In vitro growth of Carica papaya L. cotyledons

Litz, R. E.; O'Hair, Stephen K.; Conover, R. A.

doi:10.1016/0304-4238(83)90076-6

Cited by 22 publications

(6 citation statements)

References 10 publications

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“…Although plant regeneration from various explants of papaya (Carica papaya L.), such as cotyledon (Litz et al 1983), root (Chen et al 1987;Mondal et al 1994), protoplast (Chen and Chen 1992), anther (Tsay and Su 1985) and ovule (Litz and Conover 1982) have been attempted, regeneration of transformed cells from these explants into transgenic plants has not been accomplished.…”

Section: Introductionmentioning

confidence: 99%

Generation of hermaphrodite transgenic papaya lines with virus resistance via transformation of somatic embryos derived from adventitious roots of in vitro shoots

Kung

Huang

et al. 2009

Transgenic Res

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Papaya production is seriously limited by Papaya ringspot virus (PRSV) worldwide and Papaya leaf-distortion mosaic virus (PLDMV) in Eastern Asia. An efficient transformation method for developing papaya lines with transgenic resistance to these viruses and commercially desirable traits, such as hermaphroditism, is crucial to shorten the breeding program for this fruit crop. In this investigation, an untranslatable chimeric construct pYP08 containing truncated PRSV coat protein (CP) and PLDMV CP genes coupled with the 3' untranslational region of PLDMV, was generated. Root segments from different portions of adventitious roots of in vitro multiple shoots of hermaphroditic plants of papaya cultivars 'Tainung No. 2', 'Sunrise', and 'Thailand' were cultured on induction medium for regeneration into somatic embryos. The highest frequency of somatic embryogenesis was from the root-tip segments of adventitious roots developed 2-4 weeks after rooting in perlite medium. After proliferation, embryogenic tissues derived from somatic embryos were wounded in liquid-phase by carborundum and transformed by Agrobacterium carrying pYP08. Similarly, another construct pBG-PLDMVstop containing untranslatable CP gene of PLDMV was also transferred to 'Sunrise' and 'Thailand', the parental cultivars of 'Tainung No. 2'. Among 107 transgenic lines regenerated from 349 root-tip segments, nine lines of Tainung No. 2 carrying YP08 were highly resistant to PRSV and PLDMV, and 9 lines (8 'Sunrise' and 1 'Thailand') carrying PLDMV CP highly resistant to PLDMV, by a mechanism of post-transcriptional gene silencing. The hermaphroditic characteristics of the transgenic lines were confirmed by PCR with sex-linked primers and phenotypes of flower and fruit. Our approach has generated transgenic resistance to both PRSV and PLDMV with commercially desirable characters and can significantly shorten the time-consuming breeding programs for the generation of elite cultivars of papaya hybrids.

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Section: Introductionmentioning

confidence: 99%

Generation of hermaphrodite transgenic papaya lines with virus resistance via transformation of somatic embryos derived from adventitious roots of in vitro shoots

Kung

Huang

et al. 2009

Transgenic Res

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“…The regeneration of plants from anther culture in V. pubescens has not previously been reported. However, in C. papaya , there are some studies that have reported the regeneration of plants through somatic embryogenesis from anther culture (Litz and Conover, , ; Tsay and Su, ; Rimberia et al., , , ; Gyanchand et al., ). Here, plant regeneration in V. pubescens through indirect organogenesis is reported.…”

Section: Discussionmentioning

confidence: 99%

“…However, in tissue culture, many of the protocols developed for a single species can be transferable to other relatives within genera and families (Magdalita et al., ). In the Caricaceae, there are only a few reports for C. papaya being used to produce haploid and double haploid plants, and all have used anther culture (Litz and Conover, , ; Tsay and Su, ; Rimberia et al., ). For this related species, the addition of naphthalene acetic acid (NAA) and N‐(2‐chloro‐4‐pyridyl)‐N′‐phenylurea (CPPU) produced 13.8% of embryo induction when the anthers were cultivated on Murashige and Skoog (MS; Murashige and Skoog, ) basal medium.…”

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confidence: 99%

Regeneration of highland papaya (Vasconcellea pubescens) from anther culture

Chong-Pérez¹,

Carrasco

Silva

et al. 2018

Appl Plant Sci

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Premise of the Study Vasconcellea pubescens is an important Caricaceae species cultivated in several countries of South America. The objective of this study was to investigate different media compositions and plant growth regulators to induce plant regeneration.MethodsAnthers were cultured in Murashige and Skoog medium with varying concentrations of naphthalene acetic acid (NAA) and 2,4‐dichlorophenoxyacetic acid (2,4‐D) plus a cytokinin (N‐(2‐chloro‐4‐pyridyl)‐N′‐phenylurea). The effect of the basal medium supplemented with auxins and cytokinins on shoot regeneration from the induced calli was also evaluated. Addition of maltose to the basal medium was also tested.ResultsThe combination of 0.54 μM NAA and 22.66 μM 2,4‐D induced the highest rate of calli formation. Regeneration via organogenesis was obtained in Murashige and Skoog and Woody Plant Medium supplemented with maltose and containing 8.88 μM 6‐benzylaminopurine, 5.71 μM indoleacetic acid, and 2.28 μM zeatin.DiscussionThe plant regeneration protocol reported here permits the development of haploid and double haploid plants that can be useful for propagation purposes, allow a better molecular understanding of the species, and facilitate the production of new cultivars.

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“…Callusing from leaf lamina and midrib of seedlings was observed by Litz et al (1983). Shoot tip and stem segments were easier to induce callus than other explants (Dewinnaar, 1987).…”

Section: Exotic Varietiesmentioning

confidence: 99%

Papaya /

Ram¹

2009

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Preface (vit) 1. Introduction 2. Origin and history 4 3. Botany and classification-species and their relationship 6 4. Genetic resources and their conservation 19 5. Genetics and cytogenetics 24 6. Breeding for improvement 37 7. Varieties of papaya 8. Propagation, tissue culture and biotechnology 9. Soil with special reference to pH 76 10. Climate 78 11. Production technology 81 12. Parthenocarpy and unfruitfulness in papaya 13. Ripening, harvesting and yield 14. Grading, packing and storing 15. Marketing in India, ASEAN and Hawaii 16. Diseases 17. Pests and their control 18. Post-harvest physiological disorders 19. Constraints in papaya production 20. Seed production, storage and germination 21. Use of plant growth regulators in papaya 22. Papain production 23. Natural compounds in papaya 153 24. Papaya utilization and products of papaya 25. Economics of cultivation 26. Bibliography 168 27. Terminology 181 28. Subject-index 186 0-' ' Moisture 89.6% Vitamin A 2020 iu/100 g Protein 0.5% Vitamin B2 0.04 mg/100 g Fat 0.1% Vitamin C 40 mg/100 g Carbohydrate 9.5% Nicotinic acid 0.2 mg/100 g Calcium 0.01% Riboflavin 250 mg/100 g Phosphorus 0.01% Calorific value 40/100g Iron 0.4% Source: Health Bulletin. 23 1991.

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In vitro growth of Carica papaya L. cotyledons

Cited by 22 publications

References 10 publications

Generation of hermaphrodite transgenic papaya lines with virus resistance via transformation of somatic embryos derived from adventitious roots of in vitro shoots

Generation of hermaphrodite transgenic papaya lines with virus resistance via transformation of somatic embryos derived from adventitious roots of in vitro shoots

Regeneration of highland papaya (Vasconcellea pubescens) from anther culture

Papaya /

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