Two other tests were also used, i.e., the colorimetric MTT assay that enables the cell growth level to be determined, and a test allowing the assessment of the proliferation index, i.e., the percentage of cells in the S phase of the cell cycle. The results show that these colorectal neoplastic cells exhibited a comparatively high level of organisation in terms of the topographical distribution of nuclei within the clones when the cells were cultivated in media containing even small amounts of fetal calf serum. On the other hand, certain chemically defined media completely overturned this "pseudo-tissular" architecture. Furthermore, the colorectal cells growing in media including fetal calf serum exhibited relatively large and dense clones, undergoing an increase in the density of these clones when hormones were added to the culture medium and, concomitantly, a decrease in their proliferation. In contrast, the cells growing in chemically defined media generally exhibited smaller clones whose cell proliferation was paradoxically greater than that of the cells referred to above. This seems to bring out the importance of the part played by the cell loss factor in this cell population dynamic.

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The in vitro influence of eight hormones and growth factors on the proliferation of eight sarcoma cell lines

Remmelink

Decaestecker

Darro

et al. 1998

Journal of Cancer Research and Clinical Oncology

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Little is known about the regulation of sarcoma proliferation by hormones and/or growth factors. We therefore characterised the in vitro proliferative influence on eight sarcoma cell lines of the platelet-derived growth factor, the insulin-like growth factor 1, triiodothyronine, the epidermal growth factor, the luteinising-hormone-releasing hormone, progesterone, gastrin and 17 beta-oestradiol. The influence of the different factors on the proliferation of sarcoma cell lines was measured by the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Two culture media were studied: (1) a nutritionally poor medium containing 2% of fetal calf serum and (2) a nutritionally rich one containing 5% or 10% FCS both with and without the addition of non-essential amino acids. The results were analysed either by conventional statistical analyses or by a classification method based on a decision-tree approach developed in Machine Learning. This latter method was also compared to other classifiers (such as logistic regression and k nearest neighbours) with respect to its accuracy of classification. Monovariate statistical analysis showed that each of the eight cell lines exhibited sensitivity to at least one factor, and each factor significantly modified the proliferation of five or six of the eight cell lines under study. Of these eight lines one of fibrosarcoma origin was the most "factor-sensitive". Decision-tree-related data analysis enabled the specific pattern of factor sensitivity to be characterised for the three histological types of cell line under study. The effects of hormone and growth factors are significantly influenced by the type of culture medium used. The method used appeared to be an accurate classifier for the kind of data analysed. Sarcoma proliferation can be modulated, at least in vitro, by various hormones and growth factors, and the proliferation of each histopathological type exhibited a distinct sensitivity to different hormone and/or growth-factors.

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In vitro influence of gastrin, oestradiol and gonadotropin-releasing hormone on HCT-15 and LoVo human colorectal neoplastic cell proliferation

Cited by 12 publications

References 18 publications

The influence of l -triiodothyronine, l -thyroxine, estradiol-17β, the luteinizing-hormone-releasing hormone, the epidermal growth factor and gastrin on cell proliferation in organ cultures of 35 benign and 13 malignant human thyroid tumors

The influence of l -triiodothyronine, l -thyroxine, estradiol-17β, the luteinizing-hormone-releasing hormone, the epidermal growth factor and gastrin on cell proliferation in organ cultures of 35 benign and 13 malignant human thyroid tumors

Characterization of the differentiation of human colorectal cancer cell lines by means of Voronoi diagrams

The in vitro influence of eight hormones and growth factors on the proliferation of eight sarcoma cell lines

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