In vitro fertilization of in vitro matured pig oocytes

Wu, Guoxuan; Qin, P.C.; Tan, Jing; Wang, L.A.

doi:10.1016/0093-691x(92)90392-5

Cited by 6 publications

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“…Different data h a v e b e e n p u b l i s h e d a b o u t t h e a g e a n d reproductive stage of oocyte donors. While some authors did not find a significant difference between oocyte maturation ability of prepuberal and puberal gilts [5,7], other reports did not mention the age of slaughtered donors of oocytes [6]. Nevertheless, there is some evidence that the fertility of cumulus oocyte complexes (COCs) collected from puberal gilts is better than that of prepuberal animals [11][12][13].…”

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“…The first IVF piglets were born after in vivo maturation of oocytes, however IVM prior to IVF was found to be less effective [3,4]. Successful IVF programs following IVM were reported later, but the outcomes of such trials were much less reliable than that of IVF after in vivo maturation [5][6][7]. Approximately 90% of oocytes selected according to the cytoplasm and cumulus cells completed nuclear maturation [8], but artificial milieu affected this process [9,10].…”

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Ovarian Activity and Oocyte Development during Follicular Development in Pigs at Different Reproductive Phases Estimated by the Repeated Endoscopic Method

Rátky¹,

Torner

Egerszegi³

et al. 2005

Journal of Reproduction and Development

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Abstract. The aim of the present study was to assess follicular and oocyte development in the same gilts during three phases of their reproductive life [prepuberal gilts (PP; 6.0 months of age), puberal gilts (P; 9.5 months of age) and primiparous sows (S)]. Follicular development was stimulated by the injection of 1,000 IU of equine chorionic gonadotropin (eCG) followed by 500 IU of human chorionic gonadotropin (hCG) 72 h later. Cumulus-oocyte-complexes (COCs) were recovered by endoscopic ovum pick up/aspiration from preovulatory follicles of the left ovary, and the follicular fluid (FF) from the right ovary was collected 34 h after the hCG treatment by endoscopy. Altogether, 19 pigs were used in the PP and P trials and 12 in the S trial. From the left ovaries, 168, 190 and 82 follicles were aspirated and 106, 125 and 42 COCs, respectively, were recovered (recovery rate 61 ± 27, 63 ± 21 and 53 ± 22%, respectively). The mean number of follicles was greater in the P phase than in the PP phase (19.7 ± 6.8 vs. 15.7 ± 6.8; p=0.06) and S phases (14.2 ± 4.0; p<0.05). More uniform oocytes with an expanded cumulus were aspirated in the P and PP phases than in the S phase (90 and 78 vs. 46%; p<0.05). Furthermore, the meiotic configuration in oocytes (T I/M II stage) differed between the three phases (56 and 62 vs. 0%; p<0.05). Progesterone (P4) levels in FF decreased from 590.0 ± 333.6 (PP) to 249.1 ± 72.6 (P) and 161.4 ± 75.2 ng/ml (S) (p<0.05). Estradiol-17β (E2) levels differed between PP and P gilts and S sows (9.3 ± 2.9, 21.9 ± 10.6 and 94.0 ± 15.9 pg/ml, respectively; p< 0.05), and the P4/E2 ratio was 72, 15 and 5, respectively. These results indicate differences in follicular and oocyte development between the reproductive phases investigated. Puberal gilts should preferably be used in IVF and breeding programs. The lower reproductive potential of primiparous sows must be taken into consideration in breeding. Any prediction of lifetime performance based on individual ovarian reactions of prepuberal gilts is unreliable. Key words: Endoscopy, Follicular fluid, Oocyte morphology, Sow, Steroid hormone (J. Reprod. Dev. 51: [109][110][111][112][113][114][115] 2005) early 50 years ago, it was recognized that oocytes were restricted in prophase I of meiosis till the ovulatory period [1]. Since the resumption of meiosis is triggered by the preovulatory luteinizing hormone (LH) peak which can be stimulated with exogenous gonadotropins, the most essential steps of oocyte maturation occur during the last couple of days before ovulation [2]. In the 1980-s, there were improvements to in vitro

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confidence: 99%

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confidence: 99%

Ovarian Activity and Oocyte Development during Follicular Development in Pigs at Different Reproductive Phases Estimated by the Repeated Endoscopic Method

Rátky¹,

Torner

Egerszegi³

et al. 2005

Journal of Reproduction and Development

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“…Research and initial application in the field of gene manipulation to improve growth and disease resistance, to generate foreign proteins in blood and milk, and to create tissues and organs for xenotransplantation need embryo transfer [7,18,19,35,71,95]. Other reproductive techniques to produce offspring like ovum pick up, and in vitro maturation and fertilization [14,64,68,87,110,113], generation of sexed embryos [88,89], cryoconservation of embryos [26, 41,46] and cloning [12,69,80,94] require also successful handling of embryo transfer technique. However, these reproductive techniques did not find practical application in swine production, yet.…”

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confidence: 99%

In vitro technologies related to pig embryo transfer

et al. 2000

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Changes in germinal vesicle (GV) chromatin configurations during growth and maturation of porcine oocytes

Sun

Liu

Yue

et al. 2004

Molecular Reproduction Devel

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Changes in germinal vesicle (GV) chromatin configurations during growth and maturation of porcine oocytes were studied using a new method that allows a clearer visualization of both nucleolus and chromatin after Hoechst staining. The GV chromatin of porcine oocytes was classified into five configurations, based on the degree of chromatin condensation, and on nucleolus and nuclear membrane disappearance. While the GV1 to 4 configurations were similar to those reported by previous studies, the GV0 configuration was distinct by the diffuse, filamentous pattern of chromatin in the whole nuclear area. Most of the oocytes were at the GV0 stage in the <1 and 1-1.9 mm follicles, but the GV0 pattern disappeared completely in the 2-2.9 and 3-6 mm follicles. As follicles grew, the number of oocytes with GV1 configurations increased and reached a maximum in the preovulatory follicles 4 hr post-hCG injection. During maturation in vivo, the number of GV1 oocytes decreased while oocytes undergoing GVBD increased. The percentage of oocytes with GV3 and GV4 configurations was constant during oocyte growth except at the 2-2.9 mm follicle stage, but these configurations disappeared completely after hCG injection. On the contrary, the in vitro maturing oocytes showed a large proportion of GV3 and GV4 configurations. There was no significant difference in distribution of chromatin configurations between the nonatretic and atretic follicles, and between oocytes with more than two layers of cumulus cells and those with less than one layer or no cumulus cells. Overall, our results suggested that (i) the GV0 configuration in porcine oocytes corresponded to the "nonsurrounded nucleolus" pattern in mice and other species; (ii) all the oocytes were synchronized at the GV1 stage before GVBD and this pattern might, therefore, represent a nonatretic state; (iii) the GV3 and GV4 configurations might represent stages toward atresia, or transient events prior to GVBD that could be switched toward either ovulation or atresia, depending upon circumstances; (iv) the in vitro systems currently used were not favorable for oocytes to switch toward ovulation (or final maturation); (v) the number of cumulus cells was not correlated with the chromatin configuration of oocytes, indicating that the beneficial effect of cumulus cells on oocyte maturation and development may simply be attributed to their presence during in vitro culture.

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In vitro fertilization of in vitro matured pig oocytes

Cited by 6 publications

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Ovarian Activity and Oocyte Development during Follicular Development in Pigs at Different Reproductive Phases Estimated by the Repeated Endoscopic Method

Ovarian Activity and Oocyte Development during Follicular Development in Pigs at Different Reproductive Phases Estimated by the Repeated Endoscopic Method

In vitro technologies related to pig embryo transfer

Changes in germinal vesicle (GV) chromatin configurations during growth and maturation of porcine oocytes

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