In vitro human cord blood platelet lysate characterisation with potential application in wound healing

Abstract

Platelets contain abundant growth factors and cytokines that have a positive influence on the migration and proliferation of different cell types by modulating its physiopathological processes. As it is known that human umbilical cord blood platelet lysate (UCB-PL) contains a supraphysiological concentration of growth factors, in the present study, we investigated its effectiveness in wound-healing processes. Human UCB-PL was obtained by the freeze/thaw of platelet concentrate (1.1 × 10 9 platelets/L), and its effect was evaluated on human or mouse endothelial cells, monocytes, fibroblasts, and keratinocytes in different concentrations. Human UCB-PL was observed to have high levels of pro-angiogenic growth factor than peripheral blood platelet-rich plasma. Among the cell lines, different concentrations of human UCB-PL were necessary to influence their viability and proliferation. For L929 cells, 5% of total volume was necessary, while for human umbilical vein endothelial cell, it was 10%. Cell migration on monocytes was increased with respect to the positive control, and scratch closure on keratinocytes was increased with respect to serum-free medium with only 10% of human UCB-PL. We concluded that the human UCB-PL may be useful to produce a large amount of standard platelet concentrates sufficient for several clinical-scale expansions avoiding inter-individual variability, which can also be used as a functional tool for clinical regenerative application for wound healing. K E Y W O R D S human umbilical cord blood, in vitro characterisation, platelet lysate, wound healing

The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

“…In addition, the metabolic activity results revealed that the NF50 and NF100 membranes served to enhance cell proliferation. Several authors that have added PL directly to culture media [5,14,15,41] have observed the positive effect of PL on cell proliferation. With respect to our study, PL in various concentrations was assembled with fibrin.…”

“…In addition, polymeric nanofibrous scaffolds can be combined with bioactive substances in order to enhance skin healing and regeneration. Wound healing is a dynamic and complex cascade of events that involves several cell types and that is governed by multiple cytokines and growth factors [5]. In some chronic wound cases, the lack of availability of growth factors negatively affects the healing cascade, and several authors have suggested the therapeutic use of these factors so as to accelerate tissue healing [6].…”

“…Several types of platelet derivatives have been described, including platelet-rich plasma, platelet gel and platelet lysate (PL), with clear evidence of their therapeutic application [12]. Of the various types of platelet derivatives, the PL effect has been widely analysed in both in vitro and in vivo studies, and the results demonstrated that PL accelerates the proliferation of fibroblasts and endothelial cells [5,13]. In addition, PL was found to enhance the proliferation and migration of keratinocytes [14,15].…”

The Provocative Roles of Platelets in Liver Disease and Cancer

“…The primary serum constituents resulting from platelet-initiated coagulation, growth, and wound repair are derived from intracellular sources (43)(44)(45)(46)(47). Circulating, resting platelets contain multiple storage granules: 1) alpha-granules (a-granules) 2) dense granules (d-granules) 3) lysosomes, and 4) microparticles (48)(49)(50).…”

Functional properties of equine adipose-derived mesenchymal stromal cells cultured with equine platelet lysate

“…In addition, others demonstrated that platelets or platelet-containing preparations can be used to promote the regenerative potential of human AD-MSC ( 30 ) and several studies have reported an improved therapeutic efficacy of MSC from different sources induced by platelet rich plasma ( 31 – 33 ). After numerous promising studies have reported the preservation of the basic characteristics of human BM- and AD-MSC with hPL ( 34 – 37 ), and functional studies using hPL ( 38 , 39 ) have also been carried out in human medicine, the time has come to perform functional assays for ePL as well.…”