In vitro generation of high artemisinin yielding salt tolerant somaclonal variant and development of SCAR marker in Artemisia annua L.

Pandey, Neha; Meena, Raj Kumar; Kumar, Sanjay; Pandey-Rai, Shashi

doi:10.1007/s11240-016-1050-1

Cited by 17 publications

(5 citation statements)

References 31 publications

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“…The leaf size is primarily controlled by growth environment, whereas leaf hair initiation is genetically based (Wilkens et al 1996;Roy et al 1999). When the environment influences the rates of cell division and/or cell expansion after the leaf hairs are differentiated, modifications of leaf hair density might also occur (Pandey et al 2016). This might be the explanation of what we observed in our study regarding the carrot.…”

Section: Discussionmentioning

confidence: 99%

Application of the salt stress to the protoplast cultures of the carrot (Daucus carota L.) and evaluation of the response of regenerants to soil salinity

Kiełkowska

Grzebelus

Lis-Krzyścin

et al. 2019

Plant Cell Tiss Organ Cult

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This is the first study to generate carrot plants for enhanced salinity tolerance using a single-cell in vitro system. Protoplasts of three carrot accessions were exposed to treatment by seven different concentrations of NaCl (10-400 mM). Salt concentrations higher than 50 mM decreased plating efficiency and those of 200-400 mM of NaCl completely arrested mitotic divisions of cultured cells. The protoplast-derived plants from the control and 50-100 mM NaCl treatment were subjected to an 8-week salt stress in greenhouse conditions induced by salinized soil (EC 3 and 6 mS cm −1 ). 50 mM NaCl stress applied in vitro induced polyploidy among regenerated plants. The regenerants obtained from the 50 and 100 mM NaCl-treated protoplast cultures grown in saline soil had a higher survival rate compared to the regenerants from the control cultures. The salt-stressed plants accumulated anthocyanins in petioles and produced denser hairs on leaves and petioles in comparison to the control plants. Salt stress influenced pollen viability and seed setting of obtained regenerants. The results suggest that salt stress applied in vitro in protoplast cultures creates variation which allows alleviating the negative effects of salt stress on the development and reproduction of the carrot. Key messageSalt stress applied to carrot protoplasts generates variability manifested in differences in cellularresponse and variation in ploidy. The adaptation of carrot regenerants to soil salinity was associatedwith accumulation of anthocyanins and increased hairiness.

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Section: Discussionmentioning

confidence: 99%

Application of the salt stress to the protoplast cultures of the carrot (Daucus carota L.) and evaluation of the response of regenerants to soil salinity

Kiełkowska

Grzebelus

Lis-Krzyścin

et al. 2019

Plant Cell Tiss Organ Cult

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“…It is known that the salinity resistant mutants of Catharanthus roseus produce and accumulate alkaloids in high concentrations due to hypomethylation (epigenetic change) in their genomic DNA (Kumari et al, 2013). A saline resistant somaclone of A. annua is known to express ART pathway at high levels and accumulate artemisinin in higher concentration than the control plants (Pandey et al, 2016). To develop artemisinin and essential oil rich genotypes that carry pleiotropic changes in characters that determine the expression of terpenoids, via induction of salt tolerant mutations, is a highly promising experimental area to persue.…”

Section: Salinity Resistant Mutantsmentioning

confidence: 99%

Artemisia (Asteraceae) essential oils: compositional variation and mechanisms of its origin, biosynthesis of constituents, correspondence between biological activities and ethnomedicinal usage and repurposement prospects

Kumar

Goel²,

Singh³

et al. 2019

PINSA

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Many species of the genus Artemisia, a taxon in the angiosperm family Asteraceae composed of more than 500 species, are widely used in traditional medicine, on account of the safe multi-curative properties of its secondary metabolites biosynthesized in chloroplasts and cytoplasm of cells, largely in trichomes. The steam distilled Artemisia essential oils, into which the volatile organic metabolites get extracted, have been observed to demonstrate enormous intra-and interspecies variation. This review summarises for the Artemisia species (artemisias) the nature of compositional variation of the essential oil volatiles, biosynthetic processes of the major classes of the observed volatiles, and mechanisms responsible for the variation in the content of volatiles in essential oils. The interrelationships between the biosyntheses of volatiles of essential oil and the antimalarial compound artemisinin are delineated. Further, the relationships between ethnomedicinal uses of various artemisias and biological activities detected in their essential oils are discussed with reference to the quality of essential oils. Artemisia essential oils offer highly significant repurposement prospects. Future directions of research on artemisias are also outlined.

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“…annua. − Thus, it is essential to find pathways to augment the content of artemisinin in the native plant. Research achievements in the direction of high-artemisinin plant production include the establishment of mutant libraries, construction of a tetraploid cultivar with high artemisinin content, increasing the number of glandular trichomes where artemisinin is biosynthesized and accumulated, overexpression of the transcription factor that positively regulates the artemisinin biosynthetic pathway in transgenic A. annua plants, , and so forth.…”

Section: Introductionmentioning

confidence: 99%

Side Products of Recombinant Amorpha-4,11-diene Synthase and Their Effect on Microbial Artemisinin Production

Huang

Tian

et al. 2021

J. Agric. Food Chem.

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Amorpha-4,11-diene synthase (ADS) is the first committed enzyme in the biosynthesis of artemisinin. Artemisinin production by biobased fermentation is considered a reliable alternative pathway. Heterologously expressed ADS has been established to generate several minor products, including structural analogues of amorpha-4,11-diene, but their fate in fermentation is still unknown. Here, using chiral analysis, we found that ADS produces one of the analogues, amorpha-4-en-11-ol, as a pair of epimers. Labeling experiments revealed that ADS mutants yielded amorphene-type sesquiterpenes, indicating the co-occurrence of initial 1,6 and 1,10 cyclization of farnesyl diphosphate in a single enzyme. Interestingly, the immediate downstream oxidase CYP71AV1 had very low affinity to the side products of the recombinant ADS, including amorpha-4-en-7-ol, which is structurally similar to amorpha-4,11-diene. Our data uncover the complex catalytic mechanism of recombinant ADS and reveal a potential negative effect of the side products of recombinant ADS on the production of the artemisinin precursor in microbes.

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In vitro generation of high artemisinin yielding salt tolerant somaclonal variant and development of SCAR marker in Artemisia annua L.

Cited by 17 publications

References 31 publications

Application of the salt stress to the protoplast cultures of the carrot (Daucus carota L.) and evaluation of the response of regenerants to soil salinity

Application of the salt stress to the protoplast cultures of the carrot (Daucus carota L.) and evaluation of the response of regenerants to soil salinity

Artemisia (Asteraceae) essential oils: compositional variation and mechanisms of its origin, biosynthesis of constituents, correspondence between biological activities and ethnomedicinal usage and repurposement prospects

Side Products of Recombinant Amorpha-4,11-diene Synthase and Their Effect on Microbial Artemisinin Production

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