In vitro generation of osteochondral differentiation of human marrow mesenchymal stem cells in novel collagen–hydroxyapatite layered scaffolds

Zhou, Jiaan; Xu, Caixia; Wu, Gang; Cao, Xiaodong; Zhang, Liangming; Zhai, Zhichen; Zheng, Zhiwen; Chen, Xiaofeng; Wang, Yingjun

doi:10.1016/j.actbio.2011.06.040

Cited by 89 publications

(75 citation statements)

References 21 publications

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“…Real-time polymerase chain reactions (PCR) were performed in triplicate using the QuantiTect SYBR Green PCR (Qiagen) kit in an Applied Biosystems 7900HT Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA). All primer sequences used were taken from the literature 30,31 (Table 1) and were synthesized by Integrated DNA Technologies (Coralville, IA). Expression levels of the chondrogenic markers collagen type II alpha 1 (COL2A1), aggrecan (ACAN), and sex determining region Y box 9 (SOX9) as well as osteogenic markers runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP), alkaline phosphatase (ALP), and osteocalcin (OCN) were quantified with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as a housekeeping gene.…”

Section: Quantification Of Scaffold Contractionmentioning

confidence: 99%

Collagen-GAG Scaffold Biophysical Properties Bias MSC Lineage Choice in the Presence of Mixed Soluble Signals

Caliari

Harley

2014

Tissue Engineering Part A

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Biomaterial strategies for regenerating multitissue structures require unique approaches. One strategy is to design scaffolds so that their local biophysical properties can enhance site-specific effects of an otherwise heterogeneous biomolecular environment. This investigation examined the role of biomaterial physical properties (relative density, mineral content) on the human mesenchymal stem cell phenotype in the presence of mixed soluble signals to drive osteogenesis or chondrogenesis. We tested a series of three-dimensional collagen-glycosaminoglycan scaffolds with properties inspired by extracellular matrix characteristics across the osteotendinous interface (tendon, cartilage, and bone). We found that selective scaffold mineralization induced a depressed chondrogenic response compared with nonmineralized groups as demonstrated by gene expression and histological analyses. Interestingly, the greatest chondrogenic response was found in a higher density, nonmineralized scaffold variant despite increased contraction and cellular condensation in lower density nonmineralized scaffolds. In fact, the lower density scaffolds demonstrated a significantly higher expression of osteogenic transcripts as well as ample mineralization after 21 days of culture. This effect may be due to local stiffening of the scaffold microenvironment as the scaffold contracts, leading to increased cell density, accelerated differentiation, and possible endochondral ossification as evidenced by a transition from a glycosaminoglycan (GAG)-rich milieu to higher mineralization at later culture times. These findings will help shape the design rules for graded biomaterials to regenerate distinct fibrillar, fibrocartilagenous, and mineralized regions of orthopedic interfaces.

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Section: Quantification Of Scaffold Contractionmentioning

confidence: 99%

Collagen-GAG Scaffold Biophysical Properties Bias MSC Lineage Choice in the Presence of Mixed Soluble Signals

Caliari

Harley

2014

Tissue Engineering Part A

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“…As a composite matrix, this material combination provided a microstructure that attempted to mimic native bone and provided a suitable microenvironment for new bone formation in vivo (Table 2). 102 Zhou et al 103 demonstrated a similar strategy by formulating bilayered osteochondral scaffolds that consisted of a collagen type I layer…”

Section: Hydroxyapatitementioning

confidence: 99%

“…The biphasic scaffolds were seeded with human mesenchymal stem cells (MSCs) and cultured separately in chondrogenic and osteogenic medium (Table 5). 103 Li et al 104 constructed a composite of poly(L-lactic) acid (PLLA), chitosan, and HAp microspheres as a hybrid bone tissue engineering composite and studied the cellular response to these constructs in vitro using murine calvarial osteoblasts (Table 1). Approaches by each of these groups demonstrated the growing tendency of raw materials to be utilized as building blocks in bone tissue engineering scaffolds.…”

mentioning

confidence: 99%

Leveraging “Raw Materials” as Building Blocks and Bioactive Signals in Regenerative Medicine

Renth

Detamore

2012

Tissue Engineering Part B: Reviews

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“…The scaffolds revealed an optimized environment for cell attachment and proliferation. In another study, Zhou et al [7] developed also a collagen-based layered scaffold composed by a collagen and a collagen/HA part to mimic the cartilage and bone regions, respectively. Human mesenchymal stem cells were used to promote chondrogenic and osteogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Current advances in solid free-form techniques for osteochondral tissue engineering

et al. 2018

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Osteochondral (OC) lesions are characterized by defects in two different zones, the cartilage region and subchondral bone region. These lesions are frequently associated with mechanical instability, as well as osteoarthritic degenerative changes in the knee. The lack of spontaneous healing and the drawbacks of the current treatments have increased the attention from the scientific community to this issue. Different tissue engineering approaches have been attempted using different polymers and different scaffolds' processing. However, the current conventional techniques do not allow the full control over scaffold fabrication, and in this type of approaches, the tuning ability is the key to success in tissue regeneration. In this sense, the researchers have placed their efforts in the development of solid free-form (SFF) techniques. These techniques allow tuning different properties at the micro-macro scale, creating scaffolds with appropriate features for OC tissue engineering. In this review, it is discussed the current SFF techniques used in OC tissue engineering and presented their promising results and current challenges.

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In vitro generation of osteochondral differentiation of human marrow mesenchymal stem cells in novel collagen–hydroxyapatite layered scaffolds

Cited by 89 publications

References 21 publications

Collagen-GAG Scaffold Biophysical Properties Bias MSC Lineage Choice in the Presence of Mixed Soluble Signals

Collagen-GAG Scaffold Biophysical Properties Bias MSC Lineage Choice in the Presence of Mixed Soluble Signals

Leveraging “Raw Materials” as Building Blocks and Bioactive Signals in Regenerative Medicine

Current advances in solid free-form techniques for osteochondral tissue engineering

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