In vitro fertilization-intracytoplasmic sperm injection split: an insemination method to prevent fertilization failure

Hershlag, Avner; Paine, T. D.; Kvapil, Georgina; Feng, Haoran; Napolitano, Barbara

doi:10.1016/s0015-0282(01)02978-8

Cited by 84 publications

(52 citation statements)

References 10 publications

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“…This approach is often recommended despite the data that shows that fertilization rate and treatment outcome should be comparable with IVF and ICSI when the semen parameters are in the normal range and ICSI improves fertilization rates only when the semen parameters are borderline or when the morphology is poor [19][20][21][22]. Several studies have reported lower fertilization rates with IVF versus ICSI and a higher risk for complete fertilization failure with IVF among couples with unexplained infertility undergoing IVF following failed IUIs [15][16][17][18]23]. Ruiz et al, on the other hand found no significant difference between IVF and ICSI fertilization rates among women with unexplained infertility in their prospective randomized trial [24].…”

Section: Discussionmentioning

confidence: 99%

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The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility

Kovács

Kováts

Sajgo

et al. 2010

J Assist Reprod Genet

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Purpose Patients with unexplained infertility may have fertilization problems. Split fertilization (ICSI and conventional IVF on sibling oocytes) is often used to avoid poor fertilization. Our aim was to assess the ability of hyaluronic acid binding (HA-binding) assay to predict spontaneous fertilization during IVF. Methods Prospective, blinded, controlled trial. Patients undergoing their first IVF cycle for unexplained infertility were eligible. Split fertilization was used. IVF and ICSI fertilization rates and embryo development based on 3 HAbinding cut-offs (< 60%; 60-80%; >80%) were compared. Results ICSI fertilization was higher than IVF, but none of the HA-binding cut-off levels predicted those cases where IVF was less effective, therefore ICSI only would have lead to improved outcome. Embryo development and morphology were similar in all cut-off groups. Conclusions HA-binding did not predict spontaneous fertilization in patients with unexplained infertility undergoing IVF treatment. When it was used for "screening" it did not help to select the method of fertilization.

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Section: Discussionmentioning

confidence: 99%

“…We have been using ICSI fertilization on half of the eggs and conventional IVF fertilization on the other half in cases of unexplained infertility. Other groups have recommended this approach as well [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility

Kovács

Kováts

Sajgo

et al. 2010

J Assist Reprod Genet

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show abstract

“…ICSI is powerful for treating male-factor infertility that could not be assisted using conventional IVF. However, unexpected fertilization failures from IVF in couples with normal semen may occasionally occur (14,15). There are no absolute parameters or sperm function tests to predict fertilization outcome for apparently normal semen.…”

Section: Discussionmentioning

confidence: 99%

“…Some investigators attempted IVF-ICSI split on fresh sibling oocytes as the insemination method for normal semen to prevent fertilization failures (14,15). For couples with tubal or unexplained infertility, they found that the use of ICSI rescued 9-16% of cycles where IVF failed.…”

Section: Discussionmentioning

confidence: 99%

Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization

Chen

Lien

Chang

et al. 2004

J Assist Reprod Genet

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Purpose : To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle. Methods : The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed. Results : After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX. Conclusions : In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.

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“…Plachot et al (2002) compared the fertilisation outcome of 58 first IVF couples with mild male infertility, among which 19 (32.8%) resulted in fertilisation after ICSI only and 39 (67.2%) with fertilisation in both IVF and ICSI. In another study performed by Hershlag et al (2002), complete fertilisation failure after conventional IVF occurred in 10 of 60 couples (16.7%) with unexplained infertility and in 2 of 50 couples (4%) with borderline semen characteristics. Results from the above reports suggest that fertilisation could be successfully achieved in the large majority of patients with mild male infertility or borderline semen.…”

Section: Introductionmentioning

confidence: 93%

The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen

Jin¹,

Shu²,

Dai³

et al. 2014

Reprod. Fertil. Dev.

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Abstract. In this study we evaluated the value of short-time insemination and early rescue intra-cytoplasmic sperm injection (ICSI) in preventing the occurrence of complete fertilisation failure for mild or moderate male infertility patients. A total of 866 couples with borderline semen who underwent in vitro fertilisation treatment in 2010 were included. Regular insemination was performed between January and June of 2010 and short-term insemination was performed from July through December 2010, where, as early as 4 h after insemination, oocytes were denuded from cumulus cells and extrusion of the second polar body was evaluated. Of the 4153 mature oocytes with a detectable second polar body 4 h after insemination, 3874 (93.3%) showed signs of fertilisation on Day 1. Where no second polar body was present in any of the retrieved oocytes for a given patient, rescue ICSI was performed immediately. Similar rates of normal fertilisation and percentage of good-quality embryos were obtained between early rescue ICSI and regular ICSI. Clinical pregnancy occurred in 16 of 43 patients (37.2%) receiving early rescue ICSI. Our results showed early rescue ICSI in combination with evaluation of the second polar body 4 h following insemination is an effective method to prevent complete fertilisation failure for patients with mild or moderate male infertility.Additional keywords: borderline semen, clinical outcome, early rescue ICSI, failed fertilization, second polar body.

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In vitro fertilization-intracytoplasmic sperm injection split: an insemination method to prevent fertilization failure

Cited by 84 publications

References 10 publications

The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility

The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility

Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization

The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen

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