In vitro genotoxicity assessment and 28-day repeated dose oral toxicity study of steady-calcium formula in rats

Chang, Ting-Yu; Lan, Kuo‐Cheng; Hua, Ke; Liu, Shing‐Hwa

doi:10.1016/j.toxrep.2022.04.008

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…Prior toxicity test results have shown that CSC supplements are safe. 12 Food intake and body weight will increase as a result of estrogen deficiency. 13,14 The food intake of the rats in the OVX groups increased in the first six weeks of the test, and the body weight was obviously larger than that of the sham group in the process, meaning the post-menopausal symptoms in the experimental animals were successfully presented by the ovariectomy procedure.…”

Section: Discussionmentioning

confidence: 99%

Effect of Compound Supplement on the Bone Health of Rats with Osteoporosis Induced by Ovariectomy

Chu,

Lin,

Yang

2023

Osteol Rheumatol Open J

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Background: The World Health Organization (WHO) has recognized osteoporosis as the second most serious epidemic in the world. There are many factors in the occurrence of osteoporosis, among which amenorrhea and aging are major causes, therefore the risk of osteoporosis increases with age. Moreover, osteoporosis influences personal health and mobility, and in turn, greatly increases national healthcare expenses. It is known that chicken sternal cartilage (CSC) hydrolysate, salmon nasal cartilage extract, turmeric (Curcuma longa L.) extract, mangosteen (Garcinia mangostana) extract, soybean extract, calcium citrate, vitamin C, and vitamin D have the potential to reduce bone loss and protect bones. However, this novel combination for improving bone loss has not yet been studied or discussed. Objective: This study discussed the effect of a CSC formula containing chicken sternal cartilage hydrolysate, salmon nasal cartilage extract, turmeric extract, mangosteen extract, soybean extract, calcium citrate, vitamin C, and vitamin D on improving the osteoporosis of rats which had received ovariectomy. Their body weight, body length, serum biochemical value, bone tissue, and biomechanics were analyzed. Design: The 10-week-old female Sprague-Dawley (SD) rats used in this study were randomly divided into six groups for the experiment, including the sham group (10 rats) and five ovariectomy (OVX) groups (53 rats). Two weeks after the ovariectomy, the following doses were administered to the corresponding groups respectively every day: carboxymethyl cellulose (CMC) solvent group (represented by OVX+CMC), and CSC 339 mg/kg (represented by OVX+CSC-L), CSC 678 mg/kg (represented by OVX+CSC-M), CSC 1356 mg/kg (represented by OVX+CSC-H). The positive control (alendronate, 2.5 mg/kg represented by OVX+AD) was administered three times weekly. The rats were sacrificed 14-weeks after administration of the experimental materials. Results: According to the experimental results, in comparison to the OVX+CMC group, the OVX+CSC-L group, OVX+CSCM group, and OVX+CSC-H group could significantly reduce the N-terminal telopeptides of type I collagen, the N-telopeptide (NTX) concentration, and the osteocalcin concentration in the serum. According to the micro-computed tomography analysis, in comparison to the OVX+CMC group, the femoral bone mineral density, trabecular area percentage, and trabecular thickness of the OVX+CSC-L, OVX+CSC-M, and OVX+CSC-H groups increased significantly, and the trabecular separation decreased significantly. According to the biomechanical analysis, in comparison to the OVX+CMC group, the OVX+CSC-L, OVX+CSC-M, and OVX+CSC-H groups could increase the maximum load, hardness, absorbed energy, and Young’s modulus of the femur significantly. Conclusion: CSC can increase the bone mineral density, trabecular area percentage, and trabecular thickness significantly; it can reduce the trabecular separation, NTX and osteocalcin (OCN) concentrations in serum significantly, and it can retard the bone loss induced by ovariectomy effectively, as well as protect bones. It can be used as a mild and long-term dietary supplement; however, the result of long-term administration to the human body should be observed in the future.

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Section: Discussionmentioning

confidence: 99%

Effect of Compound Supplement on the Bone Health of Rats with Osteoporosis Induced by Ovariectomy

Chu,

Lin,

Yang

2023

Osteol Rheumatol Open J

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“…The approach described by Sannad et al 38 and Wang et al 39 was used to estimate the biochemical parameters such as total protein and total bilirubin. Chang et al 40 and Zhang et al 41 techniques were used to assess the SGOT, SGPT and serum ALP. The technique of Musso et al 42 was used to determine the levels of urea, uric acid, and creatinine in serum.…”

Section: Biochemical and Hematological Parametersmentioning

confidence: 99%

Syringodium isoetifolium Fosters an Antioxidant Defense System, Modulates Glycolytic Enzymes and Protects Membrane Integrity in DEN-induced Hepatocellular Carcinoma in Albino Wistar Rats

Kavitha,

Padmini,

Dhanaraju

et al. 2023

Ind. J. Pharm. Edu. Res.

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Background: Syringodium isoetifolium seagrass has bioactive constituents with potential pharmacological uses, but their use is limited owing to scarce scientific evidence. The in vivo anti-cancer activity of Syringodium isoetifolium against DEN-induced hepatocellular carcinoma in Wistar albino rats is described in this work for the first time. Materials and methods: Wistar albino rats were used as test subjects to examine the anti-cancer properties of Syringodium isoetifolium against DEN-induced hepatocellular carcinoma at the dose of 50 mg/kg body weight. The experimental rats were split into five groups (Group I-V). Except for group I, remaining all animals received DEN and Phenobarbitone during the experiment. Group I and Group II acted as normal and diseased control groups respectively. The extracts were administered to the satellite group III and IV orally with the dose of 250 and 500 mg/kg body weight respectively. 5 fluorouracil 20mg/ kg was administered to group V orally and considered as a standard. The total experimental period lasted for 14 weeks. Results: The findings show that Syringodium isoetifolium significantly reduces liver tumor volume, burden and numbers in experimental rats (p<0.05) when compared to the control group. Besides, the extracts treated groups restored the pathological parameters close to normal values (p<0.05). The histological analysis also showed that the extract-treated animals' livers had recovered their normal architecture. Conclusion: The study concludes that Syringodium isoetifolium inhibits the cancer growth in hepatocellular carcinoma by altering the antioxidant defense system, glycolysis and protecting the membrane architecture by inhibiting the elevated levels of haematological, biochemical parameters and biomarker values.

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In vitro genotoxicity assessment and 28-day repeated dose oral toxicity study of steady-calcium formula in rats

Cited by 2 publications

References 34 publications

Effect of Compound Supplement on the Bone Health of Rats with Osteoporosis Induced by Ovariectomy

Effect of Compound Supplement on the Bone Health of Rats with Osteoporosis Induced by Ovariectomy

Syringodium isoetifolium Fosters an Antioxidant Defense System, Modulates Glycolytic Enzymes and Protects Membrane Integrity in DEN-induced Hepatocellular Carcinoma in Albino Wistar Rats

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