In vitro Fibroblast and Dermis Fibroblast Activation by Laser Irradiation at Low Energy

Bosatra, M; Jucci, A; Olliaro, P; Quacci, Daniela Elena; Sacchi, Silvia

doi:10.1159/000249691

Cited by 82 publications

(35 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this work, Hardy et al [29] reported a five-fold increase in the number of fibroblasts exposed to four 10.0 J cm -2 doses of pulsed ruby laser when compared to controls that were not treated with laser light. Subsequent studies have since shown that the increased proliferation of fibroblasts results in increased collagen synthesis in vitro as well as in vivo [30][31][32][33][34][35][36][37][38]. For example, a total of 4.0 joules laser energy applied on experimentallyinduced skin incisions [39] or skin defects in rats [30], produces faster collagen synthesis as judged by assaying 14 C glycine and 3H proline or 3H hydroxyproline [30,39].…”

Section: Effects Of Laser Photostimulationmentioning

confidence: 99%

The Biological Effects of Laser Therapy and Other Physical Modalities on Connective Tissue Repair Processes

Enwemeka

Reddy

2000

LASER THERAPY

View full text Add to dashboard Cite

Connective tissue injuries, such as tendon rupture and ligamentous strains, are common. Unlike most soft tissues that require 7-10 days to heal, primary healing of tendons and other dense connective tissues take as much as 6 -8 weeks during which they are inevitably protected in immobilization casts to avoid re-injury. Such long periods of immobilization impair functional rehabilitation and predispose a multitude of complications that could be minimized if healing is quickened and the duration of cast immobilization reduced. In separate studies, we tested the hypothesis that early function, ultrasound, 632.8 nm He-Ne laser, and 904 nm Ga-As laser, when used singly or in combination, promote healing of experimentally severed and repaired rabbit Achilles tendons as evidenced by biochemical, biomechanical, and morphological indices of healing. Our results demonstrate that: (1) appropriate doses of each modality, i.e., early functional activities, ultrasound, He-Ne and Ga-As laser therapy augment collagen synthesis, modulate maturation of newly synthesized collagen, and overall, enhance the biomechanical characteristics of the repaired tendons. (2) Combinations of either of the two lasers with early function and either ultrasound or electrical stimulation further promote collagen synthesis when compared to functional activities alone. However, the biomechanical effects measured in tendons receiving the multi-therapy were similar, i.e., not better than the earlier single modality trials. Although tissue repair processes in humans may differ from that of rabbits, these findings suggest that human cases of connective tissue injuries, e.g., Achilles tendon rupture, may benefit from appropriate doses of He-Ne laser, Ga-As laser, and other therapeutic modalities, when used singly or in combination. Our recent meta-analysis of the laser therapy literature further corroborate these findings.

show abstract

Section: Effects Of Laser Photostimulationmentioning

confidence: 99%

The Biological Effects of Laser Therapy and Other Physical Modalities on Connective Tissue Repair Processes

Enwemeka

Reddy

2000

LASER THERAPY

View full text Add to dashboard Cite

show abstract

“…Investigations have proved that the He-Ne laser irradiation of 25-mW output had a direct influence on human fibroblasts of 32nd passage (Bosatra et al, 1984). The synthesis of fibrillar material was stimulated, but collagenogenesis was not influenced.…”

Section: Action On Proliferative Activitymentioning

confidence: 99%

Effects of Visible Radiation on Cultured Cells

Karu

1990

Photochem & Photobiology

170

118

View full text Add to dashboard Cite

“…PGE 2 injection causes local pain and severe root resorption [4], and frequent injections of 1,25-(OH) 2 D 3 or PTH may induce unfavorable general conditions. Recently, various biostimulatory effects of lowenergy laser irradiation that involve wound healing [5][6][7], fibroblast [8][9][10][11] and chondral proliferation [12], collagen synthesis [13][14][15][16], and nerve regeneration [17] have been reported. In particular, the acceleration of bone regeneration by laser treatment has been a focus of contemporary research [18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats

2000

View full text Add to dashboard Cite

Background and Objective: Low-energy laser irradiation has many anabolic effects such as the acceleration of bone formation. However, its effects on tooth movement, performed by bone resorption and formation, have not been well characterized. Study Design/Materials and Methods: A total of 10 g of orthodontic force was applied to rat molars to cause experimental tooth movement. A Ga-Al-As diode laser was used to irradiate the area around the moved tooth, and after 12 days, the amount of tooth movement was measured. Calcein was injected subcutaneously to label the newly formed alveolar bone for quantitative analysis. Immunohistochemical staining of proliferating cell nuclear antigen was performed to evaluate cellular proliferation. TRAPase staining was also performed to facilitate the identification of osteoclasts. Results: In the laser irradiation group, the amount of tooth movement was significantly greater (1.3-fold) than that of the nonirradiation group in the end of the experiment. The amount of bone formation and rate of cellular proliferation in the tension side and the number of osteoclasts in the pressure side were all significantly increased in the irradiation group when compared with the nonirradiation group (P < 0.01). Conclusion:These findings suggest that low-energy laser irradiation can accelerate tooth movement accompanied with alveolar bone remodeling.

show abstract

In vitro Fibroblast and Dermis Fibroblast Activation by Laser Irradiation at Low Energy

Cited by 82 publications

References 0 publications

The Biological Effects of Laser Therapy and Other Physical Modalities on Connective Tissue Repair Processes

The Biological Effects of Laser Therapy and Other Physical Modalities on Connective Tissue Repair Processes

Effects of Visible Radiation on Cultured Cells

Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats

Contact Info

Product

Resources

About