In vitro growth characteristics and volatile sulfur compound production of Solobacterium moorei

Stephen, Abish S.; Naughton, Declan P.; Pizzey, Robert L.; Bradshaw, David J.; Burnett, Gary R.

doi:10.1016/j.anaerobe.2014.01.007

Cited by 12 publications

(15 citation statements)

References 20 publications

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“…moorei seemed to be a low VSCs producer, compared to other oral microorganisms, and was unable to produce CH 3 SH [41]. Here, we found a moderate positive correlation between S .…”

Section: Discussionsupporting

confidence: 47%

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Changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress

Nani¹,

Lima²,

Marcondes³

et al. 2017

PLoS ONE

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ObjectiveTo investigate the associations among salivary bacteria, oral emanations of volatile sulfur compounds, and academic-related chronic stress in healthy male subjects.Materials and methodsSeventy-eight healthy male undergraduate dental students were classified as stressed or not by evaluation of burnout, a syndrome attributed to academic-related chronic stress. This evaluation was carried out using the Maslach Burnout Inventory—Student Survey questionnaire. Oral emanations of hydrogen sulfide, methyl mercaptan, and dimethyl sulfide were measured using an Oral Chroma™ portable gas chromatograph. The amounts in saliva of total bacteria and seven bacteria associated with halitosis were quantified by qPCR. The in vitro production of H2S by S. moorei and/or F. nucleatum was also measured with the Oral Chroma™ instrument.ResultsThe stressed students group showed increased oral emanations of hydrogen sulfide and dimethyl sulfide, together with higher salivary Solobacterium moorei levels (p < 0.05, Mann Whitney test). There were moderate positive correlations between the following pairs of variables: Fusobacterium nucleatum and S. moorei; F. nucleatum and hydrogen sulfide; Tannerella forsythia and F. nucleatum; T. forsythia and S. moorei. These correlations only occurred for the stressed group (p < 0.05, Spearman correlation). The in vitro experiment demonstrated that S. moorei increased H2S production by F. nucleatum (p < 0.05, ANOVA and Tukey’s test).ConclusionThe increased amount of S. moorei in saliva, and its coexistence with F. nucleatum and T. forsythia, seemed to be responsible for increased oral hydrogen sulfide in the healthy male stressed subjects.

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“…moorei seemed to be a low VSCs producer, compared to other oral microorganisms, and was unable to produce CH 3 SH [41]. Here, we found a moderate positive correlation between S .…”

Section: Discussionsupporting

confidence: 47%

“…These results are in accordance with the literature, since F . nucleatum has a moderate ability to produce H 2 S, but is one of the leading producers of CH 3 SH [7, 41]. Therefore, our findings indicate that F .…”

Section: Discussionmentioning

confidence: 73%

Changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress

Nani¹,

Lima²,

Marcondes³

et al. 2017

PLoS ONE

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“…Streptococcus salivarius K12 suppressed the growth of Solobacterium moorei , which produces H 2 S and is involved in halitosis [15, 16]. Lactobacillus salivarius WB21 and L. reuteri have been reported to inhibit oral malodor in clinical trials [17–19].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect ofEnterococcus faeciumWB2000 on Volatile Sulfur Compound Production byPorphyromonas gingivalis

Suzuki

Higuchi

Nakajima

et al. 2016

International Journal of Dentistry

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Volatile sulfur compounds (VSCs) produced by oral anaerobes are the major compounds responsible for oral malodor. Enterococcus faecium WB2000 is recognized as an antiplaque probiotic bacterium. In this study, the effect of E. faecium WB2000 on VSC production by Porphyromonas gingivalis was evaluated, and the mechanism of inhibition of oral malodor was investigated. P. gingivalis ATCC 33277 was cultured in the presence of four lactic acid bacteria, including E. faecium WB2000. Subsequently, P. gingivalis ATCC 33277, W50, W83, and two clinical isolates were cultured in the presence or absence of E. faecium WB2000, and the emission of VSCs from spent culture medium was measured by gas chromatography. The number of P. gingivalis ATCC 33277 in mixed culture with E. faecium WB2000 decreased at 6 h, and the rate of decrease was higher than that in mixed cultures with the other lactic acid bacteria. The numbers of five P. gingivalis strains decreased at similar rates in mixed culture with E. faecium WB2000. The concentration of methyl mercaptan was lower in spent culture medium from P. gingivalis and E. faecium WB2000 cultures compared with that from P. gingivalis alone. Therefore, E. faecium WB2000 may reduce oral malodor by inhibiting the growth of P. gingivalis and neutralizing methyl mercaptan.

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“…Although Gram‐negative bacteria have been recognized as a major contributor to oral malodor, many studies have shown that Gram‐positive microorganisms also play an important role (Haraszthy et al, ; Stephen, Naughton, Pizzey, Bradshaw, & Burnett, ; Vancauwenberghe et al, ). Although Gram‐positive bacteria are weak producers of VSCs, they are responsible for the deglycosylation step, which is required for the subsequent degradation of some proteins by Gram‐negative bacteria (Sterer & Rosenberg, ).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of chlorine dioxide mouthwash in reducing oral malodor: A 2‐week randomized, double‐blind, crossover study

Pham

Nguyen

2018

Clinical & Exp Dental Res

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The aim of this clinical trial was to assess the inhibitory effects of a mouthwash containing 0.1% ClO2 used for 2 weeks on oral malodor, periodontal and salivary parameters, tongue coating, and Gram‐negative and Gram‐positive bacteria in saliva. Thirty‐nine subjects with oral malodor were randomly assigned into two groups. In the first stage, one group was instructed to rinse with the experimental mouthwash (containing 0.1% ClO2), whereas the other group was instructed to rinse with the control mouthwash (sodium chloride 0.9%) for 2 weeks. After 4 weeks of washing out, in the second stage, each group then used the other mouthwash for 2 weeks. Oral malodor, periodontal status, tongue coating, salivary pH and flow rate, and the amounts of the salivary bacteria Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Solobacterium moorei, Streptococcus salivarius, Treponema denticola, and Tannerella forsythia were evaluated at baseline and after 2 weeks of mouthwash use. After 12 hr and after 2 weeks, organoleptic scores and the levels of H2S and CH3SH were significantly lower in the experimental group compared with those in the control group. After 2 weeks, the experimental mouthwash appeared significantly effective in reducing plaque index, tongue‐coating score, and the amounts of F. nucleatum, S. moorei, T. denticola, and T. forsythia in the whole saliva, compared with those at baseline. Mouthwash containing 0.1% ClO2 is effective in reducing oral malodor, dental plaque, tongue‐coating accumulation, and the amounts of F. nucleatum, S. moorei, T. denticola, and T. forsythia in saliva.

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In vitro growth characteristics and volatile sulfur compound production of Solobacterium moorei

Cited by 12 publications

References 20 publications

Changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress

Changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress

Inhibitory Effect ofEnterococcus faeciumWB2000 on Volatile Sulfur Compound Production byPorphyromonas gingivalis

Efficacy of chlorine dioxide mouthwash in reducing oral malodor: A 2‐week randomized, double‐blind, crossover study

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