In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field

Girolamo, Laura de; Viganò, Marco; Galliera, Emanuela; Stanco, D.; Setti, Stefania; Marazzi, Monica Gioia; Thiébat, Gabriele; Romanelli, Massimiliano Marco Corsi; Sansone, Valerio

doi:10.1007/s00167-014-3143-x

Cited by 36 publications

(39 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mRNA levels of inflammatory cytokines are remarkably upregulated in injured tendons, the inhibition of IL‐1β via PEMF actuation could be beneficial for tendon‐healing strategies. These outcomes are consistent with those of other studies applying magnetic forces combined with 5, 17, or 75 Hz frequencies . Accordingly, the strong IL‐1β‐induction of MMP‐1 and MMP‐3 expression has been demonstrated in rabbit Achilles tendon cells exposed to cyclic strain and inflammatory cytokines .…”

Section: Discussionsupporting

confidence: 91%

“…In recent years, pulsed electromagnetic field (PEMF) actuation, which works by emitting a pulsating, varying intensity, and frequency electromagnetic field, was reported to modulate the cell processes. PEMF was shown to decrease pro‐inflammatory cytokines such as IL‐1β and tumor necrosis factor‐α (TNF‐α) and to increase anti‐inflammatory cytokines as IL‐10 by tendon cells in vitro, inhibited the synthesis of PGE2 and promoted anabolic activity of the chondrocytes . In addition, PEMF induced a downregulation of TNFα and factor nuclear factor‐κB (NF‐κB) on macrophages, evidencing the potential of PEMF‐actuated approaches for modulating inflammatory responses.…”

mentioning

confidence: 99%

“…In this study, hTDCs were stimulated with different concentrations of IL‐1β and exposed to six combinations of PEMF parameters, namely magnetic field intensity (1.5, 4, or 5 mT), frequency (5 or 17 Hz), and duty cycle (10% or 50%), some of which referred to hold potential in tendon inflammation‐related studies . As inflammation mediators have been associated to tendinopathic conditions, recreating the inflammatory cues of pathological environments combined with the potential of PEMF to modulate the inflammatory profile in hTDCs anticipates new opportunities approaching tendon regeneration.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Pulsed Electromagnetic Field Modulates Tendon Cells Response in IL‐1β‐Conditioned Environment

Vinhas

Rodrigues

Gonçalves

et al. 2019

Journal Orthopaedic Research

View full text Add to dashboard Cite

Strategies aiming at controlling and modulating inflammatory cues may offer therapeutic solutions for improving tendon regeneration. This study aims to investigate the modulatory effect of pulsed electromagnetic field (PEMF) on the inflammatory profile of human tendon‐derived cells (hTDCs) after supplementation with interleukin‐1β (IL‐1β). IL‐1β was used to artificially induce inflammatory cues associated with injured tendon environments. The PEMF effect was investigated varying the frequency (5 or 17 Hz), intensity (1.5, 4, or 5 mT), and duty‐cycle (10% or 50%) parameters to which IL‐1β‐treated hTDCs were exposed to. A PEMF actuation with 4 mT, 5 Hz and a 50% duty cycle decreased the production of IL‐6 and tumor necrosis factor‐α (TNF‐α), as well as the expression of TNFα, IL‐6, IL‐8, COX‐2, MMP‐1, MMP‐2, and MMP‐3, while IL‐4, IL‐10, and TIMP‐1 expression increased. These results suggest that PEMF stimulation can modulate hTDCs response in an inflammatory environment holding therapeutic potential for tendon regenerative strategies. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:160–172, 2020

show abstract

Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Pulsed Electromagnetic Field Modulates Tendon Cells Response in IL‐1β‐Conditioned Environment

Vinhas

Rodrigues

Gonçalves

et al. 2019

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Moreover, a slight increase in cell proliferation was observed in the same experimental setting [56, 57]. The anti-inflammatory effect of PEMFs was reported also in other cell types, such as rat BMSCs, where they were able to reduce the production of IL-1β and TNFα in a pathological model [27].…”

Section: Pulsed Electromagnetic Fieldmentioning

confidence: 87%

Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders

et al. 2016

View full text Add to dashboard Cite

BackgroundMusculoskeletal disorders are regarded as a major cause of worldwide morbidity and disability, and they result in huge costs for national health care systems. Traditional therapies frequently turned out to be poorly effective in treating bone, cartilage, and tendon disorders or joint degeneration. As a consequence, the development of novel biological therapies that can treat more effectively these conditions should be the highest priority in regenerative medicine.Main body of the abstractMesenchymal stem cells (MSCs) represent one of the most promising tools in musculoskeletal tissue regenerative medicine, thanks to their proliferation and differentiation potential and their immunomodulatory and trophic ability. Indeed, MSC-based approaches have been proposed for the treatment of almost all orthopedic conditions, starting from different cell sources, alone or in combination with scaffolds and growth factors, and in one-step or two-step procedures. While all these approaches would require cell harvesting and transplantation, the possibility to stimulate the endogenous MSCs to enhance their tissue homeostasis activity represents a less-invasive and cost-effective therapeutic strategy. Nowadays, the role of tissue-specific resident stem cells as possible therapeutic target in degenerative pathologies is underinvestigated. Biophysical stimulations, and in particular extracorporeal shock waves treatment and pulsed electromagnetic fields, are able to induce proliferation and support differentiation of MSCs from different origins and affect their paracrine production of growth factors and cytokines.Short conclusionsThe present review reports the attempts to exploit the resident stem cell potential in musculoskeletal pathologies, highlighting the role of MSCs as therapeutic target of currently applied biophysical treatments.

show abstract

“…17 Tendon cells isolated from human semitendinosus and gracilis tendons displayed altered regulation of gene expression dependent on field intensity, duration, and number of exposures. 4 In a rat model of Achilles tendon injury and repair, alterations in PEMF frequency and signal amplitude affected tendon strength after repair. 22 However, the effect of frequency and treatment duration has not yet been evaluated in our rat rotator cuff injury and repair animal model.…”

Section: Introductionmentioning

confidence: 99%

Effects of pulsed electromagnetic field therapy at different frequencies and durations on rotator cuff tendon-to-bone healing in a rat model

Huegel

Choi

Nuss

et al. 2018

Journal of Shoulder and Elbow Surgery

View full text Add to dashboard Cite

BACKGROUND Rotator cuff tears affect millions of individuals each year, often requiring surgical intervention. However, repair failure remains common. We have previously shown that pulsed electromagnetic field (PEMF) therapy improved tendon-to-bone healing in a rat rotator cuff model. The purpose of this study was to determine the influence of both PEMF frequency and exposure time on rotator cuff healing. METHODS 210 Sprague Dawley rats underwent acute bilateral supraspinatus injury and repair followed by either Physio-Stim® PEMF or High Frequency PEMF for 1, 3, or 6 hours daily. Control animals did not receive PEMF therapy. Mechanical and histological properties were assessed at 4, 8, and 16 weeks. RESULTS Improvements in different mechanical properties at various endpoints were identified for all treatment modalities when compared to non-treated animals, regardless of PEMF frequency or duration. Of note, one hour of Physio-Stim showed significant improvements in tendon mechanical properties across all time points, including increases in both modulus and stiffness as early as 4 weeks. Collagen organization improved for several of the treatment groups compared to controls. Additionally, improvements in collagen I and fibronectin expression were identified with PEMF treatment. Importantly, no adverse effects were identified in any mechanical or histological property. CONCLUSIONS Overall, results suggest that PEMF has a positive effect on rat rotator cuff healing for each electromagnetic fundamental pulse frequency and treatment duration tested in this study.

show abstract

In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field

Cited by 36 publications

References 42 publications

Pulsed Electromagnetic Field Modulates Tendon Cells Response in IL‐1β‐Conditioned Environment

Pulsed Electromagnetic Field Modulates Tendon Cells Response in IL‐1β‐Conditioned Environment

Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders

Effects of pulsed electromagnetic field therapy at different frequencies and durations on rotator cuff tendon-to-bone healing in a rat model

Contact Info

Product

Resources

About