In vitro growth of four isolates of Sclerotium rolfsii Sacc in the humid tropics

Okereke,; V., Chalapathirao Rao N. B.; Wokocha,; B.R, CHOUDHARY

doi:10.5897/ajb2007.000-2279

Cited by 16 publications

(11 citation statements)

References 2 publications

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“…The effect of temperature on sclerotium formation was also examined in other previous studies [29,31] where the optimum temperature for the better sclerotial production ranged from 20°C to 30°C with a maximum at 25°C and where pathogen failed to grow and to produce sclerotia at 10 and 40°C. Sclerotia were formed since 11 days of incubation at 28°C with no variations detected in the number and size of the sclerotia between isolates [32]. However, in the current study, the highest dry weight of 100 sclerotia was recorded at 25 and 30°C for S. rolfsii isolates Sr 1 and Sr 3 and decreased at 35°C.…”

Section: Discussioncontrasting

confidence: 66%

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Ayed¹,

Jabnoun‐Khiareddine²,

Abdallah³

et al. 2018

J Plant Pathol Microbiol

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Sclerotium rolfsii is a serious soilborne phytopathogenic fungus causing serious yield loss in crops of high economic importance. In this study, the effects of temperatures and culture media were evaluated on S. rolfsii mycelial growth, sclerotial production and germination. The three isolates tested grew over a range of temperatures from 10°C to 35°C but not at 5°C and 40°C. Radial growth and dry mycelium production were highest at 30°C. Sclerotial initiation started on the 3 rd day after incubation at 30°C and 35°C, and mature sclerotia were observed after 15 and 6 days of incubation, respectively. Optimal sclerotial production and the dry weight of 100 sclerotia varied depending on isolates and temperatures and occurred at 25°C-35°C. No mature sclerotia were produced at 5°C, 10°C, 15°C and 40°C. Sclerotial germination, noted after 24 h of incubation, was 96%-100% at 25°C-35°C, declined at 15°C-20°C, and was totally inhibited at 5°C, 10°C and 40°C even after 72 h of incubation. For all S. rolfsii isolates, optimal radial growth occurred on OAT medium followed by CZA, MYEA and PDA. When grown on PDA and OAT media, S. rolfsii developed cottony colonies with abundant mycelium whereas on WA and CZA pathogen growth was thin and scanty. Colonies formed on the other media developed sparse and flat mycelia. For sclerotial formation, WA and CZA were the most suitable culture media but no dark brown sclerotia were observed on NA and YDA. The highest sclerotial formation was observed on PDA and CZA. The highest sclerotium weight was produced by Sr 3 isolate on PDA whereas for Sr 1 and Sr 2 isolates, sclerotial weights were significantly comparable on PDA, MYEA and CZA. Sclerotial germination was optimal on all tested media except NA. The significance of these findings on S. rolfsii biology is discussed.

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Section: Discussioncontrasting

confidence: 66%

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Ayed¹,

Jabnoun‐Khiareddine²,

Abdallah³

et al. 2018

J Plant Pathol Microbiol

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“…é polífago e sua distribuição é mundial, infectando mais de 1800 espécies botânicas, podendo causar tombamento de mudas ou podridão no caule ou nas raízes das plantas hospedeiras (SMML, 2015). A partir de um micélio branco e cotonoso, produz esclerócios de 0,8 a 1,8 mm, dependendo das condições de temperatura e umidade, que é a sua estrutura principal de disseminação e sobrevivência (OKEREKE;WOKOCHA, 2007). Outro importante patógeno produtor de estrutura especializada de resistência é Macrophomina phaseolina (Tassi.).…”

Section: Survival Of Resistance Structures Inunclassified

Sobrevivência de estrutura de resistência de Macrophomina phaseolina e Sclerotium rolfsii em solo tratado biologicamente

Nascimento

Silva

Cruz

et al. 2016

AGRO@MBIENTE ON-LINE

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. An evaluation of antagonism was determined from the score allotted to the percentage of growth of isolates of Trichoderma spp. in relation to the phyto-pathogenic fungi, as well as the percentage inhibition in pathogen growth in relation to the Compost Aid ® product. The survival of resistance structures in the pathogenic fungi was evaluated 40 days after application of the treatments by plating onto a semiselective culture medium and BDA. For the experiment in vitro, all the treatments with Trichoderma produced a percentage inhibition greater than 50% for both phyto-pathogenic fungi. The commercial products Tricobiol ® and Triconemate ® gave the greatest percentage inhibition for the fungus S. rolfsii (62.5%). The Compost Aid ® product gave 100% and 98.57% inhibition in the growth of the fungi M. phaseolin and S. rolfsii respectively. The treatments in the form of a mixture of Trichoderma and Tricobiol ® had a median value of 100%, while Triconemate ® resulted in a 96% inhibition in the growth of the microsclerotia of M. phaseolina recovered from the soil; however none of these treatments inhibited sclerotial germination in S. rolfsii. The product Compost Aid ® resulted in a median of 100% and 0% growth in resistance structures for the M. phaseolina and S. rolfsii fungi respectively.

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“…Pure cultures of the final isolates were maintained on PDA slants and kept in the refrigerator until required. This method described as Okereke & Wokocha (2007).The antagonistic Trichoderma sp. was isolated from Pleurotus spent mushroom compost from (Mushroom Research Unit, Genetic Engineering and Biotechnology Research Institute (GEBRI), Menoufiya University, 2-mm PDA disks overgrown by mycelium of 5 days were collected from the outer periphery of a colony and transferred centrally in new plates, which were maintained on PDA Petri plates at 20ºC ± 1 in the dark.…”

Section: Fungal Isolates and Culture Maintenancementioning

confidence: 99%

In vitro ANTAGONISTIC ACTIONS OF HUMIC SUBSTANCE ON THE GROWTH OF Sclerotium rolfsii AND THE BIOCONTROL Trichoderma spp.

2011

Journal of Productivity and Development

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The effect of humic substance, isolated from a composting substrate, was evaluated on the mycelial growth of two strains of Sclerotium rolfsii and the antagonistic Trichoderma sp. two strains of S. rolfsii treated with humic substances, one strain isolated from tomato stems and the other isolated from the roots of sugar beet. In general, any HS treatment significantly reduced the radial growth of the S. rolfsii mycelium in normal potato dextrose agar (PDA) medium enriched with humic acid compared to potato dextrose agar medium free from humic acid. However S. rolfsii which isolated from tomato stems showed resistance against the effect of humic acid after 4 and 9 days from inoculation due to the soil treatment many times with humic acid. Humic acid treatment was also able to alter the sclerotial production either by significantly reducing the number of viable germinating sclerotia or by almost increasing the number of sclerotia to double as a resistance mechanism towards the survival of S. rolfsii. Apparently, the extent of the inhibitory action was related to some chemical and functional properties of HS, such as the COOH group content and elemental composition. On the contrary the same HS treatment generally did not inhibit the growth of Trichoderma sp. This study showed no significant correlation between HS chemical properties and Trichoderma sp. However, it showed the inhibitory effect of Trichoderma sp. in presence of humic acid on two isolates of S. rolfsii with percentage of inhibitions 53.3 % and 94.4%.

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In vitro growth of four isolates of Sclerotium rolfsii Sacc in the humid tropics

Cited by 16 publications

References 2 publications

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Sobrevivência de estrutura de resistência de Macrophomina phaseolina e Sclerotium rolfsii em solo tratado biologicamente

In vitro ANTAGONISTIC ACTIONS OF HUMIC SUBSTANCE ON THE GROWTH OF Sclerotium rolfsii AND THE BIOCONTROL Trichoderma spp.

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