In Vitro Flowering and Propagation of Bulbophyllum Auricomum Lindl., the Royal Flower of Myanmar

Than, Myo Ma Ma; Pal, A. B.; Jha, Sumita

doi:10.17660/actahortic.2009.829.14

Cited by 8 publications

(10 citation statements)

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“…Among in vitro raised seed derived plants, only in SC42 three polymorphic fragments of 0.65, 0.80 and 1.3 kb were amplified by primers OPA 07, 11 and 14 respectively. These plants produced precocious flowering under in vitro condition [39]. These three marker loci were also present in other culture regenerants that flowered in vitro, suggesting possibility of distinguishing in vitro flowering B. auricomum from the non-flowering group.…”

Section: Resultsmentioning

confidence: 72%

“…Seeds from immature capsules were germinated aseptically on Murashige and Skoog's (MS) basal medium [25] supplemented with coconut water. Shoots were transferred to MS medium supplemented with different combinations and concentrations of plant growth regulators (PGRs) for root induction and multiplication [39]. The complete plantlets developed under different PGR treatments were transferred to MS basal medium devoid of PGRs.…”

Section: Methodsmentioning

confidence: 99%

“…In nature, the germination frequency of seeds is very low (0.2-0.3%) and seedlings take long time to germinate. As a result of reckless harvest from the wild for commercial purposes there has been a steady decline in its natural population and like many other orchids listed in several national Red Data Books, B.auricomum is now in the verge of extinction [39].…”

Section: Introductionmentioning

confidence: 99%

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Genomic variations among in vitro regenerated Bulbophyllum auricomum Lindl. plants

Than

Majumder

Pal

et al. 2011

Nucleus

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Bulbophyllum auricomum Lindl. is a commercially important, endangered orchid in Myanmar. Maintenance of genetic variation is considered essential for the long-term survival of a species. In the present study, DNA fingerprinting profile was used to evaluate the genomic variations among 20 randomly selected in vitro regenerated seed derived plants. A total of twelve random primers have generated 101 polymorphic loci (69% of total amplified loci). Genetic polymorphisms between 20 randomly selected in vitro regenerated plants and the donor plant were used to generate a genetic distance matrix based on Nei's similarity coefficient. Genetic distances between the donor plant and culture regenerants were from 0.23 to 0.38, and 0.05 to 0.30 within 20 regenerants. Dendrogram constructed based on genetic distance matrix revealed two major sub-clusters accommodating all these 20 culture regenerants, while the donor plant appeared as an outgroup. Genomic variations developed among seed derived in vitro regenerated plants. The present findings indicate crosspollinating nature of the plant and presence of heterogeneous seed population. On the other hand, clonal propagation of in vitro raised plants maintained their clonal fidelity. It has been demonstrated that DNA fingerprinting profiles could be successfully employed to detect genomic variability, if any, at the early stages of shoot differentiation in this native endangered orchid.

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Section: Resultsmentioning

confidence: 72%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic variations among in vitro regenerated Bulbophyllum auricomum Lindl. plants

Than

Majumder

Pal

et al. 2011

Nucleus

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“…Optimum germination of around 90% was found when seeds were collected by 140 DAP [7]. In B. niphondii, fruit was harvested 12 months after pollination and stored at 10 0 C before use [10], in B. auricomum fruit was used 3-4 months after pollination [6] [12].…”

Section: Resultsmentioning

confidence: 99%

“…Bulbophyllum is one of the economic value orchids by its unique flowers and benefits. Several Bulbophyllum are interesting because of their uniqueness, such as B.auricomum [6], B. fascinator [7], B. macrantum, and B. phalaenopsis [8]. Several of them are useful as medicine because they contain secondary metabolites such as B. odoratissimum which is known to contain bulbophythrins that show cytotoxic activity against several human cells cancers such as leukemia, hepatoma, lung adenocarcinoma and stomach cell cancer [9] and several listed as rare orchids such as B. niphondii [10], B. dhaninivatii [11], B. auricomum [12].…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Bulbophyllum Orchids

Ulia,

Noli,

Idris

2023

IJPSAT

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Abstract— Bulbophyllum (Orchidaceae) is recognized as the largest genus of orchids in the world. Bulbophyllum is a popular orchid by its unique flowers and its benefits. Although Bulbophyllum is a large genus, research on in vitro propagation of orchids from this genus is still limited. This article aims to examine the micropropagation of several Bulbophyllum species to determine the affecting factor for the propagation of Bulbophyllum orchids. A systematic Literature Review (SLR) uses in this paper. The micropropagation of Bulbophyllum have studied in B.auricomum, B. fascinator, B. macrantum, B. phalaenopsis, B. lasianthum B. odoratissimum B. niphondii, B. dhaninivatii, B. lilacinum, B. echinolabium, B. putidum and B. plumatum. The use of appropriate media can increase the growth of orchids. The addition of growth regulators and/or organic compounds to a suitable basal medium can be a way to find a suitable medium for orchid growth. Acclimatization should be conducted on plantlets with healthy roots. The appropriate acclimatization media can increase the survival rate of orchid seedlings.

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Micropropagation of Orchids

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In Vitro Flowering and Propagation of Bulbophyllum Auricomum Lindl., the Royal Flower of Myanmar

Cited by 8 publications

References 0 publications

Genomic variations among in vitro regenerated Bulbophyllum auricomum Lindl. plants

Genomic variations among in vitro regenerated Bulbophyllum auricomum Lindl. plants

Micropropagation of Bulbophyllum Orchids

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