In-vitro impacts of Triladyl extender on semen quality parameters of Tharparkar bull

Abstract

This study was carried out to see the In-vitro impacts of Triladyl  extender on cooled and frozenthawed semen quality parameters of Tharparkar Bull, for four mature bulls (labeled as A, B, C, and D), all aged between 4 to 5 years were used. A total of 60 (n=15) semen samples were collected by the artificial vagina method and kept in a warm water bath at 37⁰C. After collection, fresh semen ejaculates of individual bulls were evaluated for macroscopic (Volume, pH, Colour) and microscopic (Motility, Live dead ratio, Morphology, HOST and concentration) parameters. Semen ejaculates qualifying the standard criteria were pooled and extended in Triladyl  extender. Evaluation of cooled semen motility (73±1.04), live-dead ratio (79.25±0.96), morphology (79.35±0.91) and HOST (66.975±1.067) with Triladyl  extenders showed improved results. Evaluation of frozen-thawed semen motility (51±0.785), live dead ratio (68.625±0.885), morphology (68.55±0.872) and HOST (48.225±0.87) by employing Triladyl  extenders showed satisfactory results. It was concluded that the in-vitro results of the Triladyl  extender yield standard values i.e. cooled and frozen-thawed quality parameters of semen and it was suggested to be used for Artificial insemination (AI).