In vitro-generation of surrogate islets from adult stem cells

Peck, Ammon B.; Ramiya, Vijayakumar K.

doi:10.1016/j.trim.2003.12.011

Cited by 13 publications

(2 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The five genes that were examined in this comparison were the pancreatic endocrine hormone genes (insulin, glucagon, somatostatin, and pancreatic polypeptide) as well as the pdx-1 gene. Pdx-1 was investigated since it is expressed by early pancreatic progenitor cell populations as well as by mature beta cells (Peck and Ramiya, 2004). This comparison highlighted that there was a threefold lower insulin expression, a twofold higher glucagon expression, a sevenfold higher somatostatin expression, a 19-fold higher pancreatic polypeptide expression, and a 1.2-fold higher pdx-1 expression in the cells cultured in PPRF-psc1 medium in comparison to human islets.…”

Section: Characterization Of Expanded Cell Populations From Adherent mentioning

confidence: 99%

Characterization of human islet‐like structures generated from pancreatic precursor cells in culture

Bodnar

Sen

Kallos

et al. 2005

Biotech & Bioengineering

View full text Add to dashboard Cite

This study addresses the characterization of human islet-like structures generated from a newly discovered sparse population of precursor cells (Petropavlovskaia and Rosenberg, 2002) in the human pancreas. These cells may be progenitor cells capable of producing pancreatic cells suitable for the treatment of type 1 diabetes. The cells were cultured successfully in non-adherent stationary cultures and yielded, as an important first step, a 1.9-fold expansion in a serum-free medium developed specifically for this cell type. This expanded population grew as pancreatic cell aggregates, which were analyzed for islet-like characteristics. Specifically, through RT-PCR analyses and functionality assays, we show that cells within the population expressed all four of the endocrine hormone genes and proteins (insulin, glucagon, somatostatin, pancreatic polypeptide). As well, the expanded pancreatic precursor cell population exhibited glucose responsiveness although the produced cells appeared to be still primitive in nature.

show abstract

Section: Characterization Of Expanded Cell Populations From Adherent mentioning

confidence: 99%

Characterization of human islet‐like structures generated from pancreatic precursor cells in culture

Bodnar

Sen

Kallos

et al. 2005

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Several studies report the production of islet‐like structures in the porcine, mouse, and human models (8–16). In particular, one study performed on human ductal tissue reported a 7‐fold increase in DNA content along with a 10‐ to 15‐fold increase in insulin content over 3−4 weeks of culture in a serum‐free medium (8).…”

Section: Introductionmentioning

confidence: 99%

Production of Islet-Like Structures from Neonatal Porcine Pancreatic Tissue in Suspension Bioreactors

et al. 2006

View full text Add to dashboard Cite

The aim of this study was to develop a scaleable process to expand pancreatic endocrine tissue (i.e., aggregates or islet-like structures) in suspension bioreactors. Key issues addressed included (i) serum-free media, (ii) cell inoculation density, (iii) medium pH, and (iv) aggregate dissociation. Suspension bioreactors were inoculated with pancreatic neonatal tissue and operated under controlled conditions for a 9-day period. Medium studies showed that a new serum-free medium developed in our laboratory was capable of supporting endocrine cell expansion. An inoculation density of 127,000 cells/mL resulted in more than a 7.5-fold increase in the number of insulin-positive cells after 9 days. The resulting population consisted of single cells and many islet-like aggregates that contained all of the endocrine cell types (including insulin-positive, glucagon-positive, somatostatin-positive, and pancreatic polypeptide-positive cells). Furthermore, the cell aggregates exhibited a glucose-responsive behavior. This study represents a significant milestone on the path to the effective expansion of human islet-like tissue in bioreactors that may be used for cell therapy to treat Type 1 diabetes.

show abstract

Pancreas Engineering

Cortesini¹,

Calafiore²

Fundamentals of Tissue Engineering and Regenerative Medicine

View full text Add to dashboard Cite

In vitro-generation of surrogate islets from adult stem cells

Cited by 13 publications

References 96 publications

Characterization of human islet‐like structures generated from pancreatic precursor cells in culture

Characterization of human islet‐like structures generated from pancreatic precursor cells in culture

Production of Islet-Like Structures from Neonatal Porcine Pancreatic Tissue in Suspension Bioreactors

Pancreas Engineering

Contact Info

Product

Resources

About