In vitro induction of tetraploid Ziziphus jujuba Mill. var. spinosa plants from leaf explants

Cui, Yanhong; Hou, Lei; Li, Xiang; Huang, Feiyi; Pang, Xiaoming; Li, Yingyue

doi:10.1007/s11240-017-1274-8

Cited by 37 publications

(38 citation statements)

References 45 publications

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“…These changes may provide polyploids with short-term adaptive potential (Van de Peer et al, 2017) and the opportunity to exploit new niches (e.g., Marchant et al, 2016). In addition, synthetic polyploids have been largely employed to increase agronomic traits mainly due to its higher physiological and morphological fitness compared to their natural diploids (Chung et al, 2017;Cui et al, 2017;Salma et al, 2017;Wei et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

et al. 2020

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Polyploidy is widely recognized as a major evolutionary force in plants and has been reported in the genus Lippia (Verbenaceae). Lippia alba, the most studied species, has been documented as a polyploid complex involving at least four ploidal levels. L. alba presents remarkable chemical and genetic variation and represents a model for understanding genome organization. Although the economic and medicinal importance of the species has been widely described, no established polyploid induction protocol has been reported so far. Here, we describe the production of synthetic polyploid plants of L. alba using colchicine. The ploidal levels were estimated by flow cytometry and chromosome counting. In addition, FISH and molecular markers approaches were used to confirm the stability of the synthetic polyploids. The major component of the essential oils was estimated by GCMS to compare with the natural individuals. Tetraploids and triploids were produced providing new opportunities for investigating medicinal, pharmacological, and economic applications as well as addressing intrinsic questions involved in the polyploidization process in tropical plants.

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Section: Introductionmentioning

confidence: 99%

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

et al. 2020

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“…Colchicine is a highly effective mitotic spindle inhibitor that has been used with great success to improve the agronomic traits of a number of species via polyploid induction [ 4 , 6 , 22 ]. In this study, we investigated the effects of exposure time and colchicine concentration on tetraploid induction.…”

Section: Resultsmentioning

confidence: 99%

“…Relatively mature leaves of diploid and tetraploid ex vitro plants were used to compare differences in stomata. Several excised pieces of epidermal layer from the abaxial side of leaves were mounted on glass slides with a drop of distilled water and covered with a cover slip [ 6 , 22 ]. The density of stomata was measured under a light microscope under a 20× objective lens, with 30 randomly selected microscopic fields per sample.…”

Section: Methodsmentioning

confidence: 99%

In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)

et al. 2020

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Artificial induction of polyploidy is widely used in breeding programmes to improve the agronomic traits. The drumstick tree (Moringa oleifera Lam.) has a range of potential commercial uses, as the vegetative organs have high nutritional, medicinal, and feed values. In the present study, in vitro tetraploidisation in drumstick tree was performed by treating leaf segments with colchicine and subsequently verifying the ploidy levels. For polyploidisation, explant survival and regeneration rates were affected more by exposure time than by colchicine concentration, and the highest polyploidisation efficiency was observed at 500 mg/L colchicine for 3 days, which yielded 21% tetraploids. The morphological characteristics and contents of seven fodder-related nutrients (crude protein, ether extract, ash, acid detergent fibre, neutral detergent fibre, calcium, and phosphorus) in the leaves and shoots were compared between tetraploid and diploid drumstick trees. The resulting tetraploids showed significantly enhanced leaf and stomatal size. In addition, the contents of seven fodder-related nutrients were higher, although to varying degrees, in tetraploids than in diploids. The results indicated that the tetraploid produced in this study exhibited superior agronomical traits and improved biomass yield than diploids, and may represent excellent raw materials for fodder to enhance biomass and nutrition.

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“…The contents of vitamin C, cAMP, soluble sugars, titratable acids, sucrose, glucose, and fructose were significantly higher in tetraploid 'Riguang' fruits than in diploid 'Dongzao' fruits 79 . It was discovered that an autotetraploid sour jujube had higher tolerance to salinity than the diploid, and its preliminary molecular mechanism was illustrated 80,81 . In addition, triploids of 'Jinsixiaozao', 'Yuanlingzao', and 'Changhongzao' have been created by endosperm culture, but no new cultivar has been released 82 .…”

Section: Creating a Mixoploid-free Polyploid Induction Systemmentioning

confidence: 99%

The historical and current research progress on jujube–a superfruit for the future

et al. 2020

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Jujube (Ziziphus jujuba Mill.), or Chinese date, is the most important species of Rhamnaceae, a large cosmopolitan family, and is one of the oldest cultivated fruit trees in the world. It originates from the middle and lower reaches of the Yellow River, the 'mother river' of the Chinese people. It is distributed in at least 48 countries on all continents except Antarctica and is becoming increasingly important, especially in arid and semiarid marginal lands. Based on a systematic analysis of the unique characteristics of jujube, we suggest that it deserves to be recognized as a superfruit. We summarized historical research achievements from the past 3000 years and reviewed recent research advances since 1949 in seven fields, including genome sequencing and application, germplasm resources and systematic taxonomy, breeding and genetics, cultivation theory and techniques, pest control, postharvest physiology and techniques, and nutrition and processing. Based on the challenges facing the jujube industry, we discuss eight research aspects to be focused on in the future.

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In vitro induction of tetraploid Ziziphus jujuba Mill. var. spinosa plants from leaf explants

Cited by 37 publications

References 45 publications

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)

The historical and current research progress on jujube–a superfruit for the future

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