In vitro hemocompatibility and corrosion behavior of new Zr-binary alloys in whole human blood

Abstract

The aim of this study is to evaluate the accuracy of three binary alloys’ composition, and their biocompatibility. Depending on the intended use of the medical devices made from these materials, dynamic or static tests should be performed. We have chosen static tests as we thought they may be used as knee or hip replacement, and not as cardiac valves.Three binary alloys ( Zr10Nb, Zr2.5Nb and Zr12Ta) were obtained from high purity powders (>99.9%), using an induction furnace first, and an electric arc furnace for a perfect homogenization. Their final composition was verified with a XRF analyzer-INNOV-X.Hemolysis tests can determine the degree of red blood cells lysis and the release of hemoglobin. The released hemoglobin quantity was extremely small, under 2%, in all cases, and the coagulation tests showed no risk for thrombosis. The electrochemical behavior was also studied in biological fluid, human female serum, and showed a low corrosion rate.The obtained alloys do not cause hemolysis, so they are hemocompatible with all blood types.

<p>Evaluation of Cytotoxic Effect of Cholesterol End-Capped Poly(<em>N</em>-Isopropylacrylamide)s on Selected Normal and Neoplastic Cells</p>

“…41 As proposed by Totea et al, depending on the hemolysis percentage, materials can be classified into three categories: materials resulting in over 5% hemolysis are hemolytic, between 5% and 2% are slightly hemolytic, and below 2% are nonhemolytic. 42 Hemolytic activity measurements revealed that independently of the concentration used, all tested polymers show hemolytic activity around 1% ( Figure 5A). This makes them very attractive materials for the formation of drug carriers applied via intravenous injection.…”

Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility

“…The absorption of CMH is measured at 540 nm using a photometer. Depending on hemolysis, materials can be classified in three different categories: Materials resulting in over 5% hemolysis are classified hemolytic, between 5 and 2% as slightly hemolytic, and below 2% as nonhemolytic (Totea et al, 2014 ).…”

Zirconium Alloys for Orthopaedic and Dental Applications

“…Hemocompatibility refers to the compatibility to blood and is another critically important requirement for implant materials. The hemocompatibility of three Zr-X alloys, Zr-10Nb, Zr-2.5Nb and Zr-12Ta, in human blood have been assessed and found acceptable, [44] which confirms the compatibility of Zr-alloys. Zr-coated Ti alloy implants resulted in enhanced cell responses in the early stages due to improved wettability.…”