In Vitro Imaging of Animal Tissue with Upconversion Nanoparticles (UCNPs) as a Molecular Probing Agent Using Swept Source Optical Coherence Tomography (SSOCT)

Mohan, M. Krishna; Maurya, S. K.; Kumar, Kaushal; Poddar, Raju

doi:10.1007/s40846-020-00511-0

Cited by 11 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optical scattering coefficient for the given set of OCT data was calculated by Beer−Lamberts law. 34 The principle followed to measure the optical scattering coefficient has also been applied to estimate the contrast to noise ratio (CNR) of tissue before and after application of UCPs. The backscattered light intensity from the different layers of the tissue decrease exponentially according to the following equation…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis of NaGdF₄:Er³⁺/Yb³⁺ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging

et al. 2020

Self Cite

View full text Add to dashboard Cite

Swept-source optical coherence tomography (SSOCT) is a noncontact, noninvasive bioimaging tool that uses various probes to obtain high contrast images. Rare-earth based upconversion particles (UCPs) provide a unique platform to enhance the capabilities of bioimaging techniques. The UCPs can act as contrast enhancers for the SSOCT with additional optical functionality, and hence in this work upconversion emission optimized polyvinylpyrrolidone (PVP)/NaGdF4:Er3+/Yb3+ UCPs were synthesized and then injected into chicken breast tissues to enhance the SSOCT images. As-synthesized samples have shown good biocompatibility toward HEK293, HeLa cell lines and showed apparent enhancement in contrast to SSOCT images. Additionally, compared to the bare NaGdF4:Er3+/Yb3+ UCPs the polyvinylpyrrolidone-stabilized UCPs have shown 3-fold enhancement in the frequency of upconverted green and red light intensity under 976 nm diode laser excitation (26 W/cm2).

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Detailed working of the constructed SSOCT can be found in our earlier report. 34 Principle of Optical Coherence Tomography (OCT). Basic optical coherence tomography (OCT) can be understood as follows.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Synthesis of NaGdF₄:Er³⁺/Yb³⁺ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…UCNPs can be employed to improve image contrast and sensitivity of in vivo imaging [ 70 , 71 ]. However, more research and testing are needed to fully comprehend the effect of nanoparticle size on optical properties to help optimize them for in vitro luminescence imaging [ 72 , 73 , 74 ]. Various imaging modalities used in pre-clinical studies and imaging science are depicted in Figure 6 .…”

Section: Applications Of Ucnpsmentioning

confidence: 99%

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

et al. 2022

View full text Add to dashboard Cite

Upconverting luminescent nanoparticles (UCNPs) are “new generation fluorophores” with an evolving landscape of applications in diverse industries, especially life sciences and healthcare. The anti-Stokes emission accompanied by long luminescence lifetimes, multiple absorptions, emission bands, and good photostability, enables background-free and multiplexed detection in deep tissues for enhanced imaging contrast. Their properties such as high color purity, high resistance to photobleaching, less photodamage to biological samples, attractive physical and chemical stability, and low toxicity are affected by the chemical composition; nanoparticle crystal structure, size, shape and the route; reagents; and procedure used in their synthesis. A wide range of hosts and lanthanide ion (Ln3+) types have been used to control the luminescent properties of nanosystems. By modification of these properties, the performance of UCNPs can be designed for anticipated end-use applications such as photodynamic therapy (PDT), high-resolution displays, bioimaging, biosensors, and drug delivery. The application landscape of inorganic nanomaterials in biological environments can be expanded by bridging the gap between nanoparticles and biomolecules via surface modifications and appropriate functionalization. This review highlights the synthesis, surface modification, and biomedical applications of UCNPs, such as bioimaging and drug delivery, and presents the scope and future perspective on Ln-doped UCNPs in biomedical applications.

show abstract

“…From then on, nanoparticles such as gold nanorods, gold nanocages, and TiO 2 nanoparticles have been reported for molecular contrast OCT [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. For example, Chen et al, presented the first scattering-dominant gold nanoparticles, nanocages, for molecular contrast OCT imaging [ 37 ].…”

Section: Conventional Octmentioning

confidence: 99%

Molecular Contrast Optical Coherence Tomography and Its Applications in Medicine

Wang

Gao

et al. 2022

IJMS

View full text Add to dashboard Cite

The growing need to understand the molecular mechanisms of diseases has prompted the revolution in molecular imaging techniques along with nanomedicine development. Conventional optical coherence tomography (OCT) is a low-cost in vivo imaging modality that provides unique high spatial and temporal resolution anatomic images but little molecular information. However, given the widespread adoption of OCT in research and clinical practice, its robust molecular imaging extensions are strongly desired to combine with anatomical images. A range of relevant approaches has been reported already. In this article, we review the recent advances of molecular contrast OCT imaging techniques, the corresponding contrast agents, especially the nanoparticle-based ones, and their applications. We also summarize the properties, design criteria, merit, and demerit of those contrast agents. In the end, the prospects and challenges for further research and development in this field are outlined.

show abstract

In Vitro Imaging of Animal Tissue with Upconversion Nanoparticles (UCNPs) as a Molecular Probing Agent Using Swept Source Optical Coherence Tomography (SSOCT)

Cited by 11 publications

References 27 publications

Synthesis of NaGdF₄:Er³⁺/Yb³⁺ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging

Synthesis of NaGdF₄:Er³⁺/Yb³⁺ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

Molecular Contrast Optical Coherence Tomography and Its Applications in Medicine

Contact Info

Product

Resources

About

In Vitro Imaging of Animal Tissue with Upconversion Nanoparticles (UCNPs) as a Molecular Probing Agent Using Swept Source Optical Coherence Tomography (SSOCT)

Cited by 11 publications

References 27 publications

Synthesis of NaGdF4:Er3+/Yb3+ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging

Synthesis of NaGdF4:Er3+/Yb3+ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging

Lanthanide-Doped Upconversion Luminescent Nanoparticles—Evolving Role in Bioimaging, Biosensing, and Drug Delivery

Molecular Contrast Optical Coherence Tomography and Its Applications in Medicine

Contact Info

Product

Resources

About

Synthesis of NaGdF₄:Er³⁺/Yb³⁺ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging

Synthesis of NaGdF₄:Er³⁺/Yb³⁺ Upconversion Particles as Exogenous Contrast Agent for Swept-Source Optical Coherence Tomography: In Vitro Animal Tissue Imaging