In Vitro Histogenesis of Human Embryonic Stem Cells into Retina Components

Lagarkova, Maria A.; Shilov, A. A.; Gubanova, N. I.; Prokhorovich, Maria A.; Sl, Kiselev

doi:10.1007/s10517-012-1566-2

“…Insignificant amounts of retinal pigment epithelial cells can be formed during spontaneous differentiation of human pluripotent stem cells [35]. If FGF2, which is necessary to maintain the pluripotent state in culture, is removed from the culture medium, human pluripotent stem cells cultured on a mouse embryonic fibroblast substrate, matrigel, polylysine, or laminin become capable of forming pigment epithelial cells [35, 42, 43]. After 10–12 weeks of spontaneous differentiation, small pigmented regions form, which are then mechanically separated from the rest of the cell mass, yielding an almost pure cell culture of pigment epithelium ( > 99% purity).…”

Section: Directed Differentiation Of Pscs Into Rpementioning

confidence: 99%

Possibilities for Using Pluripotent Stem Cells for Restoring Damaged Eye Retinal Pigment Epithelium

Kharitonov¹,

Surdina²,

Lebedeva³

et al. 2018

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The retinal pigment epithelium is a monolayer of pigmented, hexagonal cells connected by tight junctions. These cells compose part of the outer blood-retina barrier, protect the eye from excessive light, have important secretory functions, and support the function of photoreceptors, ensuring the coordination of a variety of regulatory mechanisms. It is the degeneration of the pigment epithelium that is the root cause of many retinal degenerative diseases. The search for reliable cell sources for the transplantation of retinal pigment epithelium is of extreme urgency. Pluripotent stem cells (embryonic stem or induced pluripotent) can be differentiated with high efficiency into the pigment epithelium of the retina, which opens up possibilities for cellular therapy in macular degeneration and can slow down the development of pathology and, perhaps, restore a patient's vision. Pioneering clinical trials on transplantation of retinal pigment epithelial cells differentiated from pluripotent stem cells in the United States and Japan confirmed the need for developing and optimizing such approaches to cell therapy. For effective use, pigment epithelial cells differentiated from pluripotent stem cells should have a set of functional properties characteristic of such cells in vivo. This review summarizes the current state of preclinical and clinical studies in the field of retinal pigment epithelial transplantation therapy. We also discuss different differentiation protocols based on data in the literature and our own data, and the problems holding back the widespread therapeutic application of retinal pigment epithelium differentiated from pluripotent stem cells.

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Possibilities of an experimental damaging effect on the retinal pigment epithelium

Шеремет¹,

Mikaelyan²,

Andreev

³

et al. 2021

Vestn. oftal'mol.

1

0

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Possibilities for Using Pluripotent Stem Cells for Restoring Damaged Eye Retinal Pigment Epithelium

Kharitonov

¹

,

Surdina

²

,

Lebedeva

³

et al. 2018

Acta Naturae

10

0

View full text Add to dashboard Cite

The retinal pigment epithelium is a monolayer of pigmented, hexagonal cells connected by tight junctions. These cells compose part of the outer blood-retina barrier, protect the eye from excessive light, have important secretory functions, and support the function of photoreceptors, ensuring the coordination of a variety of regulatory mechanisms. It is the degeneration of the pigment epithelium that is the root cause of many retinal degenerative diseases. The search for reliable cell sources for the transplantation of retinal pigment epithelium is of extreme urgency. Pluripotent stem cells (embryonic stem or induced pluripotent) can be differentiated with high efficiency into the pigment epithelium of the retina, which opens up possibilities for cellular therapy in macular degeneration and can slow down the development of pathology and, perhaps, restore a patient's vision. Pioneering clinical trials on transplantation of retinal pigment epithelial cells differentiated from pluripotent stem cells in the United States and Japan confirmed the need for developing and optimizing such approaches to cell therapy. For effective use, pigment epithelial cells differentiated from pluripotent stem cells should have a set of functional properties characteristic of such cells in vivo. This review summarizes the current state of preclinical and clinical studies in the field of retinal pigment epithelial transplantation therapy. We also discuss different differentiation protocols based on data in the literature and our own data, and the problems holding back the widespread therapeutic application of retinal pigment epithelium differentiated from pluripotent stem cells.

show abstract

In Vitro Histogenesis of Human Embryonic Stem Cells into Retina Components

Abstract: We developed a protocol of in vitro differentiation of human embryonic stem cells into three-dimensional structures histologically and molecularly similar to the developing retina.

Cited by 3 publications

References 11 publications

Possibilities for Using Pluripotent Stem Cells for Restoring Damaged Eye Retinal Pigment Epithelium

Possibilities for Using Pluripotent Stem Cells for Restoring Damaged Eye Retinal Pigment Epithelium

Possibilities of an experimental damaging effect on the retinal pigment epithelium

Possibilities for Using Pluripotent Stem Cells for Restoring Damaged Eye Retinal Pigment Epithelium

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