In vitro immunological activities of the polysaccharide fraction from Haemophilus influenzae type a endotoxin

Guenounou, Moncef; Raichvarg, D; Hatat, D.; Brossard, C; Agneray, J

doi:10.1128/iai.36.2.603-608.1982

Cited by 3 publications

(1 citation statement)

References 15 publications

(15 reference statements)

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“…It has been reported that enterobacterial LPS with shorter saccharide chains had greater binding to lymphocytes and were more mitogenic than LPS with longer saccharide chains (9,40). Further, there are reports of mitogenic activity of saccharide chains isolated from Bordetella pertussis (8), H. influenzae type a (11), and Bacteroides fragilis (41). Such activity has not been described for saccharide chains from neisserial LPS, although it is reported that the binding of meningococcal LPS to human monocytes is mediated by the oligosaccharide region (12) and that the isolated chain is able to induce interleukin-1 secretion (2).…”

Section: Discussionmentioning

confidence: 99%

Enzymatically deacylated Neisseria lipopolysaccharide (LPS) inhibits murine splenocyte mitogenesis induced by LPS

1991

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Acyloxyacyl hydrolase is a leukocyte enzyme that selectively removes the secondary acyl chains from the lipid A moiety of gram-negative bacterial lipopolysaccharides (LPS). As predicted by the reported contribution of secondary acyl chains to the bioactivities of lipid A analogs, enzymatic deacylation of Salmonella typhimurium Rc LPS substantially reduces its potency in the dermal Shwartzman reaction and in several in vitro assays that measure responses of human endothelial cells and neutrophils, whereas the potency of this LPS for inducing murine splenocyte mitogenesis is affected much less. In the experiments described here, we studied the impact of acyloxyacyl hydrolysis on the bioactivities of several LPS that differ from Salmonella LPS in carbohydrate and lipid A structures. Deacylated LPS from Escherichia coli, Haemophilus influenzae, Neisseria meningitidis, and S. typhimurium were similarly reduced in potency in the Limulus lysate test (30to 60-fold reduction in potency relative to the corresponding mock-treated LPS), and the ability of all of these deacylated LPS to stimulate neutrophil adherence to human endothelial cells was reduced by a factor of 100 or more. For LPS from E. coli, H. influenzae, and Pseudomonas aeruginosa, the impact of deacylation on spleen cell mitogenesis was also similar to that observed for S. typhimurium LPS: deacylation reduced potency by less than 15-fold. Unexpectedly, the potency of Neisseria LPS in the murine splenocyte mitogenicity test was reduced over 100-fold by deacylation, and deacylated Neisseria LPS could block the mitogenic activity of Neisseria and Salmonella LPS. These studies indicate that the contribution of secondary acyl chains to the bioactivities of a given LPS cannot be predicted with confidence from the reported structure-activity relationships of lipid A or from the behavior of other deacylated LPS.

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Section: Discussionmentioning

confidence: 99%

Enzymatically deacylated Neisseria lipopolysaccharide (LPS) inhibits murine splenocyte mitogenesis induced by LPS

1991

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Action of a polysaccharide fraction ofHaemophilus influenzae lipopolysaccharide on macrophage: Implication of receptor for mannosyl-polysaccharides

Raichvarg

Hatat

Sarfati

et al. 1982

Med Microbiol Immunol

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A polysaccharide fraction (PS) was separated by mild hydrolysis from Haemophilus influenzae lipopolysaccharide. This preparation contained glycosyl-galactosyl, rhamnosyl, glucosaminyl and mannosyl residues (molar ratio: 4-1-1-2-2). It was nontoxic and immunogenic and consisted of at least one stable molecular group (fraction A; MW approximately equal to 10(6)) and an association of aggregated units (fraction B;MW approximately equal to 10(4)). This study evaluated the capacity of phagocytosis and quantitative nitroblue-tetrazolium reduction of mouse macrophages in presence of these polysaccharide fractions. After a 24-h incubation period, PS and fraction A, at 1 mg/ml, increased both phagocytosis and reduction potential of mouse peritoneal macrophages by 100%. In contrast, 1-h incubation with PS or fraction A induced a decrease of 50% in phagocytosis but no modification of NBT reduction. An identical incubation with various sugars showed that only mannosyl polymers could significantly decrease this phagocytic process. As in the case of toxic lipopolysaccharides, macrophages responded to a nontoxic preparation obtained from an endotoxin. We confirmed the role of mannosyl residues in recognition of macrophage binding receptors. Moreover, we suggest that this mannose binding ability was dependent on dose, aggregation state and molecular weight of the preparation.U

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Macrophage-dependent polyclonal activation of splenocytes by Bordetella pertussis endotoxin and its isolated polysaccharide and Lipid A regions

Haeffner‐Cavaillon

Cavaillon

Szabó

1982

Cellular Immunology

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In vitro immunological activities of the polysaccharide fraction from Haemophilus influenzae type a endotoxin

Cited by 3 publications

References 15 publications

Enzymatically deacylated Neisseria lipopolysaccharide (LPS) inhibits murine splenocyte mitogenesis induced by LPS

Enzymatically deacylated Neisseria lipopolysaccharide (LPS) inhibits murine splenocyte mitogenesis induced by LPS

Action of a polysaccharide fraction ofHaemophilus influenzae lipopolysaccharide on macrophage: Implication of receptor for mannosyl-polysaccharides

Macrophage-dependent polyclonal activation of splenocytes by Bordetella pertussis endotoxin and its isolated polysaccharide and Lipid A regions

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