In vitro fermentation of human milk oligosaccharides by individual Bifidobacterium longum-dominant infant fecal inocula

Li, Hongye; Lane, Jonathan A.; Chen, Junchun; Lu, Zerong; Wang, Hongwei; Dhital, Sushil; Fu, Xiong; Huang, Qiang; Liu, Feitong; Zhang, Bin

doi:10.1016/j.carbpol.2022.119322

Cited by 23 publications

(18 citation statements)

References 63 publications

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“… 35 Meanwhile, it was closely correlated with the metabolism of a variety of prebiotics such as human milk oligosaccharides and galactooligosaccharides. 36 It was found that Bifidobactrium longum , Lactobacillus spp., appeared to be core species in the COL-C group, indicating that COL-C had stronger prebiotic effects than those of COL, which might be related to the enhanced contents of nutrition.…”

Section: Discussionmentioning

confidence: 99%

The in vitro fermentation of compound oral liquid by human colonic microbiota altered the abundance of probiotics and short-chain fatty acid production

et al. 2022

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Section: Discussionmentioning

confidence: 99%

The in vitro fermentation of compound oral liquid by human colonic microbiota altered the abundance of probiotics and short-chain fatty acid production

et al. 2022

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“…42 However, a recent study has reported no significant differences in gas production among different types of HMOs fermented by infant fecal inoculum. 43 These results indicated that the fermentation efficiency of HMOs was associated with other factors, such as microbial composition. It is well known that, in the small intestine of growing pigs, especially in the jejunum, Lactobacillus is the most dominant genus, and the number of Bifidobacteria is low.…”

Section: Discussionmentioning

confidence: 86%

Effects of 2'‐fucosyllactose on the composition and metabolic activity of intestinal microbiota from piglets after in vitro fermentation

Zhang,

Ye,

Guo

et al. 2023

J Sci Food Agric

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BackgroundAs indigestible carbohydrates, milk oligosaccharides exert various benefits for newborns mainly through intestinal microbiota, among which 2’‐fucosyllactose (2’‐FL) is the most predominant milk oligosaccharide. However, knowledge about the fermentative characteristics of 2’‐FL in the gut remains limited, especially in the small intestine. Therefore, this study aims to explore the differential fermentability of 2’‐FL by the small and large intestinal microbiota of piglets using fructo‐oligosaccharide (FOS) and lactose considered as the positive control groups by an in vitro batch fermentation experiment. During the fermentation, the microbial composition was characterized along with gas production and short‐chain fatty acid production.Results2’‐FL showed differential fermentability in the jejunal and colonic fermentation. Compared with the colon, 2’‐FL produced less gas in the jejunum, which was less than in the FOS and lactose groups (P < 0.05). Meanwhile, 2’‐FL exhibited a different influence on the microbial composition and metabolism in the jejunum and colon compared with FOS and lactose. In the jejunum, compared with the FOS and lactose groups, the 2’‐FL group showed a higher abundance of Bacteroides, Prevotella, and Blautia but a lower abundance of Streptococcus and Lactobacillus (P < 0.05), with a higher level of propionate and lower level of lactate during fermentation (P < 0.05). In the colon, compared with the FOS and lactose groups, 2’‐FL increased the abundance of Blautia, Faecalibacterium, and Lachnospiraceae FCS020 groups but decreased the abundance of Prevotella_9, Succinivibrio, and Megasphaera (P < 0.05) with the increase in acetate production (P < 0.05).ConclusionOverall, the results suggested that the small intestinal microbiota had a potential ability to ferment the milk oligosaccharides. Meanwhile, compared with FOS and lactose, 2’‐FL selectively stimulated the growth of propionate‐producing bacteria in the jejunum and acetate‐producing bacteria in the colon. These results demonstrated the differences in fermentation properties of 2’‐FL by the small and large intestinal microbiota and provided new evidence for the application of 2’‐FL in optimizing gut microbiota.This article is protected by copyright. All rights reserved.

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“…Breast milk has considerable implications in the gut microbiota of infants. , Numerous research has found that HMOs in human milk are the main driver in shaping the human gut microbiome . HMOs could resist gastric acid digestion and enter the distal small bowel at high concentrations, thus affecting the make of the intestinal flora or improving metabolism. , In particular, HMOs support the development of several Bifidobacterium species and antagonize the effect of pathogenic bacteria. , Therefore, HMOs maintain the ecological balance of the intestinal tract and serve as a prebiotic. For example, in vitro fermentation of major infant Bifidobacterium species with HMOs was first reported by Asakuma et al They reported that B. bifiudm and B. longum subsp.…”

Section: The Relationship Between Hmos and Gut Microbiotamentioning

confidence: 99%

“…63,64 In particular, HMOs support the development of several Bif idobacterium species and antagonize the effect of pathogenic bacteria. 65,66 Therefore, HMOs maintain the ecological balance of the intestinal tract and serve as a prebiotic. For example, in vitro fermentation of major infant Bif idobacterium species with HMOs was first reported by Asakuma et al They reported that B. bif iudm and B. longum subsp.…”

Section: Microbiotamentioning

confidence: 99%

Effects of Human Milk Oligosaccharides in Infant Health Based on Gut Microbiota Alteration

et al. 2023

J. Agric. Food Chem.

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The primary active components of breast milk are human milk oligosaccharides (HMOs). HMOs provide many benefits to infants, including regulating their metabolism, immune system, and brain development. Recent studies have emphasized that HMOs act as prebiotics by the metabolism of intestinal microorganisms to produce short-chain fatty acids, which are crucial for infant development. In addition, HMOs with different structural characteristics can form different microbial compositions. HMOsinduced predominant microbes, including Bif idobacterium infantis, B. bifidum, B. breve, and B. longum, and their metabolites demonstrated pertinent health-promoting properties. Meanwhile, HMOs could also directly reduce the occurrence of diseases through the effects of preventing pathogen infection. In this review, we address the probable function of HMOs inside the HMOsgut microbiota-infant network, by describing the physiological functions of HMOs and the implications of diet on the HMOs-gut microbiota-infant network.

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In vitro fermentation of human milk oligosaccharides by individual Bifidobacterium longum-dominant infant fecal inocula

Cited by 23 publications

References 63 publications

The in vitro fermentation of compound oral liquid by human colonic microbiota altered the abundance of probiotics and short-chain fatty acid production

The in vitro fermentation of compound oral liquid by human colonic microbiota altered the abundance of probiotics and short-chain fatty acid production

Effects of 2'‐fucosyllactose on the composition and metabolic activity of intestinal microbiota from piglets after in vitro fermentation

Effects of Human Milk Oligosaccharides in Infant Health Based on Gut Microbiota Alteration

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