In vitro fertilization and cleavage capability of bovine follicular oocytes classified by cumulus cells and matured in vitro

Shioya, Yasuo; Kuwayama, Masashige; Fukushima, Moriyuki; Iwasaki, Setsuo; Hanada, Akira

doi:10.1016/0093-691x(88)90198-7

Cited by 87 publications

(42 citation statements)

References 9 publications

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“…Oocytes with several layers of cumulus cells had a significantly higher percentage of cleavage and transferable embryos. Similar results were previously recorded in cattle [7,24,25]. Cumulus cells improve the fertilization in cattle by providing a capacitation-inducing mechanism, and facilitating the interaction between capacitated spermatozoa and the zona pellucida surface [6,7,26].…”

Section: Discussionsupporting

confidence: 77%

New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

Kandil

Abdoon

Murakami

et al. 1999

Journal of Reproduction and Development

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Abstract. Buffalo ovaries were collected from a slaughter house and cumulus oocyte complexes were aspirated from follicles 2-6 mm in diameter. A portable CO2 incubator and a CR1aa medium were used for in vitro production of embryos. In the first experiment, numbers of follicles and oocytes per ovary were counted, and classified according to the appearance of the cumulus cells. In the second experiment, in vitro fertilization and cultivation of buffalo oocytes matured in vitro were carried out using semen from three different bulls, which had different sperm motilities (Bull 1: 20%, Bull 2: 60% and Bull 3: 15%, respectively). In the third experiment, the cleavage and transferable embryos of fertilized oocytes from denuded oocytes, and oocytes with intact cumulus cell layers were compared. The percentages of good, fair and poor quality oocytes were 60.9%, 17.8% and 21.4%, respectively. The rates of cleavage and transferable embryos were significantly higher after incubation with semen from Bull 2 (81% and 40.2%) than those after incubation with semen from Bulls 1 (52% and 13.5%, p<0.05 and p<0.01) and 3 (15.8% and 0%, p<0.01). The rates of cleavage and transferable embryos were significantly higher (p<0.01) for oocytes with an intact cumulus than for oocytes without cumulus cells at fertilization.

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Section: Discussionsupporting

confidence: 77%

New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

Kandil

Abdoon

Murakami

et al. 1999

Journal of Reproduction and Development

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“…A implicação da PK-C na maturação do oócito tem sido demonstrada em diversas espécies animais. Tem sido sugerido que ela inibe a divisão meiótica em oócitos de camundongo (Bornslaeger et al, 1986;Gonçalves e Graves, 1992;Lu et al, 2001) e de Xenopus (Stith et al, 1992), porém, deve ser ativada para que o oócito de camundongo passe a MII (Colonna et al, 1997). A PK-C estimula a maturação nuclear de oócitos em ratos (Aberdam e Dekel, 1985), rã (Rana dybowskii) (Kwon e Lee, 1991), peixes (killifish) (Cerdá et al, 1998), bovinos (Vignola, 1995;Montagner et al, 1999;Rose-Hellekant e Bavister, 1996) e suínos (Su et al, 1999), mas não está envolvida no reinício da meiose de oócitos de corvina (Patiño et al, 2003).…”

Section: Introductionunclassified

“…As células do complexo cumulus-oócito (CCO) exercem importante papel na regulação da maturação do oócito e a retirada dessas células reduz significativamente o desenvolvimento embrionário subseqüente (Zhang et al, 1995;Shioya et al, 1998). Há evidência suficiente da importância da PK-C no processo de maturação de oócitos.…”

Section: Introductionunclassified

Interação entre células do cumulus e atividade da proteína quinase C em diferentes fases da maturação nuclear de oócitos bovinos

Bertagnolli

Gonçalves

Giometti

et al. 2004

Arq. Bras. Med. Vet. Zootec.

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RESUMOVerificou-se a influência da proteína quinase C (PK-C) no reinício e na progressão da meiose em oócitos bovinos, determinando se as células do cumulus são mediadoras da PK-C na regulação da maturação dos oócitos. Complexos cumulus-oócitos (CCO) e oócitos desnudos (OD), distribuídos aleatoriamente em seis tratamentos (T) com base na presença de um ativador da PK-C (PMA) (T1 e T2), de um forbol éster incapaz de ativar a PK-C (4α-PDD-controle) (T3 e T4) ou de apenas o meio básico (TCM-199-controle) (T5 e T6), foram cultivados por 7, 9, 12, 18 e 22 horas. A percentagem de rompimento da vesícula germinativa no grupo cultivado com PMA foi maior do que nos dois grupos controle, com e sem células do cumulus. O cultivo de CCO e OD por 12 e 18 horas demonstrou que a PK-C influencia a progressão para os estádios de metáfase I (MI) e metáfase II (MII) de maneira dependente das células do cumulus. Nos períodos de 9 e 22 horas, não foi possível observar diferença entre os grupos quanto aos diferentes estádios de maturação. A ativação da PK-C acelera o reinício da meiose independentemente das células somáticas e acelera a progressão até os estádios de MI e MII na dependência das células do cumulus.Palavras-chave: bovino, maturação nuclear de oócitos, PK-C medium (T5 and T6), were cultivated for 7, 9, 12, 18 and 22 ABSTRACT The aim of this study was to evaluate the effect of protein kinase C (PK-C) on the meiotic resumption and progression in bovine oocyte, and to determine if the cumulus cells mediate the PK-C action in the regulation of bovine oocyte nuclear maturation. Cumulus-oocyte complexes (COC) and denuded oocytes (DO), randomly allotted to 6 treatments (T) based on the presence of an activator of PK-C (PMA) (T1 and T2), or a phorbol ester unable to activate PK-C (4αPDD-control) (T3 and T4) or a basic culture

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“…After washing, COCs were classified into one of four morphological categories based primarily upon the characteristics of their cumulus cell investment, with slight modification of the criteria described by Shioya et al [5]. Class A oocytes had compact and dense cumulus cell layers (more than 3 layers thick).…”

Section: Oocyte Morphological Classificationmentioning

confidence: 99%

“…Oocyte quality is an important factor affecting the success of an IVP system. Selection of oocytes for in vitro maturation is generally based on the quality of the cytoplasm and the characteristics of the cumulus cell investment around the oocyte [4,5]. These morphological criteria are routinely used to select the cumulus-oocyte complexes (COCs) with the most competent oocytes for embryo development.…”

mentioning

confidence: 99%

A Simple Method for Selection of Cumulus-Oocyte Complexes from Bovine Ovaries by Sedimentation with Percoll

Yotsushima

Shimizu

Kon

et al. 2007

Journal of Reproduction and Development

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Abstract. Selection of bovine cumulus-oocyte complexes (COCs) for in vitro embryo production (IVP) is generally based on the morphological characteristics of the cumulus cells surrounding the oocytes and the ooplasm under microscopic observation. The purpose of this study was to examine a simple method for selection of COCs by sedimentation with Percoll solutions. COCs were aspirated from ovaries derived from a local slaughterhouse, and the COCs were classified by the morphology of their cumulus cell layers, as follows: Class A, compact and thick; Class B, compact but thin; Class C, partially denuded and thin; and Class D, denuded. Percoll solutions were prepared by diluting Percoll to 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5 and 30% solutions, respectively. COCs were placed on the surface of the Percoll solution for 3 min, and the precipitated COCs were transferred to stepwise high density solution. The percentage of Percoll solution just before buoyancy was considered to the specific sedimentary value of the COC and oocyte. The mean sedimentary value of Class A COCs was higher than those of the other classes (P<0.01). The mean sedimentary values of denuded oocytes from Classes A and B were higher than those from Classes C and D (P<0.01). Our results show that sedimentation of COCs and denuded oocytes was generally related to the morphological quality of the COCs, although the sedimentary values ranged widely for one class of COCs and oocytes. The Percoll method can be used for simple selection of COCs. Key words: Bovine, Cumulus-oocyte complex, Percoll, Sedimentation (J. Reprod. Dev. 53: [971][972][973][974][975][976] 2007) n vitro maturation, fertilization and culture technologies for bovine oocytes are beneficial for increasing the number of embryos transferable to recipient cows. Various factors, including oocyte quality, culture media, protein source, growth factors and oxygen tension for the in vitro embryo production system (IVP), affect preimplantation embryo development in vitro [1][2][3]. Oocyte quality is an important factor affecting the success of an IVP system. Selection of oocytes for in vitro maturation is generally based on the quality of the cytoplasm and the characteristics of the cumulus cell investment around the oocyte [4,5]. These morphological criteria are routinely used to select the cumulus-oocyte complexes (COCs) with the most competent oocytes for embryo development. However, subjective evaluation of oocyte quality by morphological criteria under microscopic observation is thought to differ among evaluators. Recently, other means of characterizing bovine oocytes have been examined, such as evaluating the corona radiata density of cumulus-corona-oocyte complexes [6], distribution of cortical granules in oocytes [7] and expression of the LH receptor in

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In vitro fertilization and cleavage capability of bovine follicular oocytes classified by cumulus cells and matured in vitro

Cited by 87 publications

References 9 publications

New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

Interação entre células do cumulus e atividade da proteína quinase C em diferentes fases da maturação nuclear de oócitos bovinos

A Simple Method for Selection of Cumulus-Oocyte Complexes from Bovine Ovaries by Sedimentation with Percoll

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