In Vitro Growth and Maturation of Human B-Cell Precursors

CD10 is a marker for cycling cells with propensity to apoptosis in childhood ALL

“…For example, B cells in which the c-myc oncogene has been transfected and hyper-expressed would die in low serum concentrations in vitro unless exposed to CD40L or to an agonistic CD40 mAb which are both capable of inducing the upregulation of bcl2 and possibly of other anti-apoptotic genes (Cutrona et al, 1995). These observations suggest that a number of signals, delivered either by contact with stromal cells or by cytokines, prevent the apoptosis of CD10-positive ALL cells in vivo and facilitate their proliferation (Welch et al, 1990;Manabe et al, 1992Manabe et al, , 1994Saeland et al, 1992;Campana et al, 1994;Murti et al, 1996;Nishigaki et al, 1997). In this regard, it is of note that previous studies demonstrated that the ability of ALL cells to survive when cultured on stromal cells was an accurate predictor of a negative clinical outcome irrespective of the cell proliferative ability in vitro, possibly underlying the value of the anti-apoptotic properties of the stromal cells in the survival/expansion of the malignant ALL cells (Campana et al, 1993;Coustan-Smith et al, 1996;Kumagai et al, 1996).…”

Culture system for extensive production of CD19+IgM+ cells by human cord blood CD34+ progenitors

“…20 In addition, we also utilized SCF and G-CSF, since they synergistically support the early proliferation of murine lymphohematopoietic progenitors that can differentiate along both myeloid and Blymphoid lineages, 7 and since SCF enhances the proliferation of committed B cell progenitors in both murine and human systems. 25,26 We first tested two conditions; one was the cytokines SCF and G-CSF plus an MS-5 monolayer, and the other was SCF and G-CSF alone. Five hundred enriched CD34 + cells were plated in six-well plates in the presence of SCF and G-CSF with or without an MS-5 monolayer.…”

“…Although the stimulatory effects of SCF on murine committed B-lymphoid progenitors are evident, 25 these effects in the human system are controversial. Saeland's group 26 suggested positive roles of SCF, whereas Ryan et al 42 reported that anti-SCF Ab had no inhibitory effect on the in vitro TdT + colony formation from CD34 + CD10 − human BM cells. In addition, Namikawa et al 43 observed stimulatory roles of FLT3/FLK2 ligand, but not of SCF on the proliferation of CD34 + CD19 + pro-B cells.…”

CD40 ligand upregulates expression of the IL-3 receptor and stimulates proliferation of B-lineage acute lymphoblastic leukemia cells in the presence of IL-3

“…[13][14][15] In these studies, IL-3 acted as a specific co-signal with anti-CD40 antibody for stimulating proliferation of normal BCP-cells, and proliferation was further enhanced by addition of IL-7 and IL-10. In the present study, addition of IL-7 to cultures with CD40L + IL-3 increased the proliferation and growth rate of BCP-ALL cells from some cases.…”

“…Wolf et al 12 reported that proliferation of normal human BCP cells in bone marrow stroma-supported cultures was enhanced by addition of exogenous IL-7. Saeland et al 13 reported that IL-3 stimulated proliferation of normal BCP-cells in cultures with or without stromal cell support. Furthermore, the stimulatory effect of both IL-3 and IL-7 on normal BCP-cells was remarkably increased following activation of CD40 by anti-CD40 antibody.…”