In vitro glucose uptake by isolated rat hemi-diaphragm study of Aegle marmelos Correa root

Ravi, Subban; Sadashiva, C. T.; Tamizmani, T.; Balasubramanian, T.; Rupeshkumar, Mani; Balachandran, Indira

doi:10.3329/bjp.v4i1.1211

Cited by 7 publications

(4 citation statements)

References 12 publications

(14 reference statements)

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“…The possible reason for the observed attenuation of glucose uptake (transport) promoting activity of the alcoholic extracts compared to insulin could be the presence of other interfering substances in the crude extracts that reduce the potential of the promoting molecules. Presence of phytochemicals in alcoholic extracts of other plants, which promote glucose uptake in muscle and adipose cell lines [ 21 - 23 ], substantiate our observed effect.…”

Section: Discussionsupporting

confidence: 82%

Antioxidant rich flavonoids from Oreocnide integrifolia enhance glucose uptake and insulin secretion and protects pancreatic β-cells from streptozotocin insult

Ansarullah

Bharucha

Dwivedi

et al. 2011

BMC Complement Altern Med

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BackgroundInsulin deficiency is the prime basis of all diabetic manifestations and agents that can bring about insulin secretion would be of pivotal significance for cure of diabetes. To test this hypothesis, we carried out bioactivity guided fractionation of Oreocnide integrifolia (Urticaceae); a folklore plant consumed for ameliorating diabetic symptoms using experimental models.MethodsWe carried out bioassay guided fractionation using RINmF and C2C12 cell line for glucose stimulated insulin secretion (GSIS) and glucose uptake potential of fractions. Further, the bioactive fraction was challenged for its GSIS in cultured mouse islets with basal (4.5 mM) and stimulated (16.7 mM) levels of glucose concentrations. The Flavonoid rich fraction (FRF) was exposed to 2 mM streptozotocin stress and the anti-ROS/RNS potential was evaluated. Additionally, the bioactive fraction was assessed for its antidiabetic and anti-apoptotic property in-vivo using multidose streptozotocin induced diabetes in BALB/c mice.ResultsThe results suggested FRF to be the most active fraction as assessed by GSIS in RINm5F cells and its ability for glucose uptake in C2C12 cells. FRF displayed significant potential in terms of increasing intracellular calcium and cAMP levels even in presence of a phosphodiesterase inhibitor, IBMX in cultured pancreatic islets. FRF depicted a dose-dependent reversal of all the cytotoxic manifestations except peroxynitrite and NO formation when subjected in-vitro along with STZ. Further scrutinization of FRF for its in-vivo antidiabetic property demonstrated improved glycemic indices and decreased pancreatic β-cell apoptosis.ConclusionsOverall, the flavonoid mixture has shown to have significant insulin secretogogue, insulinomimetic and cytoprotective effects and can be evaluated for clinical trials as a therapeutant in the management of diabetic manifestations.

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Section: Discussionsupporting

confidence: 82%

Antioxidant rich flavonoids from Oreocnide integrifolia enhance glucose uptake and insulin secretion and protects pancreatic β-cells from streptozotocin insult

Ansarullah

Bharucha

Dwivedi

et al. 2011

BMC Complement Altern Med

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“…Similarly, Panda and Kar () reported that scopoletin has potent antioxidative activities and it is able to regulate hyperglycemia and diabetic complications in rats. Both skimmianine and melicarpinone are quinolone alkaloids that have anti‐inflammatory activity and maybe responsible for the in vitro antidiabetic effects (Ravi and others ).…”

Section: Resultsmentioning

confidence: 99%

Potent Antidiabetic Activity and Metabolite Profiling of Melicope Lunu‐ankenda Leaves

AL-Zuaidy

Hamid

Ismail

et al. 2016

Journal of Food Science

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Diabetes mellitus is normally characterized by chronic hyperglycemia associated with disturbances in the fat, carbohydrate, and protein metabolism. There is an increasing trend of using natural products instead of synthetic agents as alternative therapy for disorders due to their fewer side effects. In this study, antidiabetic and antioxidant activities of different Melicope lunu-ankenda (ML) ethanolic extracts were evaluated using inhibition of α-glucosidase and 2,2-diphenyl-l-picrylhydrazyl (DPPH) radicals scavenging activity, respectively; whereas, proton nuclear magnetic resonance ((1) H NMR) and ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS) techniques were used for metabolite profiling of ML leaf extracts at different concentrations of ethanol and water. Sixty percent of ethanolic ML extract showed highest inhibitory effect against α-glucosidase enzyme (IC50 of 37 μg/mL) and DPPH scavenging activity (IC50 of 48 μg/mL). Antidiabetic effect of ML extracts was also evaluated in vivo and it was found that the high doses (400 mg/Kg BW) of ML extract exhibited high suppression in fasting blood glucose level by 62.75%. The metabolites responsible for variation among ML samples with variable ethanolic levels have been evaluated successfully using (1) H-NMR-based metabolomics. The principal component analysis (PCA) and partial least squares(PLS) analysis scores depicted clear and distinct separations into 4 clusters representing the 4 ethanolic concentrations by PC1 and PC2, with an eigenvalue of 69.9%. Various (1) H-NMR chemical shifts related to the metabolites responsible for sample difference were also ascribed. The main bioactive compounds identified attributing toward the separation included: isorhamnetin, skimmianine, scopoletin, and melicarpinone. Hence, ML may be used as promising medicinal plant for the development of new functional foods, new generation antidiabetic drugs, as a single entity phytomedicine or in combinational therapy.

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“…Ethnopharmacological and ethno botanical surveys indicate that more than 1200 plants are used worldwide in herbal medicine to treat diabetes (Ziyyat et al, 1997;El-Hilaly et al, 2003;Tahraoui et al, 2007 (Prajapati et al, 2008;Ravi et al, 2009;Pattanayak et al, 2009;Tatiya et al, 2013;Bandara et al, 2009;Saha et al, 2009;Bhowmik et al, 2009;Sen et al, 2013;Khanam et al, 2009;Alamgeer et al, 2013;Bhavsar and Talele, 2013;Kulandaivel et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Antihyperlipidemic and renoprotective activities of methanolic extract of <i>Canscora decussata</i> extract in alloxan-induced diabetic rabbits

Irshad

Akhtar

Kamal

et al. 2013

Bangladesh J Pharmacol

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The current study was designed to evaluate the hypolipidemic and renoprotective effects of methanolic extract of powdered Canscora decussata whole plant in the diabetic rabbits. Thirty rabbits were divided into five groups having 6 animals each including normal and diabetic controls groups, the remaining groups received methanolic extract in 400 and 600 mg/kg doses and another group got pioglitazone (3 mg/kg) for 30 days. Serum levels of triglycerides, total cholesterol, LDL-cholesterol, HDL-cholesterol, albumin, globulin and total proteins were estimated by using commercially available kits. The results showed that extract significantly (p<0.01) decreased the raised parameters including triglyceride, total cholesterol and LDL-cholesterol, atherogenic index, Coronary risk index up to normal values compared to diabetic rabbits. However, it significantly increased HDL-cholesterol, albumin, globulin and total protein levels. Therefore, it is suggested that methanolic extract of C. decussata exerts hypolipidemic and renoprotective effects in the alloxan-induced diabetic rabbits. Article Info

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In vitro glucose uptake by isolated rat hemi-diaphragm study of Aegle marmelos Correa root

Cited by 7 publications

References 12 publications

Antioxidant rich flavonoids from Oreocnide integrifolia enhance glucose uptake and insulin secretion and protects pancreatic β-cells from streptozotocin insult

Antioxidant rich flavonoids from Oreocnide integrifolia enhance glucose uptake and insulin secretion and protects pancreatic β-cells from streptozotocin insult

Potent Antidiabetic Activity and Metabolite Profiling of Melicope Lunu‐ankenda Leaves

Antihyperlipidemic and renoprotective activities of methanolic extract of <i>Canscora decussata</i> extract in alloxan-induced diabetic rabbits

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