In vitro flowering of Withania somnifera Dunal.—An important antitumor medicinal plant

Saritha, K.; Naidu, C. V.

doi:10.1016/j.plantsci.2006.12.016

Cited by 42 publications

(21 citation statements)

References 18 publications

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“…In many self-pollinating crops, seed setting followed by in vitro flowering has been previously reported (Asawaphan et al 2005;Bodhipadma and Leung 2003;Franklin et al 2000;Ignacimuthu et al 1997;Nadgauda et al 1990;Rao et al 2005;Saritha and Naidu 2007;Zhang 2007). Correspondingly, in the present study also mature lettuce seeds have developed in vitro ( Figure 1F).…”

Section: Transgenic Seed Setting In Vitrosupporting

confidence: 86%

“…In many species, in vitro flowers could produce seeds similar to the field-grown plants (Asawaphan et al 2005;Bodhipadma and Leung 2003;Franklin et al 2000;Ignacimuthu et al 1997;Ochatt and Sangwan 2008;Rao et al 2005;Saritha and Naidu 2007;Zhang 2007). Hence, in vitro flowering can also be used as a tool to reduce the seed generation time in functional genomics research, if viable seed setting is possible.…”

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confidence: 99%

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In vitro flowering and viable seed setting of transgenic lettuce cultures

Franklin

Oliveira

Dias

2011

Plant Biotechnology

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Production of transgenic lettuce seeds via in vitro flowering and fruit setting is reported here. Six days old cotyledons were co-cultivated with Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pCAMBIA2301 carrying the reporter gene a-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Transgenic calluses and shoot buds were induced on MS medium augmented with 0.1 mg l Ϫ1 benzyladenine (BA), 0.1 mg l Ϫ1 a-naphtaleneacetic acid (NAA), 100 mg l Ϫ1 kanamycin and 500 mg l Ϫ1 timentin (ticarcillin clavulanate). After transferring the cultures onto MS basal medium augmented or not with kanamycin, in vitro flower induction was observed within 90 days and eventually matured and produced seed pods. Pollen grains obtained from flowers induced on basal medium without kanamycin were heterogeneous containing both GUS positive and negative pollen grains. In the corresponding seed population, a Mendelian ratio (3 : 1) of gusA transgene segregation was observed. On the other hand, flowers that were induced on basal medium under kanamycin selection, all the pollen grains were GUS positives.

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Section: Transgenic Seed Setting In Vitrosupporting

confidence: 86%

mentioning

confidence: 99%

In vitro flowering and viable seed setting of transgenic lettuce cultures

Franklin

Oliveira

Dias

2011

Plant Biotechnology

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“…There are several reports available on in vitro flowering and fruiting in many plants such as Capsicum annuum (Bodhipadma and Leung, 2003), Cleome viscosa (Rathore et al, 2013), and Cucumis sativus (Sangeetha and Venkatachalam, 2014). In vitro flowering and fruiting has also been reported in W. somnifera (Saritha and Naidu, 2007), but the frequency of fruiting was low (7.5% with 1.0 fruit per plantlet), and attempts have not been made to studying the viability of seeds obtained from in vitro. Adventitious shoot regeneration, flowering and fruiting in vitro depends on the culture medium composition, culture conditions and plant growth regulators (PGRs) (Bodhipadma and Leung, 2003;Dewir et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Optimizing factors affecting adventitious shoot regeneration, in vitro flowering and fruiting of Withania somnifera (L.) Dunal

Sivanesan

Park

2015

Industrial Crops and Products

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“…The combination of BA and GA 3 induced direct in vitro flowering in ginseng (Lin et al 2005a). Regeneration of flowers and fruit was observed in Withania somnifera when incubated on MS medium with kinetin (KT) and indole-3-acetic acid (IAA) (Saritha and Naidu 2007). Several in vitro systems to stimulate flowering have been established in many plant species, together with types and levels of phytohormones to enhance the efficiency of flower induction.…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of inflorescence in Dioscorea zingiberensis

Huang

Yang

et al. 2009

Plant Cell Tiss Organ Cult

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Inflorescence induction and morphogenesis of regenerated flowers were investigated in vitro in Dioscorea zingiberensis C. H. Wright. Inflorescence induction was influenced by the type and concentration of phytohormones. When floral bud explants were incubated on a Murashige and Skoog medium containing a combination of 2.0 mg l -1 6-benzyladenine and 0.5 mg l -1 indole-3-butyric acid, the highest frequency of inflorescence induction was observed. However, in the presence of gibberellic acid, induction efficiency was reduced although node length of inflorescence was increased. Ontogenetic studies revealed that the inflorescence primordia originated directly from axillary epidermal cells of the perianth and bract of the explants after 7 days. In vitro, male flowers developed normally and blossomed after 90-100 days. In addition, some bisexual flowers were observed. These results demonstrated that there were differences in sexual differentiation of floral buds in vitro compared with that in vivo.

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In vitro flowering of Withania somnifera Dunal.—An important antitumor medicinal plant

Cited by 42 publications

References 18 publications

In vitro flowering and viable seed setting of transgenic lettuce cultures

In vitro flowering and viable seed setting of transgenic lettuce cultures

Optimizing factors affecting adventitious shoot regeneration, in vitro flowering and fruiting of Withania somnifera (L.) Dunal

In vitro induction of inflorescence in Dioscorea zingiberensis

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