In vitro growth and single-leaf photosynthetic response of Cymbidium plantlets to super-elevated CO2 under cold cathode fluorescent lamps

Norikane, Atsushi; Takamura, Tsutomu; Morokuma, Masahiro; Tanaka, Michio

doi:10.1007/s00299-010-0820-1

Cited by 24 publications

(24 citation statements)

References 45 publications

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“…''Merry'' (spath), Eucalyptus citriodora, Cymbidium spp. (boat orchid), and Oncidium hybrid (Oncidesa) (Tanaka et al 1992;Nagae et al 1996;Norikane et al 2010Norikane et al , 2013. However, as far as we know, rockwool cubes have not been used as supporting material in TIS.…”

Section: Resultsmentioning

confidence: 97%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced shoot quality and proliferation rates, and basal explants with callus produced more and longer shoots than apical and nodal segments. Use of rockwool cubes as support material prevented hyperhydricity and allowed proliferation of explants in Murashige and Skoog medium with half-strength nitrates supplemented with 0.22 lM BA and 3 % sucrose, cultured both in plantform TM and RITA Ò vessels with three or six immersions per day and additional aeration of 1 min per hour in the case of plantform TM bioreactors. Basal explants cultured in plantform TM for 5 weeks produced long shoots suitable for rooting, whereas apical and nodal explants cultured in plantform TM or RITA Ò produced shorter shoots that were suitable for maintenance of stock. For most of the clones, similar or higher proliferation rates were observed when cultured in liquid medium than in semisolid medium, with the additional benefit of cost-reduction of the former system. Shoots developed in liquid medium were submitted to ex vitro root induction by dipping in indole-3-butyric acid, and acclimatized under greenhouse conditions. This is the first demonstration of the production of chestnut plantlets from shoots cultured in liquid medium, and the protocol presented here shows good potential for application in large-scale propagation.

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Section: Resultsmentioning

confidence: 97%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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“…Kets et al, 2010; Mateos-Naranjo et al, 2010; Moutinho-Pereira et al, 2009; Norikane et al, 2010). In the marine environment, elevated P CO 2 stimulates photosynthesis and/or growth rates in macroalgae (Gao et al, 1993; Kübler et al, 1999; Xu et al, 2010) and seagrass (Palacios and Zimmerman, 2007; Jiang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Prolonged exposure to elevated CO2 promotes growth of the algal symbiont Symbiodinium muscatinei in the intertidal sea anemone Anthopleura elegantissima

Towanda

Thuesen

2012

Biology Open

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SummarySome photosynthetic organisms benefit from elevated levels of carbon dioxide, but studies on the effects of elevated PCO2 on the algal symbionts of animals are very few. This study investigated the impact of hypercapnia on a photosynthetic symbiosis between the anemone Anthopleura elegantissima and its zooxanthella Symbiodinium muscatinei. Anemones were maintained in the laboratory for 1 week at 37 Pa PCO2 and pH 8.1. Clonal pairs were then divided into two groups and maintained for 6 weeks under conditions naturally experienced in their intertidal environment, 45 Pa PCO2, pH 8.1 and 231 Pa PCO2, pH 7.3. Respiration and photosynthesis were measured after the 1-week acclimation period and after 6 weeks in experimental conditions. Density of zooxanthellal cells, zooxanthellal cell size, mitotic index and chlorophyll content were compared between non-clonemate anemones after the 1-week acclimation period and clonal anemones at the end of the experiment. Anemones thrived in hypercapnia. After 6 weeks, A. elegantissima exhibited higher rates of photosynthesis at 45 Pa (4.2 µmol O2 g−1 h−1) and 231 Pa (3.30 µmol O2 g−1 h−1) than at the initial 37 Pa (1.53 µmol O2 g−1 h−1). Likewise, anemones at 231 Pa received more of their respiratory carbon from zooxanthellae (CZAR = 78.2%) than those at 37 Pa (CZAR = 66.6%) but less than anemones at 45 Pa (CZAR = 137.3%). The mitotic index of zooxanthellae was significantly greater in the hypercapnic anemones than in anemones at lower PCO2. Excess zooxanthellae were expelled by their hosts, and cell densities, cell diameters and chlorophyll contents were not significantly different between the groups. The response of A. elegantissima to hypercapnic acidification reveals the potential adaptation of an intertidal, photosynthetic symbiosis for high PCO2.

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“…An increase in light intensity to 100 µmol m -2 s -1 PPFD has a beneficial effect on the development of structural changes in leaves associated with photoautotrophy in Gardenia jasminoides in in vitro culture [28]. In addition, the effectiveness of elevated PPFD on the in vitro growth of Cimbidium plantlets has been demonstrated; in particular the root growth was enhanced remarkably [29].…”

Section: Discussionmentioning

confidence: 94%

Innovative protocol for “ex vitro rooting” on olive micropropagation

Leva

2011

Open Life Sciences

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The commercial micropropagation of olive trees is currently limited by the production cost. An ex vitro method for olive microshoot rooting could reduce both the production cost per plant and the propagation time. In this study a successful ex vitro rooting protocol tested on seven olive cultivars is reported. The explants of cv. Maurino were collected from fifth, sixth, and seventh proliferative subcultures carried out on MSM medium, while for the other cultivars the explants were collected from only seventh proliferative subculture. Continuous light during the rooting phase was a prerequisite for the success of the ex vitro protocol. The best source of microshoots for a high rooting percentage was the seventh proliferative subculture. Cvs. Coratina, Maremmano, Maurino, Picholine, and S. Francesco showed high rooting percentages with a range of 62–76%; whereas for cvs. Correggiolo and Frantoio the experimental conditions need to be optimised. Up to 90% of the rooted microplants survived, and continuous growth of shoots was subsequently observed. The proposed protocol can be easily applied to several different olive cultivars to produce microplants by commercial laboratories. The approach makes olive micropropagation in the nursery industry both possible and profitable.

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In vitro growth and single-leaf photosynthetic response of Cymbidium plantlets to super-elevated CO2 under cold cathode fluorescent lamps

Cited by 24 publications

References 45 publications

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Prolonged exposure to elevated CO2 promotes growth of the algal symbiont Symbiodinium muscatinei in the intertidal sea anemone Anthopleura elegantissima

Innovative protocol for “ex vitro rooting” on olive micropropagation

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