In vitro growth response of Artemisia annua seeds to different concentrations of plant growth regulators

Jamaleddine, ZO; Lyam, Paul Terwase; Fajimi, O; Giwa, A; Aina, Abj; Lawyer, EF; Okere, AU; Odofin, WT

doi:10.5897/ajb10.1289

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…This is contrary to the findings of [24] who reported that seeds of A. annua germinated after exposure to dark. Germination of seeds of A. annua, commenced 2 -3 days after sowing and 96% of the seeds responded to treatment.…”

Section: Discussioncontrasting

confidence: 57%

In Situ Germination and Early Seedling Growth of Wormwood (Artemisia annua L.)

Muhammad¹,

Usman²,

Katung³

et al. 2014

AJPS

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Results of Analysis of Variance (ANOVA) indicated no significant difference between the seasons with respect to germination, shoot and root lengths (p ≥ 0.05). However, warm water treatment at 60˚C for 2 minutes during the rainy season trial had the least days to germination. Similarly, warm water treatments in both rain and irrigation trials at 40˚C for 3 minutes and 60˚C for 3 minutes produced the best vigor. Highest germination percent (96%) was observed during the rainy season trial with 10% Sulphuric acid treatment. Using warm water is a simple and affordable treatment especially to local farmers which will give the best result in the germination and seedling production of A. annua. Early March is recommended as the ideal planting period so that seedlings are transplanted on the field at the onset of rainfall.

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Section: Discussioncontrasting

confidence: 57%

In Situ Germination and Early Seedling Growth of Wormwood (Artemisia annua L.)

Muhammad¹,

Usman²,

Katung³

et al. 2014

AJPS

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“…Single shoots formed on auxin free MS medium [10] fortified with 0.025 mg/L BA, gave the highest number of nodes. Callus formation was evident at concentrations above 0.05 mg/L BA in combination with 0.01 mg/L NAA [11]. Similarly, the stimulating effect of plant growth regulators on shoot formation was reported for other species from the genus Artemisia such as A. judicia [12]; A. scorpia [13]; A. vulgaris [14]; A. absinthium [15]; A. sieberi [16] and A. nilagirica var.…”

Section: Introductionsupporting

confidence: 53%

Efficient protocol for mass micropropagation of Artemisia annua L.

Georgieva¹,

Atanasov²,

Hristova³

et al. 2018

GSC Biol. and Pharm. Sci.

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An efficient protocol for mass micropropagation of Artemisia annua L. was developed. The shoots were in vitro cultivated on a full and half strengths MS supplemented with BAP and IBA for shoot and root induction from stem tip explants. Maximum number of shoots (19.1) with an average shoot height of 3.6 cm was achieved at full MS medium + 1.0 mg/L BAP, after four weeks of cultivation. The best plant rooting (100%), with 7.4 average roots' number per explant and average root length of 15.1 cm, was obtained at ½ MS medium + 0.1 mg/L IBA + 2.0% sucrose, after five weeks of cultivation. Under these growing conditions, an increase of total phenolic and flavonoid content of A. annua tissues was registered. The multiplied plants were successfully ex vitro adapted and 65% survival was achieved on the mixture of soil, perlite and sand (2: 1: 1 v/v/v) used for acclimatization. The amounts of phenols and flavonoids, as well as total antioxidant activity of ex vitro plants, were significantly higher compared to those in the in vitro plants, but similar to the levels measured in vivo. The effective protocol for shoot micropropagation that was developed, could enable a large scale commercial production of A. annua.

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Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

Paul

Gupta

Khanuja

2012

Nat. Prod. Bioprospect.

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An efficient protocol for maintaining the artemisinin content in tissue culture and high frequency of in vitro direct and indirect regenerations of multiple shoots of high artemisinin yielding genotypes of Artemisia annua has been developed and their comparison with field grown mother plant has been carried out. The eleven elite genotypes (containing more than 1% artemisinin) were tested on seven different modified media formulations. Modified half MS (Murashige and Skoog's) media containing 100 mg L -1 myo-inositol, 0.5 g L -1 casine hydrolysate, 5 mg L -1 biotin, 2 mL L -1 RT (Revised Tobacco) vitamin stock, 0.5 mg L -1 BAP and 0.01 mg L -1 NAA showed best regeneration while, above modified MS medium containing 0.2 mg L -1 BAP and 0.2 mg L -1 NAA showed maximum shoot multiplication with maintained artemisinin content. Based on the chemical profiles of both the systems, minor difference was observed in their artemisinin content. A large scale culture of these plants maintained the normal growth index along with the artemisinin content and could be a better alternative to maintain the high artemisinin yielding genotypes with their genetic constraint in specific media combinations and also used as base material for further genetic improvement.

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In vitro growth response of Artemisia annua seeds to different concentrations of plant growth regulators

Cited by 6 publications

References 5 publications

<i>In Situ</i> Germination and Early Seedling Growth of Wormwood (<i>Artemisia annua</i> L.)

<i>In Situ</i> Germination and Early Seedling Growth of Wormwood (<i>Artemisia annua</i> L.)

Efficient protocol for mass micropropagation of Artemisia annua L.

Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

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In vitro growth response of Artemisia annua seeds to different concentrations of plant growth regulators

Cited by 6 publications

References 5 publications

&lt;i&gt;In Situ&lt;/i&gt; Germination and Early Seedling Growth of Wormwood (&lt;i&gt;Artemisia annua&lt;/i&gt; L.)

&lt;i&gt;In Situ&lt;/i&gt; Germination and Early Seedling Growth of Wormwood (&lt;i&gt;Artemisia annua&lt;/i&gt; L.)

Efficient protocol for mass micropropagation of Artemisia annua L.

Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

Contact Info

Product

Resources

About

<i>In Situ</i> Germination and Early Seedling Growth of Wormwood (<i>Artemisia annua</i> L.)

<i>In Situ</i> Germination and Early Seedling Growth of Wormwood (<i>Artemisia annua</i> L.)